Characterization of aeromonads isolated from the river Narmada, India

Sharma, Anjana; Dubey, Nidhi; Sharan, Bandana

doi:10.1016/j.ijheh.2005.03.007

Cited by 22 publications

(15 citation statements)

References 18 publications

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“…Similar results have been described by other authors (Smoot & Pearson 1997;Bahlaoui et al 1997;Maalej et al 2003;Sharma et al 2005). The absence of correlation can be explained by differences in characteristics of each group of organisms, such as occurrence of enteric organisms, in the case of E. coli.…”

Section: ; Martin-carnahan and Joseph 2005)supporting

confidence: 89%

Dynamics of Aeromonas species isolated from wastewater treatment system

Martone-Rocha

Piveli

Matté

et al. 2010

Journal of Water and Health

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Aeromonas are widely distributed in the aquatic environment, and are considered to be emerging organisms that can produce a series of virulence factors. The present study was carried out in a sanitary sewage stabilization pond treatment system, located in Lins, State of Sã o Paulo, Brazil.Most probable number was applied for estimation of the genus Aeromonas. Colony isolation was carried out on blood agar ampicillin and confirmed by biochemical characterization. Aeromonas species were isolated in 72.4% of influent samples, and in 55.2 and 48.3% of effluent from anaerobic and facultative lagoons, respectively. Thirteen Aeromonas species were isolated, representing most of the recognized species of these organisms. Even though it was possible to observe a tendency of decrease, total elimination of these organisms from the studied system was not achieved. Understanding of the pathogenic organism's dynamics in wastewater treatment systems with a reuse potential is especially important because of the risk it represents.

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Section: ; Martin-carnahan and Joseph 2005)supporting

confidence: 89%

Dynamics of Aeromonas species isolated from wastewater treatment system

Martone-Rocha

Piveli

Matté

et al. 2010

Journal of Water and Health

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“…RAPD or AP-PCR (arbitrarily primed polymerase chain reaction) is a DNA fi ngerprinting technique, which is one of the most sensitive and effi cient method for distinguishing different strains of a species [19]. RAPD was choosen as it has been reported to be an effective subtyping method for several other species, namely Listeria, Yersinia [20], Staphylococcus [21], Streptococcus [22] and Aeromonas [23]. Antimicrobial resistance in enteric pathogens is of great signifi cance in the developing world, where the rate of diarrheal diseases is highest.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and genotypic characterization of Shigella spp. with reference to its virulence genes and antibiogram analysis from river Narmada

2009

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Water samples of the river Narmada from the source to the mouth were analyzed for the presence of shigellae and the Shigella isolates from 180 water samples were characterized by biotyping, serotyping and molecular typing. Out of all the 40 isolates, 23 were identified as S. flexneri, 10 as S. sonnei and 7 as S. dysenteriae. Serotyping was found to be the better identification method than biotyping. In the present investigation, amplified ribosomal DNA restriction analysis (ARDRA) with a probe complementary to 16S rRNA was performed. Repeated ARDRA analysis established the similarities between the isolates and thus suggested ARDRA as authentic and precise detection protocol. The isolates were also analyzed for the presence of virulence genes including ipaBCD, ipaH and stx1. All the 40 isolates of Shigella were found to be positive for the ipaH gene. The plasmid encoded invasion-associated genes ipaBCD were present only in S. flexneri and the stx1 gene was found only in S. dysenteriae. This study demonstrated the existence of Shigella in the river Narmada and the dispersion of different virulence genes among the isolates, which appear to constitute an environmental reservoir of Shigella-specific virulence genes.

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“…In rural communities, untreated surface water from rivers, dams, and streams is directly used for drinking and other domestic purposes [5]. These unprotected water sources can be contaminated with microbes through rainfall run-off and agricultural inputs, mixing with sewage effluents and faeces from wild life [6, 7], which render them unacceptable for human consumption. Faecal coliforms, Aeromonas and Pseudomonas, are used as indicators of faecal contamination in water [8] and the presence of these pathogens may have severe health implications on consumers especially those that are immunocompromised [5, 9, 10].…”

Section: Introductionmentioning

confidence: 99%

Isolation of Environmental Bacteria from Surface and Drinking Water in Mafikeng, South Africa, and Characterization Using Their Antibiotic Resistance Profiles

Mulamattathil

Bezuidenhout

Mbewe

et al. 2014

Journal of Pathogens

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The aim of this study was to isolate and identify environmental bacteria from various raw water sources as well as the drinking water distributions system in Mafikeng, South Africa, and to determine their antibiotic resistance profiles. Water samples from five different sites (raw and drinking water) were analysed for the presence of faecal indicator bacteria as well as Aeromonas and Pseudomonas species. Faecal and total coliforms were detected in summer in the treated water samples from the Modimola dam and in the mixed water samples, with Pseudomonas spp. being the most prevalent organism. The most prevalent multiple antibiotic resistance phenotype observed was KF-AP-C-E-OT-K-TM-A. All organisms tested were resistant to erythromycin, trimethoprim, and amoxicillin. All isolates were susceptible to ciprofloxacin and faecal coliforms and Pseudomonas spp. to neomycin and streptomycin. Cluster analysis based on inhibition zone diameter data suggests that the isolates had similar chemical exposure histories. Isolates were identified using gyrB, toxA, ecfX, aerA, and hylH gene fragments and gyrB, ecfX, and hylH fragments were amplified. These results demonstrate that (i) the drinking water from Mafikeng contains various bacterial species and at times faecal and total coliforms. (ii) The various bacteria are resistant to various classes of antibiotics.

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Characterization of aeromonads isolated from the river Narmada, India

Cited by 22 publications

References 18 publications

Dynamics of Aeromonas species isolated from wastewater treatment system

Dynamics of Aeromonas species isolated from wastewater treatment system

Phenotypic and genotypic characterization of Shigella spp. with reference to its virulence genes and antibiogram analysis from river Narmada

Isolation of Environmental Bacteria from Surface and Drinking Water in Mafikeng, South Africa, and Characterization Using Their Antibiotic Resistance Profiles

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