Characterization of activated sludge in wastewater treatment processes using front-face excitation–emission matrix (FF-EEM) fluorescence spectroscopy

Yu, Hui; Li, Yimeng; Yang, Haiyang; Lv, Yong; Wang, Jingyin; Rong, Hongwei; Qu, Fangshu

doi:10.1039/d2ew00315e

Cited by 5 publications

(2 citation statements)

References 41 publications

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“…19,20 It has also been applied to investigate fluorophore in a turbid coagulation suspension or in activated sludge. 21,22 Most freshwater cyanobacteria possess intrinsic fluorescence, i.e. phycocyanin, with an excitation wavelength of 590−630 nm and an emission maximum lies around 650 nm.…”

Section: ■ Introductionmentioning

confidence: 99%

“…However, the traditional EEM could not analyze suspension solutions or solid samples. Recently, front-face EEM (FF-EEM) was proposed to analyze the membrane surface deposited by model foulants or natural organic matters (NOM). , It has also been applied to investigate fluorophore in a turbid coagulation suspension or in activated sludge. , Most freshwater cyanobacteria possess intrinsic fluorescence, i.e. phycocyanin, with an excitation wavelength of 590–630 nm and an emission maximum lies around 650 nm .…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Front-Face Fluorescence for Assessing Cyanobacteria Fouling in Ultrafiltration

Yu,

Huang,

Zhong

et al. 2023

Environ. Sci. Technol.

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Cyanobacteria fouling in ultrafiltration (UF) drinking water treatment poses a significant threat to the stability and sustainability of the process. Both phycocyanin found in cyanobacteria and the polymer membrane exhibit strong fluorescence, which could be readily detected using front-face excitation–emission matrix (FF-EEM) spectroscopy. In this study, FF-EEM was employed for the nondestructive and in situ characterization of algae fouling evolution in UF, while also analyzing fouling mechanisms and reversibility. The results indicated that phycocyanin fluorescence on the membrane surface showed a linear correlation with the specific algal cell count on the membrane surface before reaching saturation. As fouling progressed, membrane fluorescence decreased, which was associated with the extent of the surface coverage on the membrane. The plateau in membrane fluorescence indicated full coverage, coinciding with the cake filtration mechanism, cake compression, and deterioration of fouling reversibility. These findings highlight the promise of FF-EEM as a valuable tool for monitoring and evaluating fouling of cyanobacteria in UF systems.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of Front-Face Fluorescence for Assessing Cyanobacteria Fouling in Ultrafiltration

Yu,

Huang,

Zhong

et al. 2023

Environ. Sci. Technol.

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Modulable 3D-printed plantibody-laden platform enabling microscale affinity extraction and ratiometric front-face fluorescence detection of microcystin-LR in marine waters

Payà-Pou,

Aguirre-Camacho,

Simó-Alfonso

et al. 2024

Microchim Acta

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A 3D-printed stereolithographic platform for selective biorecognition is designed to enable convective microscale affinity extraction of microcystin-LR (MC-LR) followed by direct solid-phase optosensing exploiting ratiometric front-face fluorescence spectroscopy. For this purpose, a recombinant monoclonal plantibody (recAb) is covalently attached to a 3D-printed structure for sorptive immunoextraction, whereupon the free and unbound primary amino moieties of the recAb are derivatized with a fluorescent probe. The fluorophore-recAb-MC-LR laden device is then accommodated in the cuvette holder of a conventional fluorometer without any instrumental modification for the recording of the solid-phase fluorescence emission. Using Rodbard’s four-parameter sigmoidal function, the 3D-printed bioselective platform features a limit of detection (LOD) of 28 ng L−1 using a sample volume of 500 mL, device-to-device reproducibility down to 12%, and relative recoveries ranging from 91 to 100% in marine waters. Printed prototypes are affordable, just 0.4 € per print and ≤ 10 € per device containing recAb. One of the main assets of the miniaturized immunoextraction device is that it performs comparably well in terms of analytical figures of merit with costly mass spectrometric-based analytical methodologies, such as HPLC–MS/MS. The device is readily applicable to high-matrix samples, such as seawater, as opposed to previous biosensing platforms, just applied to freshwater systems. Graphical abstract

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Solid-phase fluorescence: Reproducibility and comparison with the solution states

Nakaya,

Tomita,

Yamamura

2024

Talanta

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Characterization of activated sludge in wastewater treatment processes using front-face excitation–emission matrix (FF-EEM) fluorescence spectroscopy

Abstract: Rapid, reliable assay, and on-line monitoring for activated sludge have always been urgently demanded in wastewater treatment processes. In this work, the front-face fluorescence excitation-emission matrix (FF-EEM) spectroscopy was employed...

Cited by 5 publications

References 41 publications

Evaluation of Front-Face Fluorescence for Assessing Cyanobacteria Fouling in Ultrafiltration

Evaluation of Front-Face Fluorescence for Assessing Cyanobacteria Fouling in Ultrafiltration

Modulable 3D-printed plantibody-laden platform enabling microscale affinity extraction and ratiometric front-face fluorescence detection of microcystin-LR in marine waters

Solid-phase fluorescence: Reproducibility and comparison with the solution states

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