Characterization of acid soluble collagen (ASC) and pepsin soluble collagen (PSC) extracted from shortfin scad (Decapterus macrosoma) waste

Abstract

Fish waste management is one of the biggest impacts towards the environment. However, it is one of the rich sources of protein. Therefore, the aim of this study was to isolate and characterize acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from shortfin scad (Decapterus macrosoma) waste, with the yields of ASC (3.35±3.43%) and PSC (0.10±0.13%) respectively. The isolated ASC and PSC from shortfin scad waste were characterized on chemical composition, protein concentration, structural properties by FTIR, solubility and morphology properties compared to the commercial collagen from tilapia (Oreochromis niloticus) scales. In terms of chemical composition, PSC showed better than ASC, since it contains comparatively higher in protein (26.97±1.02%) by Kjeldahl method and protein concentration (2.69±0.47 mg/mL) by biuret method, compared to ASC. The FTIR investigations also certified the existence of spacial triplehelical structures of collagens that are important for collagen backbones. Solubility results showed that both ASC and PSC are high insolubility at acidic pH with the lowest solubilisation point at the range of alkali condition. The morphological analysis of both ASC and PSC were almost similar too, as there were fibrils and porous structure. Therefore, collagen from shortfin scad waste, could be a useful alternative, with the potential to be used in food ingredient, biomedical, pharmaceutical and nutraceuticals industries

In Vitro Antioxidant and Antiaging Activities of Collagen and Its Hydrolysate from Mackerel Scad Skin (Decapterus macarellus)

“…This means that every 100 g of skin (wet weight) could produce 6.39 g of collagen. The resulting yield was 60 times greater than the collagen yield of Decapterus macrosoma species, which was found to be only 0.10 ± 0.13% ( w / w ) [ 18 ]. The main differences with respect to the extraction method in the two Decapterus genera above are the pepsin concentration and the duration of immersion.…”

“…The high solubility of HC was most likely because of the smaller molecular size of the produced peptides, as well as the release of polar carboxyl and amide groups, which have a high water interaction capacity during the hydrolysis of peptide bonds in the collagen structure. Generally, PSC has a solubility range of 20–100% [ 18 ]. PSC can also be more soluble in acidic pH (1–4) and less soluble in iso-electric point (pI) [ 28 ].…”

“…In the current study, collagen was successfully extracted using the acid–pepsin method. During immersion, acetic acid further swells the fish skin, interferes with the hydrophobic and electrostatic interactions between the tropocollagen units, and releases collagen [ 18 ]. Meanwhile, pepsin acts on the nonhelical peptide chain of collagen and has no effect on the helical peptide chain of collagen (tropocollagen).…”

Effect of Extraction Time on the Extractability and Physicochemical Properties of Pepsin—Soluble Collagen (PCS) from the Skin of Silver Catfish (Pangasius sp.)

“…Collagen is a vital animal protein, constituting around 30% of total protein, making it the most abundant fibrous protein in animals [ 1 , 2 ]. It is essential in forming connective tissues such as bones, tendons, skin, and the vascular system [ 3 , 4 ].…”

Physicochemical and thermal properties of pepsin‐ and acid‐soluble collagen isolated from the body wall of sea cucumbers (Stichopus hermanni)

“…The most prevalent protein in mammals and a fibrous protein in all multicellular organisms is collagen. It functions as a supporting component in the extracellular matrix (Shaik et al, 2023;Sulaiman & Sarbon, 2020). The skin, tendons, bones, cartilage, and tissue are where collagen is primarily found.…”