1995
DOI: 10.1111/j.1432-1033.1995.tb20389.x
|View full text |Cite
|
Sign up to set email alerts
|

Characterization of Activators and Inhibitors of Protein Kinase Cμ

Abstract: In order to investigate regulatory mechanisms and to identify potential substrates of a novel member of the protein kinase C (PKC) family, PKCp, specific antibodies have been raised against unique aminoand carboxy-terminal regions. PKCp kinase activity was studied upon immunoprecipitation from stably transfected cell lines as well as from the A549 carcinoma cell line expressing the endogeneous PKCp gene. Cell fractionation revealed that PKCp is predominantly found in the particulate fraction, suggesting an ass… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

11
201
1

Year Published

1996
1996
2021
2021

Publication Types

Select...
6

Relationship

2
4

Authors

Journals

citations
Cited by 157 publications
(213 citation statements)
references
References 35 publications
11
201
1
Order By: Relevance
“…Due to these properties PKCIa might have been classified as a member of the nPKC subgroup of the PKC family. However, PKC~t significantly differs in some structural features from the other PKC isotypes known so far [5]. With a sequence of 912 amino acids PKC~t is larger than the other PKCs.…”
Section: Introductionmentioning
confidence: 90%
See 4 more Smart Citations
“…Due to these properties PKCIa might have been classified as a member of the nPKC subgroup of the PKC family. However, PKC~t significantly differs in some structural features from the other PKC isotypes known so far [5]. With a sequence of 912 amino acids PKC~t is larger than the other PKCs.…”
Section: Introductionmentioning
confidence: 90%
“…After 30 min at 4°C the precipitated proteins were pelleted, redissolved in sample buffer, separated by SDS-polyacrylamide gel electrophoresis, and visualized by autoradiography of the gels. To analyze in vivo PKCI.t autophosphorylation NIH3T3 PKCI.t overexpressing transfectants were established essentially as previously described [5]. PKQt expression was induced by incubating 60% confluent growing transfectants 3 h in phosphate-free DMEM medium adding 1 ~tM CdC12 and 300 ~tCi 32p-labelled orthophosphate (Amersham) in a final volume of 2 ml.…”
Section: Autophosphorylation Of Pkcixmentioning
confidence: 99%
See 3 more Smart Citations