Characterization of Afp1, an antifreeze protein from the psychrophilic yeast Glaciozyma antarctica PI12

Hashim, Nik Mohd Hazrul Nik; Bharudin, Izwan; Law, Douglas; Higa, Sakura; Bakar, Farah Diba Abu; Nathan, Sheila; Rabu, Amir; Kawahara, Hidehisa; Illias, Rosli Md.; Najimudin, Nazalan; Mahadi, Nor Muhammad; Murad, Abdul Munir Abdul

doi:10.1007/s00792-012-0494-4

Cited by 67 publications

(46 citation statements)

References 48 publications

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“…Currently, only AFP-1 gene sequence has been successfully characterized and expressed from the yeast [22]. The large antifreeze protein (AFP) from this antarctica yeast shows very low homology as compared to other AFP genes [23]. Interestingly, the predicted three-dimensional structure of AFP-1 sequence shows the presence of several α-helical conformations in its structure.…”

Section: Resultsmentioning

confidence: 99%

“…The irruptive of ice recrystallization phase was occurred, generating a very fine of ice nuclei with different size in different sample. A reduce number and growth of ice crystals size in peptide solution after 3 hours incubation period would be indicative of the peptide efficiency in inhibiting ice crystal growth [23]. The sample microscopic images were captured at the end of incubation period at 100x magnification.…”

Section: Peptide Designmentioning

confidence: 99%

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Tailoring Peptidomimetics Antifreeze Protein From Exotic Antarctic Marine

Rahman¹,

AidaAsmawi²,

Abdulmalek³

et al. 2016

MJAS

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Antifreeze proteins (AFPs) are synthesized by various cold-adapted organisms to enable them to survive in subzero environment. The unique role of AFPs recently attracted enormous interest to develop them as commercial products. In this work, we have studied the antifreeze activity of short helical protein fragments (peptides) instead of the entire antifreeze protein of Antarctic yeast Glaciozyma antarctica. Several short peptide segments were designed according to amino acid sequence of helical region of AFP-1 G.antarctica, which are assumed to be involved in its antifreeze activity. We have demonstrated that short peptide segments derived from yeast AFP possess antifreeze activity and result in modification of the ice crystals growth rates and habits. This strategy has enabled the preparation of short AFP with high antifreeze activity in large amount of quantities at a low cost further opens the chance of developing the commercial potentials of AFPs. Keywords: Antarctic yeast, antifreeze protein (AFP), Glaciozyma antarctica, ice recrystallization inhibition (IRI) AbstrakProtein antibeku (AFPs) disintesis oleh pelbagai adaptasi-sejuk organisma untuk membolehkan ia bertahan di persekitaran suhu bawah takat beku. Peranan unik AFPs telah menarik minat yang besar bagi tujuan produk komersil. Di dalam kajian ini, kami telah mengkaji aktiviti protein antibeku berlingkar yang pendek (peptida) daripada protein antibeku Antartika yis Glaciozyma antarctica. Beberapa segmen peptida pendek direka mengikut jujukan asid amino berlingkar AFP-1 G.antarctica, yang dianggap terlibat dalam aktiviti antibeku. Kami telah menunjukkan bahawa segmen -segmen peptida pendek yang diperolehi daripada yis AFP mempunyai aktiviti antibeku dan menyebabkan pengubahsuaian kadar pertumbuhan dan tabiat hablur ais. Strategi ini telah membolehkan penyediaan AFP pendek dengan aktiviti antibeku yang tinggi dalam jumlah kuantiti besar pada kos yang rendah seterusnya membuka peluang untuk membangunkan potensi komersial AFPs.Kata kunci: yis Antartika, protein antibeku (AFP), Glaciozyma antarctica, perencatan penghabluran ais (IRI) IntroductionThe effect of sub-zero temperatures on organisms can cause extensive damage due to the presence of ice crystals. If freezing persists, intracellular water passes into extracellular spaces and leads to cell dehydration. Despite this,

