Characterization of a second functional gene cluster for the catabolism of phenylacetic acid in Pseudomonas sp. strain Y2

Bartolomé-Martı́n, David; Martínez‐García, Esteban; Mascaraque, Victoria; Rubio, Jerónimo; Perera, Julián; Alonso, Sergio

doi:10.1016/j.gene.2004.06.042

Cited by 26 publications

(21 citation statements)

References 34 publications

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“…Y2 (Bartolomé-Martin et al, 2004;Velasco et al, 1998). The genes encoding the lower pathway (PACoA catabolon) are typically organized in five contiguous operons: paaABCEF, paaGHIJK, paaLMN, paaX and paaY (Luengo et al, 2001).…”

Section: Styrene Degradation Proteinsmentioning

confidence: 99%

Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions

et al. 2009

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Pseudomonas putida CA-3 is a styrene-degrading bacterium capable of accumulating mediumchain-length polyhydroxyalkanoate (mclPHA) when exposed to limiting concentrations of a nitrogen source in the growth medium. Using shotgun proteomics we analysed global proteome expression in P. putida CA-3 supplied with styrene as the sole carbon and energy source under N-limiting (condition permissive for mclPHA synthesis) and non-limiting (condition non-permissive for mclPHA accumulation) growth conditions in order to provide insight into the molecular response of P. putida CA-3 to limitation of nitrogen when grown on styrene. A total of 1761 proteins were identified with high confidence and the detected proteins could be assigned to functional groups including styrene degradation, energy, nucleotide metabolism, protein synthesis, transport, stress response and motility. Proteins involved in the upper and lower styrene degradation pathway were expressed throughout the 48 h growth period under both nitrogen limitation and excess. Proteins involved in polyhydroxyalkanoate (PHA) biosynthesis, nitrogen assimilation and amino acid transport, and outer membrane proteins were upregulated under nitrogen limitation. PHA accumulation and biosynthesis were only expressed under nitrogen limitation. Nitrogen assimilation proteins were detected on average at twofold higher amounts under nitrogen limitation. Expression of the branched-chain amino acid ABC transporter was up to 16-fold higher under nitrogen-limiting conditions. Branched chain amino acid uptake by nitrogenlimited cultures was also higher than that by non-limited cultures. Outer membrane lipoproteins were expressed at twofold higher levels under nitrogen limitation. This was confirmed by Western blotting (immunochemical detection) of cells grown under nitrogen limitation. Our study provides the first global description of protein expression changes during growth of any organism on styrene and accumulating mclPHA (nitrogen-limited growth). INTRODUCTIONPseudomonas putida CA-3 has been reported to utilize styrene as a sole source of carbon and energy and to accumulate the biodegradable polymer medium-chainlength polyhydroxyalkanoate (mclPHA) when nitrogen (N) becomes limited in the growth medium (O'Connor et al., 1996;. These two abilities have been employed in the two-step chemo-biotechnological conversion of polystyrene, a non-biodegradable polymer, to mclPHA at laboratory scale (Ward et al., 2006). The process has been improved through the controlled feeding of N to the growth medium, which results in a twofold increase in biomass and a 1.4-fold increase in mclPHA accumulation (Goff et al., 2007).Molecular investigations of the styrene degradation pathway in this organism have been reported (Mooney et al., 2006b;O'Connor et al., 2001). Styrene metabolism in P. putida CA-3 proceeds via initial side chain oxidation and involves an upper pathway converting styrene to phenylacetic acid (PA) (O'Connor et al., 1995), and a lower pathway initiated via activation of PA to phenylace...

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Section: Styrene Degradation Proteinsmentioning

confidence: 99%

Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions

et al. 2009

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“…ADP1 (66.5 %) (Kowalchuk et al, 1994). Homologous paaE genes are also present in the paa clusters of P. putida strains Jiménez et al, 2002;Bartolomé-Martín et al, 2004). To determine whether PaaE was the enzyme catalysing the last step in the PA degradation pathway, i.e.…”

Section: Pa Consumption By Wild-type and Sucd Mutant Strainsmentioning

confidence: 99%

“…The PA pathway is the core of the phenylacetyl-CoA catabolon, a functional unit that integrates peripheral catabolic pathways that convert several structurally related aromatic compounds, such as styrene, 2-phenylethylamine, tropic acid, and phenylacetyl esters and amides, to the common intermediate phenylacetyl-CoA (Luengo et al, 2001). The PA pathway has also been described in several other Gram-negative bacteria, such as A. evansii Rost et al, 2002), other Pseudomonas strains (Bartolomé-Martín et al, 2004) and even Gram-positive bacteria (Navarro-Llorens et al, 2005) and the genus Thermus (Kunishima et al, 2005;Song et al, 2006). Therefore, this pathway appears to be widely distributed in bacteria and is the only pathway of aerobic PA degradation reported so far in these organisms.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the last step of the aerobic phenylacetic acid degradation pathway

et al. 2007

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“…strain Y2 (6,(11)(12)(13). In this study, we identified a putative PAA degradation gene cluster (paa) in P. putida F1 with a gene organization similar to those of the paa2 gene cluster in Pseudomonas sp.…”

mentioning

confidence: 97%

Taxis of Pseudomonas putida F1 toward Phenylacetic Acid Is Mediated by the Energy Taxis Receptor Aer2

Luu

Schneider

et al. 2013

Appl Environ Microbiol

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bThe phenylacetic acid (PAA) degradation pathway is a widely distributed funneling pathway for the catabolism of aromatic compounds, including the environmental pollutants styrene and ethylbenzene. However, bacterial chemotaxis to PAA has not been studied. The chemotactic strain Pseudomonas putida F1 has the ability to utilize PAA as a sole carbon and energy source. We identified a putative PAA degradation gene cluster (paa) in P. putida F1 and demonstrated that PAA serves as a chemoattractant. The chemotactic response was induced during growth with PAA and was dependent on PAA metabolism. A functional cheA gene was required for the response, indicating that PAA is sensed through the conserved chemotaxis signal transduction system. A P. putida F1 mutant lacking the energy taxis receptor Aer2 was deficient in PAA taxis, indicating that Aer2 is responsible for mediating the response to PAA. The requirement for metabolism and the role of Aer2 in the response indicate that P. putida F1 uses energy taxis to detect PAA. We also revealed that PAA is an attractant for Escherichia coli; however, a mutant lacking a functional Aer energy receptor had a wild-type response to PAA in swim plate assays, suggesting that PAA is detected through a different mechanism in E. coli. The role of Aer2 as an energy taxis receptor provides the potential to sense a broad range of aromatic growth substrates as chemoattractants. Since chemotaxis has been shown to enhance the biodegradation of toxic pollutants, the ability to sense PAA gradients may have implications for the bioremediation of aromatic hydrocarbons that are degraded via the PAA pathway.

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Characterization of a second functional gene cluster for the catabolism of phenylacetic acid in Pseudomonas sp. strain Y2

Cited by 26 publications

References 34 publications

Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions

Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions

Characterization of the last step of the aerobic phenylacetic acid degradation pathway

Taxis of Pseudomonas putida F1 toward Phenylacetic Acid Is Mediated by the Energy Taxis Receptor Aer2

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