Characterization of Aerobic Spore-Forming Bacteria Isolated From Raw Milk, Skim Milk Powder and UHT Milk

doi:10.21608/asejaiqjsae.2017.2348

Cited by 7 publications

(2 citation statements)

References 20 publications

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“…However, all the skim milk powder samples were positive for Total Viable bacteria with counts of a minimum of 20 CFU/g and a maximum of 7.00 × 10 2 CFU/g. This finding was nearly similar to data obtained by Yacoub et al (2017). Only 20.00% of the samples were positive for Total Staphylococci Count with a mean value of 0.03 × 10 2 ±0.02 × 10 2 CFU/g.…”

Section: Statistical Analysis Of the Compositional Parameters For The Examined Milk Powder Samplessupporting

confidence: 90%

Safety and quality aspects of whole and skimmed milk powders

Ibrahim

Saad

Hafiz

2021

Acta Sci Pol Technol Aliment

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Background. Nowadays, dried milk products are highly traded and consumed all over the world, so we aimed in this study to evaluate to what extent whole and skim milk powders are safe and comply with Egyptian standards. Materials and methods. Eighty samples of dried milk (50 whole milk powder and 30 skim milk powder) were gathered from several retailers and supermarkets for evaluation of their differing quality and safety parameters. Results. The most frequent off-flavors recovered from whole milk powder samples were cooked ones and, in the case of skim milk powder samples, flat ones. Five samples of whole milk powder were of fair quality and three samples of poor quality, according to the sensory evaluation. The compositional parameters, moisture, %, fat, %, protein, %, and acidity, %, were measured as mean values of 3.90 ±0.15, 26.90 ±0.19, 25.53 ±0.27, and 0.99 ±0.03% in the examined whole milk powder samples and 3.77 ±0.08, 1.11 ±0.05, 34.62 ±0.29, and 1.22 ±0.03% in the examined skimmed milk powder samples, respectively. These results were within the range of component requirements set by the Egyptian Standard (2014; ES: 1780/2014) for dried milk products. Also, the microbiological safety of the milk powder samples was analyzed by assessment of the total viable count, total yeast and mold count, Coliforms count, Enterobacteriaceae, E. coli, C. sakazakii, Staphylococcus aureus, Salmonella, and Listeria monocytogenes. Staphylococcus aureus was the most prevalent isolate (36.00% and 6.67%) followed by Enterobacteriaceae (20.00% and 3.33%), of whole and skim milk powder, respectively. Enterobacteriaceae isolates included Enterobacter cloacae ssp. Cloacae, and Pantoea spp., which were specified by traditional biochemical tests and Vitek2 system. All Enterobacteriaceae isolated spp. were resistant to cephalothin, neomycin, tobramycin and colistin sulphate, and sensitive to chloramphenicol, gentamycin and nalidixic acid. E. coli, C. sakazakii, Salmonella, and Listeria monocytogenes couldn't be isolated from all the tested samples. By using Inductive Coupled Plasma -Mass Spectrometer (ICP-MS), we could measure lead and mercury as mean values of 0.243 ±0.069 and 0.261 ±0.052 mg/kg for whole milk powder samples at a percentage of 68.00 and 34.00%, while for the skim milk powder samples they were 0.150 ±0.037, and 0.347 ±0.110 mg/kg at a percentage of 66.67 and 40.00%, respectively. Conclusion. Finally, thirty-four whole milk powder and twelve skimmed milk powder samples didn't comply with Egyptian standards, so it is necessary for authorities to put more attention on this and regular monitor it.

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Section: Statistical Analysis Of the Compositional Parameters For The Examined Milk Powder Samplessupporting

confidence: 90%

Safety and quality aspects of whole and skimmed milk powders

Ibrahim

Saad

Hafiz

2021

Acta Sci Pol Technol Aliment

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“…The higher maximum level of spore forming bacteria in the individual samples might be the reason for elevating the bulk milk samples. The high counts of aerobic spore-forming bacteria associated with raw milk increased the possibility of spoiling the final product and hence reducing its shelf life (Yacoub et al 2017). The results revealed the same mean value (3×10 3 cfu/ml) for the total aerobic spore count of milk samples collected from two study areas (Table 1).…”

Section: Discussionmentioning

confidence: 62%

Enumeration and characterization of aerobic bacillus species in cow’s milk from farms in Khartoum North, Sudan

