Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

Liao, Yen‐Te; Zhang, Yujie; Salvador, A.; Harden, Leslie A.; Wu, Vivian C.H.

doi:10.1128/spectrum.02220-21

Cited by 24 publications

(31 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in the absence of experimental data, including this feature would have increased the model’s complexity without providing any real benefit to the present study. However, the increasing application of phages for eco-restoration ( 57 ), food safety ( 58 , 59 ), and sterilization of surfaces ( 60 – 64 ) implies that phage-derived antibacterial treatments need to work even in the absence of a complementary immune response.…”

Section: Discussionmentioning

confidence: 99%

Determination of phage load and administration time in simulated occurrences of antibacterial treatments

et al. 2022

View full text Add to dashboard Cite

The use of phages as antibacterials is becoming more and more common in Western countries. However, a successful phage-derived antibacterial treatment needs to account for additional features such as the loss of infective virions and the multiplication of the hosts. The parameters critical inoculation size (VF) and failure threshold time (TF) have been introduced to assure that the viral dose (Vϕ) and administration time (Tϕ) would lead to the extinction of the targeted bacteria. The problem with the definition of VF and TF is that they are non-linear equations with two unknowns; thus, obtaining their explicit values is cumbersome and not unique. The current study used machine learning to determine VF and TF for an effective antibacterial treatment. Within these ranges, a Pareto optimal solution of a multi-criterial optimization problem (MCOP) provided a pair of Vϕ and Tϕ to facilitate the user’s work. The algorithm was tested on a series of in silico microbial consortia that described the outgrowth of a species at high cell density by another species initially present at low concentration. The results demonstrated that the MCOP-derived pairs of Vϕ and Tϕ could effectively wipe out the bacterial target within the context of the simulation. The present study also introduced the concept of mediated phage therapy, where targeting booster bacteria might decrease the virulence of a pathogen immune to phagial infection and highlighted the importance of microbial competition in attaining a successful antibacterial treatment. In summary, the present work developed a novel method for investigating phage/bacteria interactions that can help increase the effectiveness of the application of phages as antibacterials and ease the work of microbiologists.

show abstract

Section: Discussionmentioning

confidence: 99%

Determination of phage load and administration time in simulated occurrences of antibacterial treatments

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Phage Sa157lw was purified through a CsCl gradient and subsequently subjected to DNA extraction and library preparation before sequencing as previously described ( Liao et al, 2022 ). Later, the samples were loaded to a MiSeq Reagent Kit v2 (500-cycle) and sequenced on the MiSeq platform (Illumina, San Deigo, CA, USA), generating approximately 14 million 2 × 250-bp paired-end reads.…”

Section: Methodsmentioning

confidence: 99%

“…One-step growth curve of phage Sa157lw was conducted separately on E. coli O157:H7 (ATCC 43888) and Salmonella Typhimurium (ATCC 14028) based on the previous method with subtle modification ( Liao et al, 2022 ). In brief, the fresh culture of each bacterial strain was prepared in 10 ml of TSB at 37°C for 20 h; 0.2 ml of the overnight culture was sub-cultured in 19.8 ml of TSB and incubated for 2 h at 37°C to reach the log phase of the bacterial growth.…”

Section: Methodsmentioning

confidence: 99%

“…A batch of mung beans seeds obtained from a local store was disinfected by 2% of NaClO solution for 15 min, followed by rinsing with sterile water five times and air drying at room temperature under a biosafety hood as previously described (Liao et al, 2022). Later, 5 g of the sterile mung bean seeds were homogenized with 20 ml 0.1% peptone water and plated on Sorbitol MacConkey (SMAC; Oxoid, Basingstoke, United Kingdom) and xylose lysine deoxycholate (XLD; Oxoid, Basingstoke, United Kingdom) plates to check if the seeds were free of E. coli and Salmonella.…”

Section: Phage Application On Mung Bean Seedsmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of polyvalent Escherichia phage Sa157lw for the biocontrol potential of Salmonella Typhimurium and Escherichia coli O157:H7 on contaminated mung bean seeds

