2004
DOI: 10.1007/s00438-004-1068-7
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Characterization of a T-DNA insertion mutant for the protein import receptor atToc33 from chloroplasts

Abstract: In Arabidopsis thaliana, the Toc34 receptor component of the chloroplast import machinery is encoded by two independent but highly homologous genes, atToc33 and atToc34. We have isolated a T-DNA insertion mutant of atToc33 which is characterized by a pale phenotype, due to reductions in the levels of photosynthetic pigments, and alterations in protein composition. The latter involve not only chloroplast proteins but also some cytosolic polypeptides, including 14-3-3 proteins which, among other functions, have … Show more

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Cited by 28 publications
(30 citation statements)
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“…Seeds for oastlB (N521183) and oastlC (N500860) plants were obtained from the SALK collection (Salk Institute Genomic Analysis Laboratory). The oastlA mutant, derived from the T. Jack collection (Campisi et al, 1999), was isolated out of a pool of 10 plants (N19847) from the screening for the atToc33 mutant (Gutensohn et al, 2004) and then backcrossed with Col-0. All seeds were verified for homozygosity of the corresponding T-DNA insertion.…”
Section: Plant Genotypes and Growth Conditionsmentioning
confidence: 99%
“…Seeds for oastlB (N521183) and oastlC (N500860) plants were obtained from the SALK collection (Salk Institute Genomic Analysis Laboratory). The oastlA mutant, derived from the T. Jack collection (Campisi et al, 1999), was isolated out of a pool of 10 plants (N19847) from the screening for the atToc33 mutant (Gutensohn et al, 2004) and then backcrossed with Col-0. All seeds were verified for homozygosity of the corresponding T-DNA insertion.…”
Section: Plant Genotypes and Growth Conditionsmentioning
confidence: 99%
“…The leaf material was ground in 750 ll acetone with barium carbonate and centrifuged twice to remove cell fragments. The supernatant was used for further analysis and pigments were separated by HPLC as described (Gutensohn et al, 2004). The pigment content of wild-type seedlings was taken as 100% and the chlorophyll content of mutant seedlings expressed as % of wild type.…”
Section: Pigment Analysismentioning
confidence: 99%
“…These mutations are different from those impeding chloroplast development not only in cotyledons but also in true leaves and that give rise to various albino, xantha, and yellow green mutants (Runge et al 1995;Sundberg et al 1997). The identification of these latter mutated genes led to the identification of components of the protein import machinery of chloroplasts, enzymes of chlorophyll biosynthesis and factors needed for the assembly of photosynthetic membranes (Asano et al 2004;Chou et al 2003;Gutensohn et al 2004;Gutierrez-Nava Mde et al 2004).…”
Section: Introductionmentioning
confidence: 96%