Characterization of a Tomato Xyloglucan Endotransglycosylase Gene That Is Down-Regulated by Auxin in Etiolated Hypocotyls

Catalá, Carmen; Rose, Jocelyn K. C.; York, William S.; Albersheim, Peter; Darvill, Alan G.; Bennett, A. B.

doi:10.1104/pp.010481

Cited by 80 publications

(39 citation statements)

References 58 publications

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“…Previous work indicates that increased IAA content can be coupled to regulation of XTH and the increased deposition of lignin, as was observed in the transgenic lines [63-65]. Catalá et al [65] reported a down-regulation of a tomato XTH gene ( LeXET2 ) by auxin and transgenic tobacco lines overproducing IAA exhibited increased lignin content and altered lignin composition [63]. Sitbon et al [64] suggested that the increased lignin deposition may have resulted from increased peroxidase activity, brought about by increased IAA levels and in the transgenic lines, expression levels of several class III peroxidases changed.…”

Section: Resultsmentioning

confidence: 83%

“…Auxin, for example, has an effect on cell wall structure and expansion, acting in combination with brassinosteroids, which was the only other hormone-related GO category enriched in the transgenic lines. Previous work indicates that increased IAA content can be coupled to regulation of XTH and the increased deposition of lignin, as was observed in the transgenic lines [63-65]. Catalá et al [65] reported a down-regulation of a tomato XTH gene ( LeXET2 ) by auxin and transgenic tobacco lines overproducing IAA exhibited increased lignin content and altered lignin composition [63].…”

Section: Resultsmentioning

confidence: 98%

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Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

et al. 2011

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BackgroundPolygalacturonase-inhibiting proteins (PGIPs) directly limit the effective ingress of fungal pathogens by inhibiting cell wall-degrading endopolygalacturonases (ePGs). Transgenic tobacco plants over-expressing grapevine (Vitis vinifera) Vvpgip1 have previously been shown to be resistant to Botrytis infection. In this study we characterized two of these PGIP over-expressing lines with known resistance phenotypes by gene expression and hormone profiling in the absence of pathogen infection.ResultsGlobal gene expression was performed by a cross-species microarray approach using a potato cDNA microarray. The degree of potential cross-hybridization between probes was modeled by a novel computational workflow designed in-house. Probe annotations were updated by predicting probe-to-transcript hybridizations and combining information derived from other plant species. Comparing uninfected Vvpgip1-overexpressing lines to wild-type (WT), 318 probes showed significant change in expression. Functional groups of genes involved in metabolism and associated to the cell wall were identified and consequent cell wall analysis revealed increased lignin-levels in the transgenic lines, but no major differences in cell wall-derived polysaccharides. GO enrichment analysis also identified genes responsive to auxin, which was supported by elevated indole-acetic acid (IAA) levels in the transgenic lines. Finally, a down-regulation of xyloglucan endotransglycosylase/hydrolases (XTHs), which are important in cell wall remodeling, was linked to a decrease in total XTH activity.ConclusionsThis evaluation of PGIP over-expressing plants performed under pathogen-free conditions to exclude the classical PGIP-ePG inhibition interaction indicates additional roles for PGIPs beyond the inhibition of ePGs.

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Section: Resultsmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 98%

Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

et al. 2011

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“…XTH genes constitute a multigene family encoding proteins involved in cell wall construction and disassembly, with diverse levels of expression in Arabidopsis thaliana [38]. In tomato, two isozymes of XTH (LeXET and LeXET2) with opposite patterns of expression have been detected [39]. LeXET was abundant in the rapid elongation region, whereas LeXET2 was more abundant in the mature/non elongating region.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling of bud dormancy induction and release in oak by next-generation sequencing

Ueno

Klopp²,

Leplé³

et al. 2013

BMC Genomics

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BackgroundIn temperate regions, the time lag between vegetative bud burst and bud set determines the duration of the growing season of trees (i.e. the duration of wood biomass production). Dormancy, the period during which the plant is not growing, allows trees to avoid cold injury resulting from exposure to low temperatures. An understanding of the molecular machinery controlling the shift between these two phenological states is of key importance in the context of climatic change. The objective of this study was to identify genes upregulated during endo- and ecodormancy, the two main stages of bud dormancy. Sessile oak is a widely distributed European white oak species. A forcing test on young trees was first carried out to identify the period most likely to correspond to these two stages. Total RNA was then extracted from apical buds displaying endo- and ecodormancy. This RNA was used for the generation of cDNA libraries, and in-depth transcriptome characterization was performed with 454 FLX pyrosequencing technology.ResultsPyrosequencing produced a total of 495,915 reads. The data were cleaned, duplicated reads removed, and sequences were mapped onto the oak UniGene data. Digital gene expression analysis was performed, with both R statistics and the R-Bioconductor packages (edgeR and DESeq), on 6,471 contigs with read numbers ≥ 5 within any contigs. The number of sequences displaying significant differences in expression level (read abundance) between endo- and ecodormancy conditions ranged from 75 to 161, depending on the algorithm used. 13 genes displaying significant differences between conditions were selected for further analysis, and 11 of these genes, including those for glutathione-S-transferase (GST) and dehydrin xero2 (XERO2) were validated by quantitative PCR.ConclusionsThe identification and functional annotation of differentially expressed genes involved in the “response to abscisic acid”, “response to cold stress” and “response to oxidative stress” categories constitutes a major step towards characterization of the molecular network underlying vegetative bud dormancy, an important life history trait of long-lived organisms.

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“…The corresponding genes have been cloned and expressed in Escherichia coli where the heterologous protein generally appears as inclusion bodies (6,(14)(15)(16). One XET isoenzyme from Arabidopsis has been successfully expressed in insect cells using the baculoviral vectors (17) and an isoenzyme of XET from tomato has been expressed in the methylotrophic yeast, Pichia pastoris (18). However, the enzyme yields in all published cases have been relatively low and no attempts have been made to optimize the enzyme production for enhanced yields.…”

Section: Introductionmentioning

confidence: 98%

Production of poplar xyloglucan endotransglycosylase using the methylotrophic yeast Pichia pastoris

Bollok

Henriksson

Kallas

et al. 2005

Appl Biochem Biotechnol

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The gene XET16A encoding the enzyme xyloglucan endotransglycosylase (XET) from hybrid aspen (Populus tremula x tremuloides Mich) was transformed into Pichia pastoris GS115 and the enzyme was secreted to the medium. The influence of process conditions on the XET production, activity, and proteolytic degradation were examined. Inactivation of XET occurred in the foam, but could be decreased significantly by using an efficient antifoam. Rich medium (yeast extract plus peptone) was needed for product accumulation, but not for growth. The proteolytic degradation of the enzyme in the medium was substantially decreased by also adding yeast extract and peptone to the glycerol medium before induction with methanol. Decreasing the fermentation pH from 5.0 to 4.0 further reduced the proteolysis. The specific activity was further improved by production at 15 degrees C instead of 22 degrees C. In this way a XET production of 54 mg/L active enzyme could be achieved in the process with a specific activity of 18 Unit/mg protein after a downstream process including centrifugation, micro- and ultrafiltration, and ion exchange chromatography.

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Characterization of a Tomato Xyloglucan Endotransglycosylase Gene That Is Down-Regulated by Auxin in Etiolated Hypocotyls

Cited by 80 publications

References 58 publications

Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

Transcriptional profiling of bud dormancy induction and release in oak by next-generation sequencing

Production of poplar xyloglucan endotransglycosylase using the methylotrophic yeast Pichia pastoris

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