1998
DOI: 10.1128/aac.42.9.2319
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Characterization of a PSE-4 Mutant with Different Properties in Relation to Penicillanic Acid Sulfones: Importance of Residues 216 to 218 in Class A β-Lactamases

Abstract: Class A β-lactamases are inactivated by the suicide inactivators sulbactam, clavulanic acid, and tazobactam. An examination of multiple alignments indicated that amino acids 216 to 218 differed among class A enzymes. By random replacement mutagenesis of codons 216 to 218 in PSE-4, a complete library consisting of 40,864 mutants was created. The library of mutants with mutations at positions 216 to 218 in PSE-4 was screened on carbenicillin and ampicillin with the inactivator sulbactam; a collection of 14 mutan… Show more

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Cited by 9 publications
(3 citation statements)
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“…Escherichia coli transformed with pMonTEM1 and pMonPSE4 were selected on LB‐agar plates containing a range of ampicillin concentrations (5 to 5000 μg/mL). Cells containing tem‐1 and pse‐4 grew on plates containing up to ∼1000 and 3000 μg/mL of ampicillin, respectively, similar to that previously reported (Sabbagh et al 1998). The minimum inhibitory concentration (MIC) of ampicillin for the strain of E. coli used was <5 μg/mL.…”
Section: Resultssupporting
confidence: 85%
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“…Escherichia coli transformed with pMonTEM1 and pMonPSE4 were selected on LB‐agar plates containing a range of ampicillin concentrations (5 to 5000 μg/mL). Cells containing tem‐1 and pse‐4 grew on plates containing up to ∼1000 and 3000 μg/mL of ampicillin, respectively, similar to that previously reported (Sabbagh et al 1998). The minimum inhibitory concentration (MIC) of ampicillin for the strain of E. coli used was <5 μg/mL.…”
Section: Resultssupporting
confidence: 85%
“…This process was repeated using the ligated products until the full‐length genes were assembled. After assembly, pse‐4 and tem‐1 were amplified by PCR and cloned into the vector pMon•1A2; this bacterial vector contains a constitutive PSE‐4 promoter and kanamycin selectable marker (Sabbagh et al 1998). Escherichia coli transformed with pMonTEM1 and pMonPSE4 were selected on LB‐agar plates containing a range of ampicillin concentrations (5 to 5000 μg/mL).…”
Section: Resultsmentioning
confidence: 99%
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