Characterization of a wheat transcription factor, TaWRKY45, and its effect on Fusarium head blight resistance in transgenic wheat plants

Bahrini, Insaf; Sugisawa, Motoki; Kikuchi, Rie; Ogawa, Taiichi; Kawahigashi, Hiroyuki; Ban, Tomohiro; Handa, Hirokazu

doi:10.1270/jsbbs.61.121

Cited by 34 publications

(28 citation statements)

References 42 publications

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“…In the present study, LR treatment induced the expression of four WRKY transcription factors in KU-2084, namely WRKY24 (homolg of TaWRKY41), WRKY62 (homlog of Tawrky15), WRKY70 (homolog of TaWRKY 45), and WRKY72 (homolog of TaWRKY19). As TaWRKY45 reportedly enhances transgenic wheat resistance to Fusarium head bright [23] and several WRKY members are involved in PAMP recognition of pathogen threat in plants [8,37], the upregulation of these WRKY genes in infected KU-2084 may represent a mechanism of either plant-pathogenic response or pathogenic defense.…”

Section: Discussionmentioning

confidence: 99%

Characterization of two leaf rust-resistant Aegilops tauschii accessions for the synthetic wheat development

et al. 2020

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Aegilops tauschii (Ae. tauschii) is a diploid (2n = 2x = 14) wild grass species, which has been reported as the progenitor of hexaploid wheat (Triticum aestivum) with D-genome. In this study, 68 Ae. tauschii accessions with diverse geographical backgrounds were investigated for their resistance to infection by the leaf rust fungi Puccinia triticina. Two Ae. tauschii accessions that exhibited hyper-resistance to leaf rust at both seedling and adult stages were identified. Utilizing two susceptible Ae. tauschii ecotypes and keumkang, a common Korean wheat cultivar known to be susceptible to leaf rust, as the negative control, further investigations were conducted for understanding the mechanism underlying immunity to leaf rust disease of these two resistant accessions. Resistant accessions displayed the increased β-1,3-glucanase activity to prevent fungal penetration and the better peroxidase activity to cope with leaf rustinduced oxidative stress. Moreover, transcriptional analyses reveal the important role of the LRR receptor-like serine/ threonine-protein kinase FLS2 (lrr) to the disease resistance of the two ecotypes. Ae. tauschii is a remarkable genetic source, especially for abiotic and biotic stress resistance genes, as the plant is known for its wide-ranging geographical habitat and adaptability to different environments. This, combined with the fact that Ae. tauschii and wheat share a close evolutionary relationship, is indicative of the immense benefit of using Ae. tauschii as a material for improving the quality of synthetic wheat. Our aim was to identify and evaluate the strongest Ae. tauschii contenders for breeding leaf rust-resistant synthetic wheat.

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Section: Discussionmentioning

confidence: 99%

Characterization of two leaf rust-resistant Aegilops tauschii accessions for the synthetic wheat development

et al. 2020

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“…More and more results demonstrated that over-expression of stress responsive transcription factors is a promising strategy for improving abiotic stress tolerance in crop [26,27]. Some members of WRKY family have been found to be responsive to various abiotic stresses [28].…”

Section: Discussionmentioning

confidence: 99%

A wheat salinity-induced WRKY transcription factor TaWRKY93 confers multiple abiotic stress tolerance in Arabidopsis thaliana

Qin

Tian

Liu

2015

Biochemical and Biophysical Research Communications

129

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“…The WRKY transcription factors have been known in the regulation of transcriptional reprogramming associated with plant immune responses [ 70 , 71 ]. The constitutive overexpression of WRKY conferred an enhanced resistance against F. graminearum in transgenic wheat plants grown under greenhouse conditions [ 72 ]. The TaWRKY70 from wheat acts as a positive regulator of resistance against F. graminearum [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

A Comparative Transcriptome Analysis, Conserved Regulatory Elements and Associated Transcription Factors Related to Accumulation of Fusariotoxins in Grain of Rye (Secale cereale L.) Hybrids

Mahmood

Orabi²,

Kristensen³

et al. 2020

IJMS

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Detoxification of fusariotoxin is a type V Fusarium head blight (FHB) resistance and is considered a component of type II resistance, which is related to the spread of infection within spikes. Understanding this type of resistance is vital for FHB resistance, but to date, nothing is known about candidate genes that confer this resistance in rye due to scarce genomic resources. In this study, we generated a transcriptomic resource. The molecular response was mined through a comprehensive transcriptomic analysis of two rye hybrids differing in the build-up of fusariotoxin contents in grain upon pathogen infection. Gene mining identified candidate genes and pathways contributing to the detoxification of fusariotoxins in rye. Moreover, we found cis regulatory elements in the promoters of identified genes and linked them to transcription factors. In the fusariotoxin analysis, we found that grain from the Nordic seed rye hybrid “Helltop” accumulated 4 times higher concentrations of deoxynivalenol (DON), 9 times higher nivalenol (NIV), and 28 times higher of zearalenone (ZEN) than that of the hybrid “DH372” after artificial inoculation under field conditions. In the transcriptome analysis, we identified 6675 and 5151 differentially expressed genes (DEGs) in DH372 and Helltop, respectively, compared to non-inoculated control plants. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEGs were associated with glycolysis and the mechanistic target of rapamycin (mTOR) signaling pathway in Helltop, whereas carbon fixation in photosynthesis organisms were represented in DH372. The gene ontology (GO) enrichment and gene set enrichment analysis (GSEA) of DEGs lead to identification of the metabolic and biosynthetic processes of peptides and amides in DH372, whereas photosynthesis, negative regulation of catalytic activity, and protein-chromophore linkage were the significant pathways in Helltop. In the process of gene mining, we found four genes that were known to be involved in FHB resistance in wheat and that were differentially expressed after infection only in DH372 but not in Helltop. Based on our results, we assume that DH372 employed a specific response to pathogen infection that led to detoxification of fusariotoxin and prevented their accumulation in grain. Our results indicate that DH372 might resist the accumulation of fusariotoxin through activation of the glycolysis and drug metabolism via cytochrome P450. The identified genes in DH372 might be regulated by the WRKY family transcription factors as associated cis regulatory elements found in the in silico analysis. The results of this study will help rye breeders to develop strategies against type V FHB.

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Characterization of a wheat transcription factor, TaWRKY45, and its effect on Fusarium head blight resistance in transgenic wheat plants

Cited by 34 publications

References 42 publications

Characterization of two leaf rust-resistant Aegilops tauschii accessions for the synthetic wheat development

Characterization of two leaf rust-resistant Aegilops tauschii accessions for the synthetic wheat development

A wheat salinity-induced WRKY transcription factor TaWRKY93 confers multiple abiotic stress tolerance in Arabidopsis thaliana

A Comparative Transcriptome Analysis, Conserved Regulatory Elements and Associated Transcription Factors Related to Accumulation of Fusariotoxins in Grain of Rye (Secale cereale L.) Hybrids

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