Characterization of aldosterone binding sites in circulating human mononuclear leukocytes

Armanini, Decio; Strasser, Th.; Weber, Peter

doi:10.1152/ajpendo.1985.248.3.e388

Cited by 82 publications

(59 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As mentioned above, MR are also present in non-epithelial cells/ tissues such as the hippocampus (McEwen & Wallach 1973), macrophages (Armanini et al 1985a, Lim et al 2007, VSMC, placenta and heart (Pearce & Funder 1988, Lombes et al 1992. In the absence of the aldosterone specificity-conferring enzyme 11bHSD2, MR are overwhelmingly occupied by glucocorticoids.…”

Section: Mrs Macrophages and Diseasementioning

confidence: 97%

Corticosteroid receptors, macrophages and cardiovascular disease

Rickard¹,

Young²

2009

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

The mineralocorticoid receptor (MR) and glucocorticoid receptor are ligand-activated transcription factors that have important physiological and pathophysiological actions in a broad range of cell types including monocytes and macrophages. While the glucocorticoids cortisol and corticosterone have well-described anti-inflammatory actions on both recruited and tissue resident macrophages, a role for the mineralocorticoid aldosterone in these cells is largely undefined. Emerging evidence, however, suggests that MR signalling may promote pro-inflammatory effects. This review will discuss the current understanding of the role of corticosteroid receptors in macrophages and their effect on diseases involving inflammation, with a particular focus on cardiovascular disease.

show abstract

Section: Mrs Macrophages and Diseasementioning

confidence: 97%

Corticosteroid receptors, macrophages and cardiovascular disease

Rickard¹,

Young²

2009

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

show abstract

“…ALDO binds to a single class of cytosolic receptors in these cells. 37 In patients with either PAL or renin-dependent, secondary aldosteronism (SAL),ALDO binding sites are reduced by 50%; following surgical removal of adenomatous adrenal tissue, receptor binding in PBMC is normalised. 38,39 ALDO promotes the efflux of Mg 2+ from cultured human lymphocytes in exchange for Na + via receptor-ligand binding.…”

Section: Non-epithelial Cells Aldo Regulates Mgmentioning

confidence: 99%

Toward a broader understanding of aldosterone in congestive heart failure

Weber

Sun

Wodi

et al. 2003

J Renin Angiotensin Aldosterone Syst

View full text Add to dashboard Cite

Discovered some 50 years ago, aldosterone (ALDO) has come to be recognised as a mineralocorticoid hormone with well-known endocrine properties in epithelial cells that contribute to the pathophysiology of congestive heart failure. This includes Na + in such non-epithelial cells as peripheral blood mononuclear cells; its influence on endothelial cell function; and its central actions that involve regulation of cerebrospinal fluid composition produced by epithelial cells of the choroid plexus, activity of the hypothalamic paraventricular nucleus involved in Na + appetite, Na + and H 2 O excretion and sympathetic nerve activity, and the regulation of TNF-α production from central and/or peripheral sources. Extra-adrenal steroidogenesis and auto/paracrine properties of ALDO generated de novo in the cardiovasculature are now under investigation and preliminary findings suggest they contribute to tissue repair. The past decade has witnessed a revival of interest in this steroid molecule. In years to come, an even broader understanding of ALDO's contribution to the pathophysiology of congestive heart failure will undoubtedly emerge.

show abstract

“…Several investigators reported that not only Ang II but also aldosterone induced the activation of nuclear factor (NF)-κB through either AT1R (9,11,24,25) or MR (15,26). NF-κB enhances gene expressions involved in inflammation with subsequent cytokine production such as monocyte chemoattractant protein-1 (MCP-1) (27) and tumor necrosis factor-α (TNF-α) (11) in human PBMCs.…”

Section: Introductionmentioning

confidence: 99%

Aldosterone Synthesis and Cytokine Production in Human Peripheral Blood Mononuclear Cells

et al. 2006

View full text Add to dashboard Cite

Abstract. Previously, we reported that spironolactone reduced cytokine production in cultured human peripheral blood mononuclear cells (PBMCs) with angiotensin (Ang) II stimulation. To address the mechanisms underlying this effect, we examined the contribution of aldosterone to cytokine production in cultured human PBMCs with Ang II stimulation. PBMCs expressed the messenger RNA (mRNA) of Ang II type 1 receptor (AT1R) and mineralocorticoid receptor (MR) both spontaneously and after Ang II stimulation, but expressed Ang II type 2 receptor (AT2R) under neither condition. After 24 h of incubation, exogenous Ang II induced the expression of CYP11B2 (a key enzyme of aldosterone synthesis) mRNA and caused aldosterone synthesis. CV-11974 (an AT1R antagonist) reduced Ang II-induced aldosterone synthesis, whereas PD-123319 (an AT2R antagonist) had no effect. The concentration of aldosterone peaked earlier than those of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α). After 48 h of incubation (under the influence of synthesized aldosterone), CV-11974 and spironolactone significantly reduced the Ang II-enhanced production of MCP-1 and TNF-α, whereas PD-123319 also had no effect. In conclusion, Ang II induces aldosterone synthesis through AT1R and enhances cytokine production through an AT1R-dependent mechanism and, at least partly, through a MR-dependent mechanism in human PBMCs.

show abstract

Characterization of aldosterone binding sites in circulating human mononuclear leukocytes

Cited by 82 publications

References 9 publications

Corticosteroid receptors, macrophages and cardiovascular disease

Corticosteroid receptors, macrophages and cardiovascular disease

Toward a broader understanding of aldosterone in congestive heart failure

Aldosterone Synthesis and Cytokine Production in Human Peripheral Blood Mononuclear Cells

Contact Info

Product

Resources

About