Characterization of a two-component regulatory system from Acinetobacter baumannii that controls biofilm formation and cellular morphology

Tomaras, Andrew P.; Flagler, Michael J.; Dorsey, Caleb W.; Gaddy, Jennifer A.; Actis, Luis A.

doi:10.1099/mic.0.2008/019471-0

Cited by 260 publications

(294 citation statements)

References 47 publications

Supporting

Mentioning

279

Contrasting

Order By: Relevance

“…Inactivation of the bfmR response regulator, but not the bfmS sensor kinase, resulted in complete abolition of csu expression, resulting in no pili production, loss of adhesive properties, and loss of biofilm formation on plastic (19). In contrast, inactivation of bfmS interfered only partially with biofilm formation and had no influence on downstream target genes (19). As seen with other two-component regulatory systems, this observation suggests that BfmR can use other sensor kinases for its activation.…”

Section: Biofilm Formation and Use Of In Vitro Abiotic Models To Studmentioning

confidence: 67%

“…Despite the importance of the csu operon in biofilm formation on abiotic surfaces, it is less important for adherence to mammalian cells (28). Subsequent work identified a two-component regulatory system for the csu operon, known as bfmRS (19). Inactivation of the bfmR response regulator, but not the bfmS sensor kinase, resulted in complete abolition of csu expression, resulting in no pili production, loss of adhesive properties, and loss of biofilm formation on plastic (19).…”

Section: Biofilm Formation and Use Of In Vitro Abiotic Models To Studmentioning

confidence: 99%

“…Subsequent work identified a two-component regulatory system for the csu operon, known as bfmRS (19). Inactivation of the bfmR response regulator, but not the bfmS sensor kinase, resulted in complete abolition of csu expression, resulting in no pili production, loss of adhesive properties, and loss of biofilm formation on plastic (19). In contrast, inactivation of bfmS interfered only partially with biofilm formation and had no influence on downstream target genes (19).…”

Section: Biofilm Formation and Use Of In Vitro Abiotic Models To Studmentioning

confidence: 99%

See 2 more Smart Citations

Insights into Acinetobacter baumannii pathogenicity

2011

View full text Add to dashboard Cite

SummaryAcinetobacter spp. have justifiably received significant attention from the public, scientific, and medical communities. Over recent years, Acinetobacter, particularly Acinetobacter baumannii, has become a ''red-alert'' human pathogen, primarily because of its exceptional ability to develop resistance to all currently available antibiotics. This characteristic is compounded by its unique abilities to survive in a diverse range of environments, including those within healthcare institutions, leading to problematic outbreaks. Historically, the virulence of the organism has been questioned, but recent clinical reports suggest that Acinetobacter can cause serious, life-threatening infections. Furthermore, its metabolic adaptability gives it a selective advantage in harsh hospital environments. This review focuses on current understanding of A. baumannii pathogenesis and the model systems used to study this interesting organism.

show abstract

Section: Biofilm Formation and Use Of In Vitro Abiotic Models To Studmentioning

confidence: 67%

Section: Biofilm Formation and Use Of In Vitro Abiotic Models To Studmentioning

confidence: 99%

Section: Biofilm Formation and Use Of In Vitro Abiotic Models To Studmentioning

confidence: 99%

See 1 more Smart Citation

Insights into Acinetobacter baumannii pathogenicity

2011

View full text Add to dashboard Cite

show abstract

“…The CsuA/BABCDE usher-chaperone pili assembly system has been involved in A. baumannii biofilm organization on abiotic surfaces [38]. The expression level of csu operon is regulated by the two-component system -BfmS/BfmR, comprising a sensor kinase and a response regulator [39]. Biofilm-associated protein (Bap), expressed on the surface of the bacterial cells, is implicated in cell-to-cell adhesion providing biofilm development and maturation on different substrata [40].…”

Section: Virulence Factorsmentioning

confidence: 99%

Acinetobacter baumannii: biology and drug resistance — role of carbapenemases

Nowak

Paluchowska

2015

Folia Histochem Cytobiol.

107

View full text Add to dashboard Cite

Acinetobacter baumannii is a Gram-negative, glucose-non-fermenting, oxidase-negative coccobacillus, most commonly associated with the hospital settings. The ability to survive in adverse environmental conditions as well as high level of natural and acquired antimicrobial resistance make A. baumannii one of the most important nosocomial pathogens. While carbapenems have long been considered as antimicrobials of last-resort, the rates of clinical A. baumannii strains resistant to these antibiotics are increasing worldwide. Carbapenem resistance among A. baumannii is conferred by coexisting mechanisms including: decrease in permeability of the outer membrane, efflux pumps, production of beta-lactamases, and modification of penicillin-binding proteins. The most prevalent mechanism of carbapenem resistance among A. baumannii is associated with carbapenem-hydrolysing enzymes that belong to Ambler class D and B beta-lactamases. In addition, there have also been reports of resistance mediated by selected Ambler class A carbapenemases among A. baumannii strains. Resistance determinants in A. baumannii are located on chromosome and plasmids, while acquisition of new mechanisms can be mediated by insertion sequences, integrons, transposons, and plasmids. Clinical relevance of carbapenem resistance among strains isolated from infected patients, carriers and hospital environment underlines the need for carbapenemase screening. Currently available methods vary in principle, accuracy and efficiency. The techniques that deserve particular attention belong to both easily accessible unsophisticated methods as well as advanced techniques based on mass spectrometry or molecular biology. While carbapenemases limit the therapeutic options in A. baumannii infections, studies concerning novel beta-lactamase inhibitors offer a new insight into effective therapy.

show abstract

“…In A. baumannii, genomic analyses have identified 12 response regulator genes in an avirulent isolate that increases to 16 to 19 in pathogenic isolates 23 . Of these, only a handful have been experimentally examined [24][25][26][27][28][29] .…”

mentioning

confidence: 99%

A key regulatory mechanism of antimicrobial resistance in pathogenic Acinetobacter baumannii

Adams¹

2017

Microbiol. Aust.

View full text Add to dashboard Cite

Acinetobacter baumannii is a Gram-negative bacterial pathogen that has become a pressing global health issue in recent decades. Although virulence factors for this pathogen have been identified, details of how they are regulated are largely unknown. One widely employed regulatory mechanism that bacteria, such as A. baumannii, have adopted is through two component signal transduction systems (TCS). TCS consist of two proteins; a histidine kinase and response regulator. The histidine kinase allows the bacterium to sense alterations in the extracellular milieu, transmitting the information to the response regulator which prompts the cell to modify gene expression levels accordingly. Bacteria can encode multiple TCS, where each system can mediate specific responses to particular conditions or stressors. Identifying those conditions in which these TCS are expressed, and the genes they regulate known as their ‘regulon', is vital for understanding how A. baumannii survives and persists within the hospital environment or the human host during infection. As we enter the post-antibiotic era, knowledge of TCS could prove to be invaluable, as they offer an alternative target for the treatment of multidrug resistant bacterial infections.

show abstract

Characterization of a two-component regulatory system from Acinetobacter baumannii that controls biofilm formation and cellular morphology

Cited by 260 publications

References 47 publications

Insights into Acinetobacter baumannii pathogenicity

Insights into Acinetobacter baumannii pathogenicity

Acinetobacter baumannii: biology and drug resistance — role of carbapenemases

A key regulatory mechanism of antimicrobial resistance in pathogenic Acinetobacter baumannii

Contact Info

Product

Resources

About