Characterization of a world collection of Agropyron cristatum accessions

Copete, Alejandro; Moreno, Roberto; Cabrera, Adoración

doi:10.1007/s10722-018-0630-9

Cited by 10 publications

(11 citation statements)

References 42 publications

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“…We detected Bs only in crested wheatgrass accession PI 499388, and this observation is consistent with Copete et al (2018) who studied 115 crested wheatgrass accessions and reported the existence of Bs only in the diploid accession PI 499388. However, in their work, they did not mention the Bs copy number per cell.…”

Section: Discussionsupporting

confidence: 92%

Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting

et al. 2019

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Core Ideas Flow sorting enabled to dissect the genome of crested wheatgrass into chromosomes. This is a powerful approach to access the complex genome of a wheat wild relative. Chromosome genomics will support alien introgression breeding of the crop. Wheatgrass (Agropyron sp.) is a potential source of beneficial traits for wheat improvement. Among them, crested wheatgrass [A. cristatum (L.) Gaertn.] comprises a complex of diploid, tetraploid, and hexaploid forms with the basic genome P, with some accessions carrying supernumerary B chromosomes (Bs). In this work, we applied flow cytometry to dissect the complex genome of crested wheatgrass into individual chromosomes to facilitate its analysis. Flow karyotypes obtained after the analysis of 4ʹ,6‐diamidino‐2‐phenylindole (DAPI)‐stained mitotic chromosomes of diploid and tetraploid accessions consisted of three peaks, each corresponding to a group of two or three chromosomes. To improve the resolution, bivariate flow karyotyping after fluorescent labeling of chromosomes with fluorescein isothiocyanate (FITC)‐conjugated probe (GAA)7 microsatellite was applied and allowed discrimination and sorting of P genome chromosomes from wheat–crested wheatgrass addition lines. Chromosomes 1P–6P and seven telomeric chromosomes could be sorted at purities ranging from 81.7 to 98.2% in disomics and from 44.8 to 87.3% in telosomics. Chromosome 7P was sorted at purities reaching 50.0 and 39.5% in diploid and tetraploid crested wheatgrass, respectively. In addition to the whole complement chromosomes (A), Bs could be easily discriminated and sorted from a diploid accession at 95.4% purity. The sorted chromosomes will streamline genome analysis of crested wheatgrass, facilitating gene cloning and development of molecular tools to support alien introgression into wheat.

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Section: Discussionsupporting

confidence: 92%

Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting

et al. 2019

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“…The diploids are less common and distributed from Europe to Mongolia, whereas tetraploids are widespread, in central Europe, the Middle East and central Asia. Hexaploids are rare and are mainly found in Turkey, Iran and Georgia (Dewey and Asay 1982; Copete et al 2018). The nature of the polyploid genome of A. cristatum and the origin of its P genome(s) have not yet been clarified.…”

Section: Introductionmentioning

confidence: 99%

Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers

et al. 2019

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Key messageUsing COS markers, the study reveals homeologous relationships between tetraploid Agropyron cristatum and bread wheat to support alien introgression breeding of wheat.AbstractCrested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat that possesses many genes that are potentially useful in wheat improvement. The species comprises a complex of diploid, tetraploid and hexaploid forms. In this study, wheat–A. cristatum chromosome, telosome and translocation lines were used to characterize syntenic relationships between tetraploid A. cristatum and bread wheat. Prior to mapping COS markers, the cytogenetic stock lines were characterized for fertility and by FISH and GISH for karyotype stability. Out of 328 COS markers selected for the study, 279 consistently amplified products in tetraploid A. cristatum, and, out of these, 139 were polymorphic between tetraploid crested wheatgrass and wheat. Sixty-nine markers were found to be suitable for the detection of tetraploid A. cristatum chromosomes 1P–6P in wheat, ranging from 6 to 17 markers per chromosome. BLASTn of the source ESTs resulted in significant hits for 67 markers on the wheat pseudomolecules. Generally, COS markers of the same homeologous group were detected on similar arms in both Agropyron and wheat. However, some intragenomic duplications and chromosome rearrangements were detected in tetraploid A. cristatum. These results provide new insights into the structure and evolution of the tetraploid A. cristatum genome and will facilitate the exploitation of the wild species for introgression breeding of bread wheat.Electronic supplementary materialThe online version of this article (10.1007/s00122-019-03394-1) contains supplementary material, which is available to authorized users.

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“…The P genome from A. cristatum contains a high genetic diversity, which has been demonstrated by cytological, molecular, and morphological data [33,34]. The A. cristatum genome has also been considered a valuable source of genes for pest resistance and abiotic stresses in the framework of wide hybridization programs to improve cereal crops [2,35].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Chromosome Associations during Early Meiosis in Wheat Lines Carrying Chromosome Introgressions from Agropyron cristatum

Prieto

Palomino

Cifuentes

et al. 2021

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Crested wheatgrass (Agropyron cristatum L. Gaertn., genome P), included in the Triticeae tribe (family Poaceae), is one of the most important grasses in temperate regions. It has been valued as a donor of important agronomic traits for wheat improvement, including tolerance to cold, drought, and high salinity, as well as resistance to leaf rust, stripe rust, and powdery mildew. For successful incorporation of beneficial alleles into wheat, it is essential that recombination between wheat and A. cristatum chromosomes occurs. In this work, we analysed chromosome associations during meiosis in wheat lines carrying chromosome introgressions from A. cristatum chromosomes 5P and 6P in the presence and absence of Ph1 locus using fluorescence in situ hybridisation. The results showed that the Ph1 locus does not affect chromosome associations between A. cristatum and wheat chromosomes because there were no interspecific chromosome associations; therefore, no recombination between chromosomes from wheat and Agropyron were observed in the absence of the Ph1 locus. The 5P and 6P A. cristatum chromosomes do not have a suppressor effect on the Ph1 locus. Wheat univalents in metaphase I suggest that Agropyron chromosomes might carry genes having a role in wheat homologous chromosome associations. Putative effect of the Agropyron genes on wheat chromosome associations does not interact with the Ph1 locus.

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Characterization of a world collection of Agropyron cristatum accessions

Cited by 10 publications

References 42 publications

Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting

Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting

Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers

Analysis of Chromosome Associations during Early Meiosis in Wheat Lines Carrying Chromosome Introgressions from Agropyron cristatum

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