Characterization of a xenograft model for anti-CD19 CAR T cell studies

Ahmadbeigi, Naser; Alatab, Sudabeh; Vasei, Mohammad; Ranjbar, Azam; Aghayan, Seyed Sajjad; Khorsand, Amir Arsalan; Moradzadeh, Kobra; Darvishyan, Z; Jamali, M; Muhammadnejad, Samad

doi:10.1007/s12094-021-02626-5

Cited by 4 publications

(1 citation statement)

References 30 publications

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“…The presence of CD3 + lymphocytes in the blood compartment of the treated mice was investigated by flow cytometry, as previously described, 26 to evaluate the engraftment possibility of intrusive T cells.…”

Section: Methodsmentioning

confidence: 99%

Adoptive NK‐cell transfer as a potential treatment paradigm for Wilms tumor: A preclinical study

Behfar

Muhammadnejad

Abdolahi

et al. 2022

Pediatric Blood & Cancer

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Background Natural killer (NK) cell therapy has been shown to be effective in the treatment of some cancers. However, the effects of this adoptive immunotherapy have not been investigated for Wilms tumor (WT). In this study, the effects of adoptive NK‐cell transfer on a patient‐derived xenograft (PDX) model of anaplastic WT were evaluated, and the impacts of cell source and ex vivo activation strategy on the therapeutic efficacy of NK‐cell product were appraised. Methods NK cells were isolated from human peripheral blood mononuclear cells (NKPB) and human cord blood (NKCB), and were expanded and activated using a cytokine cocktail. Another group of NK cells (NKET) was produced through activation with the exosomes extracted from previously challenged NKPB cells with WT. PDX‐bearing mice were treated with clinically relevant doses of NKPB, NKCB, NKET, standard chemotherapy, and placebo (phosphate‐buffered saline). Results PDX models treated with NKCB showed a better survival rate, though the difference among the study groups was not significant. Compared with the placebo control group, NKCB significantly improved the histopathologic response, NKPB significantly inhibited the proliferation of neoplastic cells, and NKET led to a significant decrease in the metastasis score (all p‐values <.05). Standard chemotherapy provided the greatest tumor growth inhibition and the lowest mitotic count, though it did not show any significant advantage over NK‐cell therapies in any of the outcome parameters in two‐by‐two comparisons. Conclusions This study spotlights the efficacy of adoptive NK‐cell transfer as a potential treatment candidate for high‐risk WT.

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Section: Methodsmentioning

confidence: 99%