Liao

Zhang²,

Salvador³

et al. 2022

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Seeds are one of the primary sources of contamination with foodborne pathogens, such as pathogenic Escherichia coli, and various Salmonella serovars, for produce, particularly sprouts. Due to the susceptibility of sprout growth to chemical-based antimicrobials and the rising issue of antimicrobial resistance, developing innovative antimicrobial interventions is an urgent need. Therefore, the objective of this study was to characterize Escherichia phage Sa157lw (or Sa157lw) for the biocontrol potential of Salmonella Typhimurium and E. coli O157:H7 on contaminated mung bean seeds. Phage Sa157lw was subjected to whole-genome sequencing and biological characterization, including morphology, one-step growth curve, and stress stability tests. Later, antimicrobial activity was determined in vitro and upon application on the mung bean seeds artificially contaminated with E. coli O157:H7 or Salmonella Typhimurium. Sa157lw possessed a contractile tail and belonged to the Kuttervirus genus under the Ackermannviridae family, sharing a close evolutionary relationship with E. coli phage ECML-4 and Kuttervirus ViI; however, tail spike genes (ORF_102 and ORF_104) were the primary region of difference. Comparative genomics showed that Sa157lw encoded a cluster of tail spike genes—including ORF_101, ORF_102, and ORF_104—sharing high amino acid similarity with the counterfeits of various Salmonella phages. Additionally, Sa157lw harbored a unique tail fiber (ORF_103), possibly related to the receptors binding of O157 strains. The genomic evidence accounted for the polyvalent effects of Sa157lw against E. coli O157:H7 and various Salmonella serovars (Typhimurium, Enteritidis, Agona, Saintpaul, and Heidelberg). Furthermore, the phage did not contain any virulence, antibiotic-resistant, or lysogenic genes. Sa157lw had a 30-min latent period on both E. coli O157:H7 and Salmonella Typhimurium, with an estimated burst size of 130 and 220 PFU/CFU, respectively, and was stable at a wide range of temperatures (4–60°C) and pH (pH4 to pH10). The phage application demonstrated a strong anti-E. coli O157:H7 and anti-Salmonella Typhimurium effects in 1.1 and 1.8 log reduction on the contaminated mung bean seeds after overnight storage at 22°C. These findings provide valuable insights into the polyvalent Sa157lw as a potential biocontrol agent of Salmonella Typhimurium and E. coli O157:H7 on sprout seeds.

show abstract

“…Bacteriophages can be directly utilized on solid food surfaces via different methods, including brushing, dipping or spraying. They have been utilized as antimicrobial agents against STEC in a variety of food products, such as milk and meat (Li et al, 2021;Tomat et al, 2018), mung bean seeds (Liao, Zhang, Salvador, Harden, & Wu, 2022), romaine lettuce (Park et al, 2020) and burger patties . However, the direct addition techniques may have a negative impact on the antimicrobial activity of bacteriophages because of their low stability in food environments and uncontrolled antimicrobial release (Alves et al, 2019;Costa et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Development of antimicrobial composite materials for food packaging applications

Choo¹

View full text Add to dashboard Cite

Due to increasing environmental concerns regarding plastic waste, the development of composite film materials from biodegradable polymers and natural food additives is gaining more attention. Over the last few decades, the safety of synthetic additives that are commonly added as food additives has also been questioned because of their potential toxicity, carcinogenicity and teratogenicity effects. Natural antimicrobial agent could be a promising and versatile component in food packaging materials to improve their physiochemical, antioxidant and antimicrobial properties and hence, improve food quality and shelf life. In this study, novel chitosan/acetylated starch and polyvinyl alcohol/chitosan composite films were developed via a solution casting technique. In addition, a novel Escherichia phage, CAM-21, was isolated from a slurry lagoon at a local dairy farm before incorporating into soy protein isolate-based films. The results indicate that the chitosan/acetylated starch films incorporated with cinnamon and clove essential oils displayed enhanced light, water vapor, and oxygen barrier characteristics, as well as excellent antibacterial activity against spoilage bacteria and the pathogen, Escherichia coli O157:H7, on beef. Polyvinyl alcohol/chitosan films incorporated with aminosilane-modified bacterial nanocellulose and 4-hexylresorcinol exhibited improved mechanical, water vapor barrier, antioxidant, and antimicrobial properties against spoilage bacteria on vacuum packaged refrigerated raw beef. Also, CAM-21 has a broad host spectrum against various serotypes of Shiga toxin-producing E. coli. The growth of E. coli O157:H7 was effectively controlled in phage-treated milk, ground beef and baby spinach. Soy protein isolate films incorporated with CAM-21 showed excellent antimicrobial activity against E. coli O157:H7 in broth and refrigerated beef products. This project demonstrates the potential application of natural antimicrobial agents to produce novel composite films for antimicrobial food packaging.

show abstract

Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

Cited by 24 publications

References 78 publications

Determination of phage load and administration time in simulated occurrences of antibacterial treatments

Determination of phage load and administration time in simulated occurrences of antibacterial treatments

Characterization of polyvalent Escherichia phage Sa157lw for the biocontrol potential of Salmonella Typhimurium and Escherichia coli O157:H7 on contaminated mung bean seeds

Development of antimicrobial composite materials for food packaging applications

Contact Info

Product

Resources

About