Characterization of Acetamiprid Biodegradation by the Microbial Consortium ACE-3 Enriched From Contaminated Soil

Abstract

Microbial consortia are ubiquitous in nature and exhibit several attractive features such as sophisticated metabolic capabilities and strong environmental robustness. This study aimed to decipher the metabolic and ecological characteristics of synergistic interactions in acetamiprid-degrading consortia, suggesting an optimal scheme for bioremediation of organic pollutants. The microbial consortium ACE-3 with excellent acetamiprid-degrading ability was enriched from the soil of an acetamiprid-contaminated site and characterized using high-throughput sequencing (HTS). Consortium ACE-3 was able to completely degrade 50 mg•L −1 acetamiprid in 144 h, and was metabolically active at a wide range of pH values (6.0-8.0) and temperatures (20-42 • C). Furthermore, plausible metabolic routes of acetamiprid biodegradation by the consortium were proposed based on the identification of intermediate metabolites (Compounds I, II, III and IV). The findings indicated that the consortium ACE-3 has promising potential for the removal and detoxification of pesticides because it produces downstream metabolites (Compounds I and II) that are less toxic to mammals and insects than acetamiprid. Finally, Illumina HTS revealed that β Proteobacteria were the dominant group, accounting for 85.61% of all sequences at the class level. Among the more than 50 genera identified in consortium ACE-3, Sphingobium, Acinetobacter, Afipia, Stenotrophomonas, and Microbacterium were dominant, respectively accounting for 3.07, 10.01, 24.45, and 49.12% of the total population.

Efficacy of novel bacterial consortia in degrading fipronil and thiobencarb in paddy soil: a survey for community structure and metabolic pathways

“…Subsequently, the consortia were resuspended in sterilized MSM, supplemented with 40% glycerin, resulting in a final cell concentration of approximately 10 8 cells/ml. The inocula were then cryopreserved at −80°C for subsequent experiments and long-term usage ( Xu et al, 2020 ; Pang et al, 2023 ).…”

Microbial Technologies Employed for Biodegradation of Neonicotinoids in the Agroecosystem

“…After 48 h of incubation, at 200 mg/L, about 70% degradation of acetamiprid was achieved. The degradation of thiamethoxam was investigated in municipal wastewater, and the microbial action accelerated dissipation by UV light to degrade acetamiprid in the contaminated environment, a bacterial strain ACE-3 was isolated from soil, and results indicated that this strain was able to degrade acetamiprid entirely at the initial concentration of 50 mg/L within 144 hours in a broad range of environmental conditions such as pH (6–8) and temperature (20–42 °C) ( Peña et al, 2011 ; Xu et al, 2020 ).…”

Cutting Edge Use of Microbial Consortia for Bioremediation of Contaminated Soil

“…The existence and activity of an appropriate microbial consortia is one of the critical components that will affects bioremediation success (Kebede et al, 2021). A microbial consortium is any two or more microbial communities of distinct species or genera acting synergistically in a complicated system (Xu et al, 2020) which can be natural or artificial. For enhanced bioremediation of soil contaminants, a microbial consortium can be more advantageous than a single strain in a number of ways.…”

“…The development and use of microbial consortia for the bioremediation of soil contaminants still has numerous obstacles and knowledge gaps. According to Xu et al (2020) the development of effective and stable microbial consortia from natural or contaminated soils involves characterizing and optimizing their metabolic pathways and interactions involved in pollutant degradation, evaluating and enhancing the bioavailability and biodegradability of soil pollutants by microbial consortia, and assessing and monitoring the ecological effects and risks of introducing microbes.…”