Characterization of alginate/chitosan-based nanoparticles and mathematical modeling of their SpBMP-9 release inducing neuronal differentiation of human SH-SY5Y cells

Lauzon, Marc-Antoine; Marcos, Bernard; Faucheux, Nathalie

doi:10.1016/j.carbpol.2017.11.075

Cited by 26 publications

(31 citation statements)

References 48 publications

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“…Alg/Chit NPs were synthesized using an ionotropic gelation method as previously reported [10]. Briefly, a CaCl 2 solution (18 mM) with a final ratio Alg: CaCl 2 of 5:1 (w/w) was added dropwise to a solution of Alg (1 mg/mL, pH 5.1) under vigorous stirring.…”

Section: Nanoparticle Synthesismentioning

confidence: 99%

“…After 168 h of incubation, Alg/Chit NPs were dissolved into Tris (10 mM)-EDTA (1 mM) buffer for 20 min at room temperature and the remaining concentration of CXCL12-AF647 was determined by fluorescence quantification. Mathematical modeling was performed, as previously reported [10]. Briefly, the mathematical framework considers the following assumptions:…”

Section: Release Kineticsmentioning

confidence: 99%

“…It is obtained after partial deacetylation of the chitin, a poly (β-(1→4)-N-acetyl-D-glucosamine), found in crustacean shells (crabs and prawn), the cocoon of insects, and fungi cell wall [6][7][8]. However, due to its cationic and alkaline nature, chitosan must be modified to bear carboxyl groups or combined with negatively charged polymers such as alginate to form NPs that can better entrap positively charged molecules [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…However, the kinetics of CXCL12 released from NPs delivery systems are rarely characterized using mathematical modeling. Such an experimental approach is important not only to better understand the phenomena that control the chemokine delivery but also help to optimize and calibrate the delivery to the desired parameters [10,27]. For example, we have previously developed a composite NPs delivery system made of alginate (Alg) and chitosan (Chit) to encapsulate a positively charged peptide derived from bone morphogenetic protein-9 (SpBMP-9, 23 residues) for brain application.…”

Section: Introductionmentioning

confidence: 99%

“…For example, we have previously developed a composite NPs delivery system made of alginate (Alg) and chitosan (Chit) to encapsulate a positively charged peptide derived from bone morphogenetic protein-9 (SpBMP-9, 23 residues) for brain application. We found that the presence of Alg (negatively charged), besides being essential for the synthesis of NPs by ionotropic gelation, could promote high encapsulation efficiency, strongly enhance the interactions with the peptide, and therefore allow its sustained release for up to 7 days [10]. In the same study, we also developed a mechanistic mathematical framework, adapted to NPs delivery system, to study the release kinetics.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Mathematical Modeling of Alginate/Chitosan-Based Nanoparticles Releasing the Chemokine CXCL12 to Attract Glioblastoma Cells

et al. 2020

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Chitosan (Chit) currently used to prepare nanoparticles (NPs) for brain application can be complexed with negatively charged polymers such as alginate (Alg) to better entrap positively charged molecules such as CXCL12. A sustained CXCL12 gradient created by a delivery system can be used, as a therapeutic approach, to control the migration of cancerous cells infiltrated in peri-tumoral tissues similar to those of glioblastoma multiforme (GBM). For this purpose, we prepared Alg/Chit NPs entrapping CXCL12 and characterized them. We demonstrated that Alg/Chit NPs, with an average size of ~250 nm, entrapped CXCL12 with ~98% efficiency for initial mass loadings varying from 0.372 to 1.490 µg/mg NPs. The release kinetic profiles of CXCL12 were dependent on the initial mass loading, and the released chemokine from NPs after seven days reached 12.6%, 32.3%, and 59.9% of cumulative release for initial contents of 0.372, 0.744, and 1.490 µg CXCL12/mg NPs, respectively. Mathematical modeling of released kinetics showed a predominant diffusive process with strong interactions between Alg and CXCL12. The CXCL12-NPs were not toxic and did not promote F98 GBM cell proliferation, while the released CXCL12 kept its chemotaxis effect. Thus, we developed an efficient and tunable CXCL12 delivery system as a promising therapeutic strategy that aims to be injected into a hydrogel used to fill the cavity after surgical tumor resection. This system will be used to attract infiltrated GBM cells prior to their elimination by conventional treatment without affecting a large zone of healthy brain tissue.