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Section: Resultsmentioning

confidence: 99%

Section: Peptide Designmentioning

confidence: 99%

Tailoring Peptidomimetics Antifreeze Protein From Exotic Antarctic Marine

Rahman¹,

AidaAsmawi²,

Abdulmalek³

et al. 2016

MJAS

View full text Add to dashboard Cite

show abstract

“…High TH and inhibition of ice recrystallization properties were detected in culture filtrates of the psychrophilic yeast Glaciozyma antarctica and, according to genomic data, a cDNA encoding a probable 177-residue AFP was found. This cDNA was expressed in Escherichia coli, obtaining a recombinant protein with antifreeze properties (Hashim et al 2013). The Arctic yeast Leucosporidium sp.…”

Section: Antifreeze Proteinsmentioning

confidence: 99%

Physiological adaptations of yeasts living in cold environments and their potential applications

Alcaı́no

Cifuentes

Baeza

2015

World J Microbiol Biotechnol

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Yeasts, widely distributed across the Earth, have successfully colonized cold environments despite their adverse conditions for life. Lower eukaryotes play important ecological roles, contributing to nutrient recycling and organic matter mineralization. Yeasts have developed physiological adaptations to optimize their metabolism in low-temperature environments, which affect the rates of biochemical reactions and membrane fluidity. Decreased saturation of fatty acids helps maintain membrane fluidity at low temperatures and the production of compounds that inhibit ice crystallization, such as antifreeze proteins, helps microorganisms survive at temperatures around the freezing point of water. Furthermore, the production of hydrolytic extracellular enzymes active at low temperatures allows consumption of available carbon sources. Beyond their ecological importance, interest in psychrophilic yeasts has increased because of their biotechnological potential and industrial uses. Long-chain polyunsaturated fatty acids have beneficial effects on human health, and antifreeze proteins are attractive for food industries to maintain texture in food preserved at low temperatures. Furthermore, extracellular cold-active enzymes display unusual substrate specificities with higher catalytic efficiency at low temperatures than their mesophilic counterparts, making them attractive for industrial processes requiring high enzymatic activity at low temperatures. In this minireview, we describe the physiological adaptations of several psychrophilic yeasts and their possible biotechnological applications.

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“…Psychrophilic micoorganisms have developed several strategies to overcome threats to their metabolic processes. These strategies include the maintenance of high levels of unsaturated and branched fatty acids to maintain the membrane permeability [3], production of cold active enzymes that have high flexibility [4] and the production of unique proteins such as ice nucleating protein (INP) and antifreeze protein (AFP) [5]. Glaciozyma antarctica is psychrophilic yeasts that able to grow at low temperature with optimum growth temperatures is 12°C [6].…”

Section: Introductionmentioning

confidence: 99%

Extraction of intracellular protein from Glaciozyma antarctica for proteomics analysis

Faizura

Farahayu

Faizal

et al. 2013

AIP Conference Proceedings

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Abstract. Two preparation methods of crude extracts of psychrophilic yeast Glaciozyma antarctica were compared in order to obtain a good recovery of intracellular proteins. Extraction with mechanical procedures using sonication was found to be more effective for obtaining good yield compare to alkaline treatment method. The procedure is simple, rapid, and produce better yield. A total of 52 proteins were identified by combining both extraction methods. Most of the proteins identified in this study involves in the metabolic process including glycolysis pathway, pentose phosphate pathway, pyruyate decarboxylation and also urea cyle. Several chaperons were identified including probable cpr1-cyclophilin (peptidylprolyl isomerase), macrolide-binding protein fkbp12 and heat shock proteins which were postulate to accelerate proper protein folding. Characteristic of the fundamental cellular processes inferred from the expressedproteome highlight the evolutionary and functional complexity existing in this domain of life.

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Characterization of Afp1, an antifreeze protein from the psychrophilic yeast Glaciozyma antarctica PI12

Cited by 67 publications

References 48 publications

Tailoring Peptidomimetics Antifreeze Protein From Exotic Antarctic Marine

Tailoring Peptidomimetics Antifreeze Protein From Exotic Antarctic Marine

Physiological adaptations of yeasts living in cold environments and their potential applications

Extraction of intracellular protein from Glaciozyma antarctica for proteomics analysis

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