Characterization of a thermophilic facultatively anaerobic bacterium Paenibacillus sp. strain DA-C8 that exhibits xylan degradation under anaerobic conditions

Chhe, Chinda; Uke, Ayaka; Baramee, Sirilak; Tachaapaikoon, Chakrit; Pason, Patthra; Waeonukul, Rattiya; Kosugi, Akihiko

doi:10.1016/j.jbiotec.2021.10.008

Cited by 5 publications

(10 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Hungate technique for enrichment culturing was used to isolate xylanolytic anaerobic microbes. The basal medium purged with nitrogen (BMN) was composed of (per litre): 2.9 g K 2 HPO 4 , 4.2 g urea, 1.0 g Na 2 CO 3 , 2.0 g yeast extract, 0.5 g l -cysteine-HCl·H 2 O, 0.01 g CaCl 2 ·2H 2 O, 0.5 mg resazurin and 200 µl mineral solution (25.0 g/L MgCl 2 ·6H 2 O, 0.312 g/L FeSO 4 ·7H 2 O and 37.5 g/L CaCl 2 ·2H 2 O), as described previously [12]. All chemicals were purchased from Fujifilm Wako Pure Chemical Corporation.…”

Section: Isolation and Ecologymentioning

confidence: 99%

“…Following this approach, the strain DA-C8 T was successfully isolated as a pure culture. Strain DA-C8 T could completely degrade 1 % beech wood xylan under anaerobic thermophilic (55 °C) conditions in 4 days [12].…”

Section: Isolation and Ecologymentioning

confidence: 99%

“…Catalase and oxidase tests were carried out using a Catalase Assay Kit (Megazyme) and Cytochrome Oxidase Test Strips (Nissui), respectively. Strain DA-C8 T was catalase-positive and oxidase-negative; however, the catalase activity of strain DA-C8 T grown under aerobic growth conditions was low [12]. Strain DA-C8 T was able to use the following carbon sources (1 % w/v): glucose, galactose, xylose, arabinose, fructose, mannose, raffinose, cellobiose, maltose, sucrose, ribose, xylan, wheat arabinoxylan, galactan, pectin and aesculin ().…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…Interestingly, the xylan degradation ability of strain DA-C8 T , as well as its growth rate and cell density, decreased under aerobic culture conditions compared with that under anaerobic conditions [12]. Repression of xylan-degrading enzymes was observed in the aerobic culture of strain DA-C8 T [12].…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

See 3 more Smart Citations

Insulambacter thermoxylanivorax sp. nov., a thermophilic xylanolytic bacterium isolated from compost

Chhe

Uke

Baramee

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

We isolated and analysed a Gram-negative, facultatively thermophilic, xylan-degrading bacterium that we designated as strain DA-C8T. The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source. The strain showed high xylan degradation ability under anaerobic growth conditions. The isolate grew at 37–60 °C (optimum, 55 °C) and pH 4.0–11.0 (optimum, pH 9.0). As well as xylan, strain DA-C8T could use polysaccharides such as arabinoxylan and galactan as carbon sources. Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %). In phylogenetic analysis, strain DA-C8T belonged to the same lineage as Xylanibacillus composti K13T (92.5 %), but there was less statistical support for branching (70 %). Digital DNA–DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively. Those between strain DA-C8T and X. composti K13 were 23.7, 67.7 and 57.6 %, respectively. The whole-genome DNA G+C content of strain DA-C8T was 52.3 mol%. The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed—Insulambacter gen. nov.—for the novel species Insulambacter thermoxylanivorax sp. nov. The type strain is DA-C8T (=JCM 34211T=DSM 111723T).

show abstract

Section: Isolation and Ecologymentioning

confidence: 99%

Section: Isolation and Ecologymentioning

confidence: 99%

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

See 2 more Smart Citations

Insulambacter thermoxylanivorax sp. nov., a thermophilic xylanolytic bacterium isolated from compost

Chhe

Uke

Baramee

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…LJ-23. In addition, the genus Paenibacillus has been reported to show excellent capacity at degrading a variety of polysaccharides with complex structures, including chitin, xylan, cellulose, alginate, and even the capsular polysaccharide from bacteria [16,[20][21][22][23][24][25]. However, the gene encoding alginate lyase from the genus Paenibacillus has rarely been identified.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of a Novel Alginate Lyase from Paenibacillus sp. LJ-23

et al. 2022

View full text Add to dashboard Cite

As a low molecular weight alginate, alginate oligosaccharides (AOS) exhibit improved water solubility, better bioavailability, and comprehensive health benefits. In addition, their biocompatibility, biodegradability, non-toxicity, non-immunogenicity, and gelling capability make them an excellent biomaterial with a dual curative effect when applied in a drug delivery system. In this paper, a novel alginate lyase, Algpt, was cloned and characterized from a marine bacterium, Paenibacillus sp. LJ-23. The purified enzyme was composed of 387 amino acid residues, and had a molecular weight of 42.8 kDa. The optimal pH of Algpt was 7.0 and the optimal temperature was 45 °C. The analysis of the conserved domain and the prediction of the three-dimensional structure indicated that Algpt was a novel alginate lyase. The dominant degradation products of Algpt on alginate were AOS dimer to octamer, depending on the incubation time, which demonstrated that Algpt degraded alginate in an endolytic manner. In addition, Algpt was a salt-independent and thermo-tolerant alginate lyase. Its high stability and wide adaptability endow Algpt with great application potential for the efficient preparation of AOS with different sizes and AOS-based products.

show abstract

Genomic analysis of Paenibacillus macerans strain I6, which can effectively saccharify oil palm empty fruit bunches under nutrient-free conditions

Uke

Sornyotha

Baramee

et al. 2023

Journal of Bioscience and Bioengineering

View full text Add to dashboard Cite

Characterization of a thermophilic facultatively anaerobic bacterium Paenibacillus sp. strain DA-C8 that exhibits xylan degradation under anaerobic conditions

Cited by 5 publications

References 35 publications

Insulambacter thermoxylanivorax sp. nov., a thermophilic xylanolytic bacterium isolated from compost

Insulambacter thermoxylanivorax sp. nov., a thermophilic xylanolytic bacterium isolated from compost

Cloning and Characterization of a Novel Alginate Lyase from Paenibacillus sp. LJ-23

Genomic analysis of Paenibacillus macerans strain I6, which can effectively saccharify oil palm empty fruit bunches under nutrient-free conditions

Contact Info

Product

Resources

About