Aseed¹,

Zubeir²

2020

VMPH

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This cross-sectional study was conducted with the objectives of determining the loads and types of spore-former bacteria from the genus Bacillus in cow's milk. One hundred samples of raw milk were randomly obtained from 20 dairy farms in Hillat Kuku and El seelait complexes in Khartoum North. Fifty samples from 10 dairy farms from each location were included (3 individual cows and 2 bulk milk). All collected milk samples were examined for enumeration of the spore forming bacteria and isolation and identification of Bacillus species. This study revealed that 45% of the examined milk samples showed prevalence of spore-forming bacteria. Moreover, the higher count was found in the bulk milk (5.2×10 3 cfu /ml) compared to the individual milk samples (3×10 3 cfu /ml). The 45 isolates found are belong to Bacillus spp. included B. cereus (15.5%), B. pantothenticus (8.8%), B. licheniformis (13.3%), B. mycoides (11.1%), B. coagulans (37.7%), B. megaterium (4.4%) and B. subtilis (8.8%). The rate of isolation for B. cereus were 24% and 10%, B. pantothenticus 8% and 8%, B. licheniformis 5% and 20%, B. mycoides 8% and 12%, B. coagulans 24% and 55%, B. megaterium 4% and 4%, and B. subtilis 4% and 13% from an individual and bulk samples, respectively. In conclusion, this study showed that almost half of milk samples produced in the dairy farms in the studied areas were harbouring a lot of Bacillus species with high load and occurrence in the bulk milk suggesting that extra sources of contamination might arise during storage. Hence the study suggested the need for improved hygienic practices at the farms level. Moreover, for quality assurance, regular control programs have to be applied for quality production and consumption of milk and its products in Sudan.

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The combined use of ¹H and 2D NMR‐based metabolomics and chemometrics for non‐targeted screening of biomarkers and identification of reconstituted milk

Cui

Zhu

et al. 2019

J Sci Food Agric

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BACKGROUND The illegal undeclared addition of reconstituted milk powder to ultra‐heat treated (UHT) milk to lower production costs is an example of economically motivated adulteration. This activity not only defrauds consumers but also places honest traders at a disadvantage, which could damage the reputation of milk producers and reduce the integrity of the markets. In this research, a non‐targeted analytical strategy that combines proton (1H) nuclear magnetic resonance (NMR) spectroscopy with a chemometrics data mining tool was developed for the authentication of bovine UHT milk. RESULTS Unsupervised principal component analysis was used to distinguish UHT and tap‐water‐reconstituted powdered milk. Partial least squares‐discriminant analysis (PLS‐DA) with R2(Y) and Q2 equal to 0.859 and 0.748, respectively, was used to differentiate UHT and reconstituted milk samples. Three compounds were selected as biomarkers to distinguish UHT and reconstituted milk and identified according to the standard NMR‐spectra database. Finally, a PLS‐DA model was established, according to the characteristic spectral bands, to identify UHT milk and reconstituted milk. CONCLUSION This procedure demonstrated the feasibility of using non‐targeted NMR profiling combined with chemometric analysis to combat mislabeling and fraudulent practices in milk production. © 2019 Society of Chemical Industry

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Characterization of Aerobic Spore-Forming Bacteria Isolated From Raw Milk, Skim Milk Powder and UHT Milk

Cited by 7 publications

References 20 publications

Safety and quality aspects of whole and skimmed milk powders

Safety and quality aspects of whole and skimmed milk powders

Enumeration and characterization of aerobic bacillus species in cow’s milk from farms in Khartoum North, Sudan

The combined use of ¹H and 2D NMR‐based metabolomics and chemometrics for non‐targeted screening of biomarkers and identification of reconstituted milk

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Characterization of Aerobic Spore-Forming Bacteria Isolated From Raw Milk, Skim Milk Powder and UHT Milk

Cited by 7 publications

References 20 publications

Safety and quality aspects of whole and skimmed milk powders

Safety and quality aspects of whole and skimmed milk powders

Enumeration and characterization of aerobic bacillus species in cow’s milk from farms in Khartoum North, Sudan

The combined use of 1H and 2D NMR‐based metabolomics and chemometrics for non‐targeted screening of biomarkers and identification of reconstituted milk

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Product

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The combined use of ¹H and 2D NMR‐based metabolomics and chemometrics for non‐targeted screening of biomarkers and identification of reconstituted milk