Characterization of a thermostable glucose isomerase with an acidic pH optimum from Acidothermus cellulolyticus

Characterization of a d-psicose 3-epimerase from Dorea sp. CAG317 with an acidic pH optimum and a high specific activity

“…In past years, molecular modification using modern genetic and protein engineering has been widely used in d-glucose isomerase and l-arabinose isomerase researches for improving the thermostability or shifting pH optimum toward acidic side [19,20], and some ideal enzymes have also been characterized from the thermophilic or acidophilic bacteria [21,22]. By comparison, the related studies on ketose 3-epimerases are relatively few, especially the acidic pH optimum.…”

Production of d‐mannose from d‐glucose by co‐expression of d‐glucose isomerase and d‐lyxose isomerase in Escherichia coli

“…Co 2+ was the optimum metal cofactor for the co‐expression system, but for Acce‐GI and Thoc‐LI the optimum metal cofactors were Mg 2+ and Mn 2+ respectively. As previously reported, both Acce‐GI and Thoc‐LI were metal‐independent enzymes, but their enzyme activities can be enhanced by supplementation of some divalent metal . For free Acce‐GI, the use of Mg 2+ , Co 2+ , and Ni 2+ improves the enzyme activity, while Zn 2+ inhibits the activity.…”

“…The pH experiments were determined in three different buffer systems (50 mmol L −1 ), containing acetate buffer (pH 5.0–6.0), phosphate buffer (pH 6.0–7.0) and Tris–HCl buffer (pH 7.0–8.0). The co‐expression system exhibited the maximum activity at a weak acidic pH (pH 6.5), which was the same as that of free Acce‐GI and Thoc‐LI (pH 6.5) . The co‐expression system displayed high relative activities in the weak acidic pH conditions.…”

“…In previous work, we have overexpressed and characterized the d ‐GI from Acidothermus cellulolyticus 11B (Acce‐GI), and d ‐LI from Thermosediminibacter oceani DSM 16646 (Thoc‐LI) . Both of the isomerases are relatively thermostable and weak‐acid stable.…”

Isolation, Screening and Determination of Α-Amylase Activity From Marine Streptomyces Species

“…However, the distribution of Streptomyces in the marine is chiefly unexplored or underexploited habitats be pursued as sources of novel compounds for industrial usage [28]. Mangrove is a high moisture, high salinity and hypoxia to tolerant ecosystem [29] which breeds many kinds of novel microorganisms which are the main source of vital compounds [30]. Many researchers investigated the muthupet mangrove vegetation, isolation and characterization of bioactive compounds from Streptomyces [31][32].…”