Characterization of a rice gene family encoding root-specific proteins

Xu, Yang; Buchholz, Wallace G.; DeRose, Richard T.; Hall, Timothy C.

doi:10.1007/bf00020180

Cited by 88 publications

(62 citation statements)

References 37 publications

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“…The GOS2 (rice eukaryotic translation initiation factor1-like gene) promoter has been shown to drive expression throughout the whole plant body (de Pater et al, 1992), while the RCc3 (rice lipid transfer proteinlike gene) promoter does so in a root-specific manner (Xu et al, 1995). Fifty independent lines for each construct were generated, and to eliminate somaclonal variation, successive field selection of T1 to T4 plants (4 years) was performed to identify elite lines that grew normally, without stunting.…”

Section: Oserf71 Overexpression In Rice Roots Enhances Drought Resistmentioning

confidence: 99%

Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance

et al. 2016

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Plant responses to drought stress require the regulation of transcriptional networks via drought-responsive transcription factors, which mediate a range of morphological and physiological changes. AP2/ERF transcription factors are known to act as key regulators of drought resistance transcriptional networks; however, little is known about the associated molecular mechanisms that give rise to specific morphological and physiological adaptations. In this study, we functionally characterized the rice (Oryza sativa) drought-responsive AP2/ERF transcription factor OsERF71, which is expressed predominantly in the root meristem, pericycle, and endodermis. Overexpression of OsERF71, either throughout the entire plant or specifically in roots, resulted in a drought resistance phenotype at the vegetative growth stage, indicating that overexpression in roots was sufficient to confer drought resistance. The root-specific overexpression was more effective in conferring drought resistance at the reproductive stage, such that grain yield was increased by 23% to 42% over wild-type plants or whole-body overexpressing transgenic lines under drought conditions. OsERF71 overexpression in roots elevated the expression levels of genes related to cell wall loosening and lignin biosynthetic genes, which correlated with changes in root structure, the formation of enlarged aerenchyma, and high lignification levels. Furthermore, OsERF71 was found to directly bind to the promoter of OsCINNAMOYL-COENZYME A REDUCTASE1, a key gene in lignin biosynthesis. These results indicate that the OsERF71-mediated drought resistance pathway recruits factors involved in cell wall modification to enable root morphological adaptations, thereby providing a mechanism for enhancing drought resistance.

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Section: Oserf71 Overexpression In Rice Roots Enhances Drought Resistmentioning

confidence: 99%

Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance

et al. 2016

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“…A widely used method in the past was the identification of highly homologous genes by genomic or cDNA library screenings (e.g. (Xu et al 1995;Sun et al 1999)). For this purpose hybridization can be applied or alternatively PCR-based methods, using degenerated oligonucleotides.…”

Section: Bottom-up Approachmentioning

confidence: 99%

Mechanisms of vegetative propagation in bulbs : a molecular approach

Moreno-Pachon

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“…A widely used method in the past was the identification of highly homologous genes by genomic or cDNA library screenings (e.g. (Sun et al, 1999, Xu et al, 1995). For this purpose hybridization can be applied or alternatively PCRbased methods, using degenerated oligonucleotides.…”

Section: Bottom-up Approachmentioning

confidence: 99%

Transfer of knowledge about flowering and vegetative propagation from model species to bulbous plants

Leeggangers¹,

Moreno-Pachon²,

Gude³

et al. 2013

Int. J. Dev. Biol.

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The extensive characterization of plant genes and genome sequences summed to the continuous development of biotechnology tools, has played a major role in understanding biological processes in plant model species. The challenge for the near future is to generate methods and pipelines for an efficient transfer of this knowledge to economically important crops and other plant species. In the case of flower bulbs, which are economically very important for the ornamental industry, flowering time control and vegetative propagation constitute the most relevant processes for agronomical improvements. Those processes have been reasonably studied in reference species, making them excellent candidates for translational investigations in bulbous plant species. The approaches that can be taken for the transfer of biological knowledge from model to non-model species can be roughly categorized as "bottom-up" or "top-down". The former approach usually goes from individual genes to systems, also known as a "gene-by-gene" approach. It assumes conservation of molecular pathways and therefore makes use of sequence homology searches to identify candidate genes. "Top-down" methodologies go from systems to genes, and are e.g. based on large scale transcriptome profiling via heterologous microarrays or RNA sequencing, followed by the identification of associations between phenotypes, genes, and gene expression patterns and levels. In this review, examples of the various knowledge-transfer approaches are provided and pros and cons are discussed. Due to the latest developments in transgenic research and next generation sequencing and the emerging of systems biology as a matured research field, transfer of knowledge concerning flowering time and vegetative propagation capacity in bulbous species are now within sight.

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Characterization of a rice gene family encoding root-specific proteins

Cited by 88 publications

References 37 publications

Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance

Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance

Mechanisms of vegetative propagation in bulbs : a molecular approach

Transfer of knowledge about flowering and vegetative propagation from model species to bulbous plants

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