Characterization of a temperature sensitive feline infectious peritonitis coronavirus

Christianson, K K; Ingersoll, J D; Landon, R. M.; Pfeiffer, N. E.; Gerber, J D

doi:10.1007/bf01311080

Cited by 32 publications

(22 citation statements)

References 17 publications

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“…Previous reports on the plaque characteristic in relation to both serotypes and disease potential [2,3,16,28] are in somewhat conflict with each other as well as with the present results. Highly virulent FIPV strains NOR15 and 79-1146 produced larger homogenous plaques in comparison to FECV 79-1683 and CCV 1-71 strains which produced a heterogenous population of plaque sizes [16,28].…”

Section: Discussioncontrasting

confidence: 99%

Antigenic and Plaque Variations of Serotype II Feline Infectious Peritonitis Coronaviruses.

et al. 1997

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ABSTRACT. Three feline coronavirus (FCoV) isolates KUK-H, M91-266, and M91-267 were examined to elucidate their biological and antigenic properties as well as disease potential in cats. Immune stainings of virus-infected cells by using FCoV type-specific monoclonal antibodies indicated that their antigenic specificity was serotype II. However, antigenic variations among these serotype II FCoVs were detected by neutralization assay with hyperimmune antisera against FCoVs and canine coronaviruses, and with experimentally infected cat sera; there were two subtypes in serotype II FCoVs. The isolates efficiently grew in fcwf-4 cell culture showing lytic CPE enough to form distinct plaques; when measured 48 hr after infection, plaque sizes of both M91-266 and M91-267 were approximately 1 mm in diameter, and a mixture of small (less than 1mm in diameter) and large (approximately 3 mm in diameter) plaques were produced in the case of KUK-H. Strains KUK-H, M91-266 and M91-267 produced feline infectious peritonitis (FIP) in 50%, 67% and 89% of experimentally inoculated kittens, respectively. Furthermore, 80% of the kittens inoculated with the small plaque former of KUK-H developed FIP accompanied by more prominent clinical signs as well as pathological changes when compared with 28.6% of kittens inoculated with the large plaque former. These results suggest that serotype II FIPVs producing smaller size of plaques are more virulent than those producing larger size of plaques. -KEY WORDS: coronavirus, feline, feline infectious peritonitis, plaque, virulence.J. Vet. Med. Sci. 59(4): 253-258, 1997 serotype II FCoV has arisen by recombination between serotype I FCoV and CCV. In the present paper, three serotype II FCoV isolates from clinical FIP cases were examined in respects of antigenic property and in vivo pathogenic virulence especially in relation to plaque characteristics. Discussion was also made regarding relationships between plaque properties and virulence of coronaviruses in animals. MATERIALS AND METHODSViruses and cell culture: KUK-H strain was isolated in 1987 by using CRFK cells (ATCC CCL94), and both M91-266 and M91-267 strains were isolated in 1991 by using fcwf-4 cells. All derived from the spleen samples taken at the postmortem examination of effusive form FIP field cases. Stock viruses were prepared after further several passages in fcwf-4 cell culture. Strain C3663, a local isolate from an effusive form FIP case, was used as a reference serotype I FCoV. The cells were cultured as described previously [18].Plaque assay: The stock viruses were characterized by plaque assay, and then the virus was plaque-purified for further experiments. After serial tenfold virus dilutions, 0.2 ml of each dilution was inoculated onto fcwf-4 cell monolayer in 60-mm plastic plates. The plates were incubated at 37°C for 1 hr, and 4 ml of an overlay medium was added. The plates were further incubated at 37°C for 2 or 3 days and then stained with either an overlay medium According to the recent concept about feline coronaviru...

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Section: Discussioncontrasting

confidence: 99%

Antigenic and Plaque Variations of Serotype II Feline Infectious Peritonitis Coronaviruses.

et al. 1997

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“…Nevertheless, a vaccine was licensed (Primucell, Pfizer Animal Health) incorporating a temperature-sensitive mutant of the FCoV strain DF2-FIPV, which can replicate in the cool lining of the upper respiratory tract but not at higher internal body temperatures [172][173][174][175]. This vaccine, administered intranasally, produces local immunity (IgA antibodies) at the site where FCoV first enters the body (the oropharynx) and also induces cell-mediated immunity.…”

Section: Vaccinationmentioning

confidence: 99%

Feline infectious peritonitis

Hartmann

2005

Veterinary Clinics of North America: Small Animal Practice

148

251

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The article discusses feline infectious peritonitis (FIP), an important disease frequently seen in veterinary practice. FIP causes many problems to the veterinarian as it can be difficult to definitively diagnose the disease, as there is no effective treatment, and as prophylactic interventions are not very successful. Although intense research has created a lot of new knowledge about this disease in the last years, there are still many unanswered questions. The objective of this article is to review recent knowledge and to increase understanding of the complex pathogenesis of FIP.

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“…Although FCoV has been known for more than 40 years to be the causative pathogen of FIP, the immunopathogenic nature of the disease has prevented the success of vaccination trials (Christianson et al, 1989;Scott, 1987;Stoddart et al, 1988;Vennema et al, 1990a,b). Several studies have attempted to identify effective anti-FCoV treatments for FIP-diseased cats (Hartmann and Ritz, 2008).…”

Section: Introductionmentioning

confidence: 99%

Synergistic antiviral effect of Galanthus nivalis agglutinin and nelfinavir against feline coronavirus

Hsieh

Lin

et al. 2010

Antiviral Research

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Feline infectious peritonitis (FIP) is a fatal disease in domestic and nondomestic felids caused by feline coronavirus (FCoV). Currently, no effective vaccine is available for the prevention of this disease. In searching for agents that may prove clinically effective against FCoV infection, 16 compounds were screened for their antiviral activity against a local FCoV strain in Felis catus whole fetus-4 cells. The results showed that Galanthus nivalis agglutinin (GNA) and nelfinavir effectively inhibited FCoV replication. When the amount of virus preinoculated into the test cells was increased to mimic the high viral load present in the target cells of FIP cats, GNA and nelfinavir by themselves lost their inhibitory effect. However, when the two agents were added together to FCoV-infected cells, a synergistic antiviral effect defined by complete blockage of viral replication was observed. These results suggest that the combined use of GNA and nelfinavir has therapeutic potential in the prophylaxis and treatment of cats with early-diagnosed FIP.

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Characterization of a temperature sensitive feline infectious peritonitis coronavirus

Cited by 32 publications

References 17 publications

Antigenic and Plaque Variations of Serotype II Feline Infectious Peritonitis Coronaviruses.

Antigenic and Plaque Variations of Serotype II Feline Infectious Peritonitis Coronaviruses.

Feline infectious peritonitis

Synergistic antiviral effect of Galanthus nivalis agglutinin and nelfinavir against feline coronavirus

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