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Section: Nanoparticle Synthesismentioning

confidence: 99%

Section: Release Kineticsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization and Mathematical Modeling of Alginate/Chitosan-Based Nanoparticles Releasing the Chemokine CXCL12 to Attract Glioblastoma Cells

et al. 2020

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Alzheimer's Disease: Status of Low‐Dimensional Nanotherapeutic Materials

Huang,

Liao,

et al. 2023

Adv Funct Materials

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Alzheimer's disease (AD) is a chronic progressive neurodegenerative disease that constitutes the third most common cause of death among elderly individuals. AD patients suffer from behavioral problems regularly, like memory loss, thinking deficits, and cognitive disorders. Currently, the incidence rate of AD has been on the rise worldwide. Therefore, it is imperative to develop effective strategies for the treatment and prevention of AD. The inherent characteristics of low‐dimensional nanomaterials, primarily their smaller size and surface structure, make them well‐suited for fulfilling the essential requirements of therapeutic drug design. Consequently, these nanomaterials offer a highly appealing and alternative approach to treating AD. Here, the low‐dimensional nanomaterials used in the treatment of AD to provide new ideas, methods, and comprehensive perspectives for the management of AD are reviewed. This review will advance the comprehensive understanding of the potential of low‐dimensional nanomaterials in the field of neuro medicine, which also will have a positive impact on future research.

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An Up‐to‐Date Review on Alginate Nanoparticles and Nanofibers for Biomedical and Pharmaceutical Applications

Dodero

Alberti

Gaggero

et al. 2021

Adv Materials Inter

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along with other polysaccharides (e.g., celluloses, chitosan, etc.), is indeed considered an extremely promising material in the viewpoint of circular economy and in substituting petroleum-derived polymers. [3] Among others, one of the reasons for alginate success is its unique capability to bind different cations leading to stable and tailor-made hydrogels. [4] Owing to its biocompatibility, biodegradability, and nontoxicity, in the past decades alginate has been vastly explored for drug and gene delivery, tissue engineering, and wound healing applications. [5] In this sense, the recent nanotechnological advances have allowed the fabrication and exploitation of alginate-based nanostructured materials with completely new capabilities. Thereby, it is not surprising that nowadays a broad variety of alginate-based nanomaterials with various shapes, sizes, and compositions are prepared via different approaches, including controlled gelation, electrospinning and electrospraying, self-assembly, phase-separation, and micro fluidics., All these nanostructures hold the potential to interact with living organisms much more efficiently with respect to bulk systems, hence leading to specific functionalities at the same time avoiding the occurrence of toxicity issues. [6][7][8] Despite the use of alginate-based nanomaterials has been reviewed in the past, the focus has been commonly pointed to their generic properties and applications. Conversely, this review attempts to provide a more specific and comprehensive summary limited to the most recent advances in the fabrication and use of two of the most common and promising alginatebased nanomaterials, namely nanoparticles (NPs) and electrospun nanofibers (NFs), with a focus on biomedical and pharmaceutical applications.

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Characterization of alginate/chitosan-based nanoparticles and mathematical modeling of their SpBMP-9 release inducing neuronal differentiation of human SH-SY5Y cells

Cited by 26 publications

References 48 publications

Characterization and Mathematical Modeling of Alginate/Chitosan-Based Nanoparticles Releasing the Chemokine CXCL12 to Attract Glioblastoma Cells

Characterization and Mathematical Modeling of Alginate/Chitosan-Based Nanoparticles Releasing the Chemokine CXCL12 to Attract Glioblastoma Cells

Alzheimer's Disease: Status of Low‐Dimensional Nanotherapeutic Materials

An Up‐to‐Date Review on Alginate Nanoparticles and Nanofibers for Biomedical and Pharmaceutical Applications

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