Characterization of a strain of Serratia sp. with ixodicide activity against the cattle tick Rhipicephalus microplus

Castro-Saines, Edgar; Hernández-Ortíz, Rubén; Lagunes-Quintanilla, Rodolfo; Peña-Chora, Guadalupe

doi:10.1007/s10493-021-00640-4

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…MDK33 grouped with members of the Enterobacteriaceae family. Similar findings were reported by Castro-Saines et al [40], who characterized Serratia sp., reporting ixodicide activity against the cattle tick Rhipicephalus microplus, with 99.86% identity to recognized species. Although analyses of 16S rRNA sequences can be used to define species, this is not always the case because 16S rRNA genes may contain highly conserved regions that make discrimination between some species and subspecies within this group difficult [39], indicating the importance of including other housekeeping genes with powerful taxonomic indication.…”

Section: Discussionsupporting

confidence: 91%

First Isolation and Characterization of Serratia liquefaciens Associated with Rot Disease of Malus domestica (Apple) Fruit and Its Inhibition by Origanum vulgare (Oregano) Oil

Khalifa

2022

Horticulturae

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Microbial food spoilage has been a major source of concern because it widens the global food production-to-consumption gap. Thus, in the current study, we aimed to isolate, characterize and identify bacteria associated with spoiled apple fruit in Al-Ahsa city, Saudi Arabia. MDK33, a bacterial strain isolated from rotten apple fruit, was characterized at the phenotypic and genotypic levels. Furthermore, the antibacterial effect of oregano essential oil (OEO) against MDK33 was investigated. MDK33 formed circular colonies with entire margins, and the cells were Gram-negative rods with no endospores. Biochemical characterizations of MDK33, as revealed by the Biolog Gen III MicroPlate test system, indicated that the strain utilized 66 (~70%) of the 94 diverse metabolites and did not utilize 29 (~30%). MDK33 grew well on a variety of substrates, including pectin, gelatin, Tween 40 and dextrin, at pH 5 and 6; tolerated salts up to 4% NaCl; and was resistant to multiple antibiotics. Furthermore, based on the sequences of the 16S rRNA gene, MDK33 was identified as Serratia liquefaciens at a 99.73% identity level. Koch’s postulates were affirmed, confirming that S. liquefaciens MDK33 is the causative agent of apple rot disease in Al-Ahsa, Saudi Arabia. The minimum inhibitory concentration (MIC) of 0.23 mg/mL for oregano oil against S. liquefaciens MDK33 demonstrated remarkable antibacterial and antibiofilm activities. This is the first isolation of pathogenic S. liquefaciens as the causative agent of rot disease of apple fruit and its inhibition by oregano oil. Furthermore, the findings pave the way for oregano oil to be evaluated as a natural preservative to reduce post-harvest losses while maintaining high quality for sustainable food security in future studies.

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Section: Discussionsupporting

confidence: 91%

First Isolation and Characterization of Serratia liquefaciens Associated with Rot Disease of Malus domestica (Apple) Fruit and Its Inhibition by Origanum vulgare (Oregano) Oil

Khalifa

2022

Horticulturae

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“…A previously reported larval package test (LPT) [ 28 , 29 ] was used to measure the entomopathogenic effect on cattle ticks. This test was utilized to assess the pathogenic activity of the bacterial cultures of the S. shinii strain 1 (S-1) (INIFAP 005-008), S. shinii strain 2 (S-2) (INIFAP 004-015), S. xylosus (INIFAP 009-16), and S. succinus (INIFAP 002-2015) (the bacterial species used are listed in Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Efficacy of Entomopathogenic Staphylococcus Bacteria as a Biocontrol Agent against Rhipicephalus microplus Ticks: Assessing Reproductive Inhibition and Mortality Rates

Cossio-Bayugar,

Arreguin-Perez,

Aguilar-Diaz

et al. 2024

Microorganisms

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Rhipicephalus microplus is a persistent ectoparasite of cattle that causes bovine anaplasmosis and babesiosis, causing economic losses worldwide. Chemical treatment is the primary method for tick control, but the emergence of pesticide-resistant ticks is a major challenge. Alternative biocontrol strategies utilizing entomopathogenic microorganisms are being explored. This study aimed to validate the species identification and assess the efficacy of four strains of Staphylococcus bacteria (S. shinii S1 and S-2, S. succinus, and S. xylosus) previously reported as being entomopathogenic to R. microplus ticks. According to the bioassays, S. shinii S-1 exhibited the greatest degree of reproductive inhibition (47%), followed by S. succinus (44.3%) at a concentration of 1 × 108 cfu/mL. S. xylosus displayed decreased reproductive inhibition (6.3%). In an additional bioassay, S. shinii S-1 exhibited a significant larval mortality of 67.63%, followed by S. succinus with 66.75%, S. shinni S-2 with 64.61%, and S. xylosus with 28.18% mortality. The common signs of infection observed on these ticks included swelling, yellowish exudate on the hypostome, and reduced limb mobility and color change, except for S. succinus, which did not cause color changes. These bacteria were naturally found on bovine skin. However, further studies are needed to confirm their potential as promising alternatives or complementary agents to existing acaricidal compounds.

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Histometric and morphological damage caused by Serratia marcescens to the tick Rhipicephalus microplus (Acari: Ixodidae)

Castro-Saines

Peña-Chora²,

Hallal-Calleros³

et al. 2022

Arch Microbiol

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Characterization of a strain of Serratia sp. with ixodicide activity against the cattle tick Rhipicephalus microplus

Cited by 5 publications

References 37 publications

First Isolation and Characterization of Serratia liquefaciens Associated with Rot Disease of Malus domestica (Apple) Fruit and Its Inhibition by Origanum vulgare (Oregano) Oil

First Isolation and Characterization of Serratia liquefaciens Associated with Rot Disease of Malus domestica (Apple) Fruit and Its Inhibition by Origanum vulgare (Oregano) Oil

Efficacy of Entomopathogenic Staphylococcus Bacteria as a Biocontrol Agent against Rhipicephalus microplus Ticks: Assessing Reproductive Inhibition and Mortality Rates

Histometric and morphological damage caused by Serratia marcescens to the tick Rhipicephalus microplus (Acari: Ixodidae)

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