Characterization of a Vibrio-infecting bacteriophage, VPMCC5, and proposal of its incorporation as a new genus in the Zobellviridae family

Kar, Priyanka; Das, Tridip Kr.; Ghosh, Smita; Pradhan, Shrabani; Chakrabarti, Sudipta; Mondal, Keshab Ch; Ghosh, Kuntal

doi:10.1016/j.virusres.2022.198904

Cited by 6 publications

(1 citation statement)

References 36 publications

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“…Additionally, R21Y maintained high lytic activity over varying environmental conditions (4-55 • C and pH 4-10). R21Y showed better thermal tolerance than vB_VhaM_pir03 (45 • C) [21], VPMCC5 (45 • C) [50], vB_VhaS_MAG7 (45 • C) [54], V-YDF132 (50 • C) [49], and vB_VhaS-R18L (50 • C) [53] and greater acid tolerance than V-YDF132 (pH 5-11) and vB_VhaS-R18L (pH 6-11). Thus, R21Y can remain stable and exert bactericidal activity in practical applications, suggesting that it is a promising candidate for biological control of V. harveyi disease.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Vibriophage Infecting Pathogenic Vibrio harveyi

Li,

Yun,

Chen

et al. 2023

IJMS

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Bacterial diseases caused by Vibrio spp. are prevalent in aquaculture and can lead to high mortality rates among aquatic species and significant economic losses. With the increasing emergence of multidrug-resistant Vibrio strains, phage therapy is being explored as a potential alternative to antibiotics for biocontrol of infectious diseases. Here, a new lytic phage named vB_VhaS_R21Y (R21Y) was isolated against Vibrio harveyi BVH1 obtained from seawater from a scallop-farming area in Rongcheng, China. Its morphology, infection cycle, lytic profile, phage stability, and genetic features were characterized. Transmission electronic microscopy indicated that R21Y is siphovirus-like, comprising an icosahedral head (diameter 73.31 ± 2.09 nm) and long noncontractile tail (205.55 ± 0.75 nm). In a one-step growth experiment, R21Y had a 40-min latent period and a burst size of 35 phage particles per infected cell. R21Y was highly species-specific in the host range test and was relatively stable at pH 4–10 and 4–55 °C. Genomic analysis showed that R21Y is a double-stranded DNA virus with a genome size of 82,795 bp and GC content of 47.48%. Its high tolerance and lytic activity indicated that R21Y may be a candidate for phage therapy in controlling vibriosis in aquacultural systems.

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