Characterization of Acinetobacter baumannii strain PS3 in degradation of food emulsifiers

Nguyễn, Ngọc Tuấn; Tran, Tuyet Nhung; Ha, Thi Bich Ngoc

doi:10.1063/1.5033393

Cited by 2 publications

(7 citation statements)

References 15 publications

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“…However, in the present research, it has been demonstrated that Tween 80 can be used as sole source of carbon and energy by F. culmorum. Nguyen et al (2018) reported that Acinetobacter baumannii was capable of utilizing Tween 80 as its sole carbon and energy source. It was suggested that the mechanism for polysorbate utilization is via β-oxidation, which would involve cleavage of oleate ester linkage by an esterase.…”

Section: Discussionmentioning

confidence: 99%

“…The cleavage of the ether linkages would form primary alcohol. Alcohols would be subsequently oxidized to aldehydes and then to carboxylic acids which would enter the Krebs cycle to be mineralized to CO2 and H2O (Nguyen et al, 2018). Li et al (2018) reported that Tween 80 increased the biomass and the intracellular polysaccharide content in Lentinula edodes grown under liquid fermentation conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Due to the widespread use of Tween 80, it is important to understand how this surfactant affects microbial growth and enzyme production. Some studies have reported that microorganisms are capable of utilizing Tween 80 as a sole source of carbon (Minami, 1958;Chakrabarty et al, 1970;Nielsen et al, 2016;Nguyen et al, 2018). Fungi are of great importance for bioremediation process due to the highly efficient intracellular and extracellular enzymatic system (Sánchez, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Effect of surfactant Tween 80 on growth and esterase production of Fusarium culmorum in liquid fermentation

Medina-Flores¹,

González-Marquez²,

Sánchez³

2020

Mexjbiotechnol

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Tween 80 is a widely used non-ionic surfactant that is added to culture media to make hydrophobic substrate available to microorganisms. Because of this surfactant widespread use, it is important to understand how it affects microbial growth and enzyme production. In this work, the effect of different concentrations (100, 400 and 600 l/L; v/v) of Tween 80 (as the sole carbon source) on the biomass production, esterase activities (assessed through biochemical tests and zymographic assays) and protein content of Fusarium culmorum grown in liquid fermentation was determined. The specific growth rate (μ), biomass yield (YX/S), esterase yield (YE/X), esterase productivity (P), maximal enzymatic activity (Emax), and specific rate of enzyme production (qp) were also estimated. A control medium added with glucose was used. The highest μ was showed in the medium added with 100 l of Tween 80/L. However, the greatest esterase activity was observed in those media containing the highest concentrations of Tween 80 ( energy source and it also induced the esterase production by F. culmorum. Tween 80 concentration is positively correlated with the number of esterase isoforms produced by this fungus. The higher the Tween 80 concentration (400 μl/L and 600 μl/L, v/v), the more number of esterase isoforms will be induced. However, lower concentration (100 μl/L) of Tween 80/L did not show a significant effect on the induction of the esterase activity.400 l/L and 600 l/L; v/v). These results show that Tween 80 was used as the sole carbon and

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of surfactant Tween 80 on growth and esterase production of Fusarium culmorum in liquid fermentation

Medina-Flores¹,

González-Marquez²,

Sánchez³

2020

Mexjbiotechnol

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“…It has been reported that Tween 80 can be mineralized to CO2 and H2O through β-oxidation. In this context, the cleavage of the ether linkages would form primary alcohol, which would be subsequently oxidized to aldehydes and then to carboxylic acids which would enter the Krebs cycle (Nguyen et al, 2018). Several studies have reported that Tween 80 can be used as carbon and energy source by microorganisms and/or as enzyme inducer.…”

Section: Discussionmentioning

confidence: 99%

“…In this context, it is important to understand how Tween 80 affects microbial growth and enzyme production. Some investigations have described that microorganisms are able to use Tween 80 as a sole source of carbon (Chakrabarty et al, 1970;Nielsen et al, 2016;Nguyen et al, 2018). It has been found that Fusarium culmorum was able to use Tween 80 as the sole carbon and energy source and that esterase production was induced by this surfactant (Medina-Flores et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Tween 80-induced esterase production by Trichoderma harzianum in submerged fermentation: An esterase activity assay using α-naphthyl acetate as substrate

González-Marquez¹,

Sánchez²

2022

Mexjbiotechnol

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Tween 80 is a surfactant that has been used for several purposes in microbial cultures. In bioremediation studies, the addition of surfactants in the medium is a common practice, since it makes hydrophobic substrates available to the microorganism. In this work, the influence of Tween 80 (100 or 400 μL/L) on the biomass production, protein content, esterase activities and, growth and enzymatic yield parameters of Trichoderma harzianum grown in submerged fermentation was evaluated. Assessments of esterase activities through biochemical tests and zymographic assays were carried out using α-naphthyl acetate as substrate. A single band with esterase activity was observed in the different concentrations of Tween 80 tested. However, 400 μL of Tween 80 induced higher esterase production (148.2 U/L) in T. harzianum as compared to that esterase activity (79.5 U/L) shown in the medium added with 100 μL of Tween 80 per liter. These results showed that high concentrations (e.g. 400 μL/L) of Tween 80 enhanced the esterase activity in T. harzianum. The single band with esterase activity revealed on zymograms suggests that this enzyme (of 38 kDa approx.) may be present in both forms as a constitutive as well as an inducible esterase, since it was observed in both glucose- and Tween 80-supplemented media.

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Characterization of Acinetobacter baumannii strain PS3 in degradation of food emulsifiers

Cited by 2 publications

References 15 publications

Effect of surfactant Tween 80 on growth and esterase production of Fusarium culmorum in liquid fermentation

Effect of surfactant Tween 80 on growth and esterase production of Fusarium culmorum in liquid fermentation

Tween 80-induced esterase production by Trichoderma harzianum in submerged fermentation: An esterase activity assay using α-naphthyl acetate as substrate

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