Characterization of adenosine receptors in the human bladder carcinoma T24 cell line

Phelps, P. Tara; Anthes, John C.; Correll, Craig C.

doi:10.1016/j.ejphar.2006.02.046

Cited by 20 publications

(21 citation statements)

References 35 publications

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“…However, in our study the selective adenosine A 2A receptor agonist CGS 21680 failed to stimulate cAMP production in UROtsa cells. In agreement with our data, Phelps et al [7] did neither detect cAMP elevation upon adenosine A 2A receptor activation in T24 bladder cells and nor any increase in intracellular Ca 2+ . These data may suggest that the adenosine A 2A receptor in human uroepithelial cells is functionally inactive or that cAMP-and Ca 2+ -independent signaling pathways are used for activation.…”

Section: Discussionsupporting

confidence: 82%

“…This is in line with previous studies conducted on T24 human bladder carcinoma cells [7] and A498 human kidney carcinoma cells [6] . Western blot analysis confirmed a high expression of the adenosine A 2B receptor and a lower expression of the A 2A receptor subtype.…”

Section: Discussionsupporting

confidence: 80%

“…The uroepithelial cells lining the human urinary tract express the adenosine A 1 , A 2A and A 2B receptor subtypes [6,7] but the role of these receptors in the mucosal inflammatory network is not understood. In other tissues, signaling via adenosine A 2A receptors inhibits inflammation [8] , in part by decreasing the release of proinflammatory cytokines [9,10] .…”

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confidence: 99%

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Effects of Adenosine A2A and A2B Receptor Activation on Signaling Pathways and Cytokine Production in Human Uroepithelial Cells

Säve

Persson

2010

Pharmacology

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Aims: Adenosine A_2A and A_2B receptor subtypes have both been implicated in the modulation of inflammation. We examined adenosine A_2A and A_2B receptor expression, signaling pathways and the effect of adenosine A_2A and A_2B receptor activation on the uropathogenic Escherichia coli (UPEC)-stimulated IL-8 response in human uroepithelial cells (UROtsa). Methods: Receptor expression was examined by RT-PCR and Western blot, and IL-8 production, intracellular cAMP levels and phosphoproteins were measured by ELISA, EIA and multiplex immunoassay, respectively. Results: The adenosine A₁, A_2A and A_2B receptor subtypes were detected in UROtsa cells. The adenosine A_2A receptor agonist CGS 21680 did not stimulate cAMP production but CREB phosphorylation was slightly increased. The adenosine A₂ receptor agonist CPCA induced a pronounced cAMP and CREB response. Furthermore, CGS 21680 but not CPCA decreased ERK 1/2 and STAT3 phosphorylation. UPEC infection stimulated the host IL-8 production but CPCA or CGS 21680 did not affect UPEC-evoked IL-8 production. Conclusions: Our data identified differences in signaling pathways evoked by adenosine A_2A and A_2B receptor activation. Activation of the adenosine A_2A receptor inhibited STAT3 and ERK 1/2 phosphorylation, while the cAMP-CREB pathway was induced by adenosine A_2B receptor activation. No anti- or proinflammatory effects were found for uroepithelial adenosine A_2A or A_2B receptors.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 80%

mentioning

confidence: 99%

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Effects of Adenosine A2A and A2B Receptor Activation on Signaling Pathways and Cytokine Production in Human Uroepithelial Cells

Säve

Persson

2010

Pharmacology

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“…In the human bladder T24 cell line, adenosine increased [Ca 2+ ] i and cAMP production as well as IL-8 secretion, via A 2B receptors [512]. It has been reported that A 2B receptor blockade slows the growth of bladder tumours [153].…”

Section: Bladder Cancermentioning

confidence: 99%

Purinergic signalling and cancer

Burnstock

Virgilio

2013

Purinergic Signalling

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Receptors for extracellular nucleotides are widely expressed by mammalian cells. They mediate a large array of responses ranging from growth stimulation to apoptosis, from chemotaxis to cell differentiation and from nociception to cytokine release, as well as neurotransmission. Pharma industry is involved in the development and clinical testing of drugs selectively targeting the different P1 nucleoside and P2 nucleotide receptor subtypes. As described in detail in the present review, P2 receptors are expressed by all tumours, in some cases to a very high level. Activation or inhibition of selected P2 receptor subtypes brings about cancer cell death or growth inhibition. The field has been largely neglected by current research in oncology, yet the evidence presented in this review, most of which is based on in vitro studies, although with a limited amount from in vivo experiments and human studies, warrants further efforts to explore the therapeutic potential of purinoceptor targeting in cancer.

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“…A 2B ARs are the low affinity adenosine receptors which are overexpressed in various tumors (Panjehpour et al, 2005, Li et al, 2005, Phelps et al, 2006. These receptors are involved in promotion of the tumor growth (Ryzhov et al, 2008).…”

Section: A 2b Adenosine Receptors and Melanomamentioning

confidence: 99%