Characterization of acid-soluble collagen from the skin of walleye pollock (Theragra chalcogramma)

Yan, Mingyan; Li, Bafang; Zhao, Xue; Ren, Guoyan; Zhuang, Yongliang; Hou, Hu; Xiu-kun, Zhang; Chen, Li; Fan, Yan

doi:10.1016/j.foodchem.2007.10.027

Cited by 131 publications

(94 citation statements)

References 28 publications

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“…Previous works carried out with different species such as fish showed the same behaviour; however, the range of temperature was observed between 25°C and 32°C. (Huang et al, 2011;Nalinanon et al, 2007;Safandowska & Pietrucha, 2013;Yan et al, 2008;Zhang et al, 2007). The thermal stability of collagen obeys to the matrix of hydrogen bonds.…”

Section: Viscositymentioning

confidence: 99%

“…The denaturation temperature of collagen is an irreversible process where the triple helix structure is lost due to the rupture of hydrogen bonds (Rochdi, Foucat, & Renou, 2000). The typical denaturation temperature of collagen is around 40°C; however, this temperature value varies from species to species, and the denaturation temperature of fish collagen is lower than that of collagen extracted from mammals (Huang et al, 2011;Yan et al, 2008;Zhang et al, 2007).…”

Section: Thermal Propertiesmentioning

confidence: 99%

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Extraction and characterization of collagen from rabbit skin: partial characterization

Martínez-Ortiz

Hernández-Fuentes

Pimentel-González

et al. 2014

CyTA - Journal of Food

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(2015) Extraction and characterization of collagen from rabbit skin: partial characterization, CyTAJournal of Food, 13:2, 253-258, DOI: 10.1080/19476337.2014 To link to this article: https://doi.org/10. 1080/19476337.2014 Extraction and characterization of collagen were carried out in rabbit skins as a new alternative for collagen type I. Acetic acid and pepsin were for the extraction of soluble and insoluble collagen, respectively. The enzymatic treatment yielded higher amount of collagen (71%). The average pH value was 6.3, no matter what the method of extraction was. Denaturation temperature of collagen was found at 36°C approximately in two different techniques: Rheometer and Differential Scanning Calorimetry (DSC). Despite the amount of collagen in solution was low, its viscosity was high because of the hydrodynamic behaviour of collagen molecules. Sodium dodecyl sulphate polyacrylamide gel electrophoresis results showed three different bands that reflected two alpha-chains and one beta-chain with molecular weights of 102, 118 and 220 kDa, respectively. Determination of hydroxyproline gave evidence that the extracted material was collagen. It was concluded that rabbit skin could be an alternative source for the extraction of collagen.Keywords: collagen; rabbit skin; extraction; thermal analysis Se realizó la extracción y caracterización de colágeno en pieles de conejo como una nueva alternativa para colágeno tipo I. Se usaron ácido acético y pepsina para la extracción de colágeno soluble e insoluble respectivamente. El tratamiento enzimático fue el que rindió alta cantidad de colágeno (71%). El pH promedio fue de 6,3 sin importar cuál fue el método de extracción. La temperatura de desnaturalización del colágeno se encontró a 36°C aproximadamente mediante dos diferentes técnicas: Reometría y DSC. A pesar de que la cantidad de colágeno en la solución fue bajo, su viscosidad fue alta debido a el comportamiento hidrodinámico de las moléculas. Los resultados de SDS-PAGE mostraron tres diferentes bandas en las que se reflejaron dos cadenas alfa y una cadena beta con pesos moleculares de 102, 118 and 220 kDa respectivamente. La determinación del contenido de hidroxiprolina dio evidencia de que el material extraído fue colágeno. Se concluyó que la piel de conejo podría ser una alternativa de materia prima para la extracción de colágeno.

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Section: Viscositymentioning

confidence: 99%

Section: Thermal Propertiesmentioning

confidence: 99%

Extraction and characterization of collagen from rabbit skin: partial characterization

Martínez-Ortiz

Hernández-Fuentes

Pimentel-González

et al. 2014

CyTA - Journal of Food

View full text Add to dashboard Cite

show abstract

“…Differential scanning calorimetry (DSC) was performed on a Netzsch DSC 200PC (Netzsch, Bavaria, Germany) fitted with an air cooling compressor and a liquid nitrogen cooler at ambient temperature (Yan et al, 2008). The temperature was calibrated effectively using indium as standard.…”

Section: Differential Scanning Calorimetrymentioning

confidence: 99%

“…The skin is dumped without utilization except for use in food feed, causing environmental pollution and resources waste. However, 70% of the pollock skin dry matter is collagen (Yan et al, 2008;Zhang, Luo, Zhang, Song, & Jiang, 2003). For making effective use of the dumped skin as a gelatin resource, it is necessary to study the characteristics of the pollock skin gelatin.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical properties of gelatin gels from walleye pollock (Theragra chalcogramma) skin cross-linked by gallic acid and rutin

Yan

Zhao

et al. 2011

Food Hydrocolloids

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“…Collagen was prepared according to the method of Nagai and Suzuki (2000) with a slight modification. The extraction procedure and characterisation of collagen were shown in the paper of Yan et al (2008).…”

Section: Preparation Of Collagenmentioning

confidence: 99%

Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene

Yan

Zhao

2010

Food Chemistry

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Characterization of acid-soluble collagen from the skin of walleye pollock (Theragra chalcogramma)

Cited by 131 publications

References 28 publications

Extraction and characterization of collagen from rabbit skin: partial characterization

Extraction and characterization of collagen from rabbit skin: partial characterization

Physicochemical properties of gelatin gels from walleye pollock (Theragra chalcogramma) skin cross-linked by gallic acid and rutin

Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene

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