Characterization of alkaline lipase from Fusarium oxysporum and the effect of different surfactants and detergents on the enzyme activity

Prazeres, Janaina Nicanuzia dos; Cruz, Juliana Aparecida Bortollotti; Pastore, Gláucia Maria

doi:10.1590/s1517-83822006000400019

Cited by 96 publications

(77 citation statements)

References 16 publications

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“…Tween 20 and 80 had inhibitory effects (54.7-31.4%) on the lipolytic activities at all levels Table 5. These results were similar to those of Prazeres et al (2006) responded to the added amino acid. Glutamic acid had a maximum inhibitory effect (29.4%).…”

Section: Response Of Some Additivessupporting

confidence: 86%

Optimization of lipase synthesis by Mucor racemosus - Production in a triple impeller bioreactor

Nadia¹,

Nehad²,

El-Sayed³

et al. 2010

MJM

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Fungal cultures were screened for their ability to produce extracellular lipase through different incubation periods, in spite of some Aspergillus niger gave highest lipolytic activities, Mucor racemosus was selected in this study due to its non-pathogenicity. Optimum lipase activity was obtained after 3 days incubation period at 35 °C using submerged culture technique. Using a modified complex medium (3% peptone; 0.2% KH2PO4; 0.05% KCl; 0.05% MgSO4 . 7H2O; 1% glucose; 1% olive oil and 0.01 M barium acetate as additives; pH was adjusted to 7.0 using 0.2 M phosphate buffer) stimulated M. racemosus lipase by 114.3%. The production of lipolytic enzyme by M. racemosus was up-scaled to 5 L stirred-tank bioreactor. Under optimized conditions, lipase and specific enzyme activities were improved by 2 folds (1211 U/mL and 28 U/mg protein) as compared to shake flask studies.

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Section: Response Of Some Additivessupporting

confidence: 86%

Optimization of lipase synthesis by Mucor racemosus - Production in a triple impeller bioreactor

Nadia¹,

Nehad²,

El-Sayed³

et al. 2010

MJM

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“…On other hand, some authors obtained lipase activity only after 5 days of cultivation (ABBAS et al, 2002;ACIKEL et al, 2011;RAPP, 1995). Investigations of the carbon source effects have been done in order to find good lipase inducers and enhance lipase production (PRAZERES et al, 2006;ANDRADE et al, 2013). RAPP et al (1995) mentioned the use of olive oil added to glucose to produce lipases by F. oxysporum.…”

Section: Transesterification Screeningmentioning

confidence: 99%

“…Fusarium oxyporum is well-known specie as good lipase producer (PRAZERES et al, 2006) and improvements on its production could be an important tool to optimize its application (KAUSHIK et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

A Useful Methodology to Select Lipase-Catalyzed Transesterification Aiming Biodiesel Application

Facchini

Vici

Pereira

et al. 2016

Revista Brasileira De Engenharia De Biossistemas

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The application of lipases in various fields has been notably increased in the last few decades and qualitative/quantitative improvements need to be done. However, many methodologies of screening are described in order to find a good lipase producer and statistical optimization is a necessary tool to improve lipase production. In this work, an isolation of filamentous fungi lipase producers and a transesterification capacity screening was evaluated. Four fungi were chosen to the transesterification reaction assays and the best fungus selected was submitted to a submerged fermentation. Parameters of the culture medium were optimized using response surface methodology. Selected liquid medium was SR at 30 °C, 72 h, 100 rpm. Corn oil was the best carbon source and together with Tween 80 increased two-fold the lipase activity. After the experimental design, the new medium optimized were 3.5-fold higher than the original liquid medium and was composed by 0.5% corn oil, 0.012% MgSO 4 .7H 2 O, 0.015% KH 2 PO 4 , 0.05% NH 4 H 2 PO 4 . Hence, the lipase produced proved its transesterification capacity and can be used for biodiesel production. Keywords: Lipase; Transesterification; Biodiesel UMA METODOLOGIA ÚTIL PARA SELECIONAR LIPASES COM ATIVIDADE DE TRANSESTERIFICAÇÃO VISANDO APLICAÇÃO NA PRODUÇÃO DE BIODIESEL RESUMOA aplicação de lipases em vários campos tem aumentado notavelmente nas últimas décadas e melhorias qualitativas/quantitativas precisam ser feitas. Entretanto, a fim de encontrar um bom produtor de lipase muitas metodologias de seleção são descritas, além disso, a otimização estatística é uma ferramenta necessária para melhorar a produção de lipases. Este trabalho apresenta o isolamento de fungos filamentosos produtores de lipase e a avaliação da capacidade de transesterificação dessas enzimas. Quatro fungos foram selecionados para os ensaios de transesterificação e o melhor fungo isolado foi submetido a fermentação submersa. Parâmetros do meio de cultivo foram otimizados usando metodologia de superfície de resposta. O meio de cultivo líquido selecionado foi o SR, a 30 °C, 72 h e 100 rpm. Óleo de milho foi a melhor fonte de carbono e, quando adicionado de Tween 80 aumentou duas vezes a atividade de lipase. Após o planejamento experimental, o novo meio de cultivo otimizado, composto por * polizeli@ffclrp.usp.br Brazilian Journal of Biosystems Engineering v. 10(1): 01-13, 2016 2 óleo de milho 0,5%, extrato de levedura 0,45%, peptona 0,2%, MgSO 4 .7H 2 O 0,012%, KH 2 PO 4 0,015% e NH 4 H 2 PO 4 0,05%, foi 3,5 vezes melhor que o original. Dessa forma, a lipase produzida demonstrou capacidade de transesterificação e pode ser utilizada para a produção de biodiesel.

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“…Lipase activity assay was carried out by the titrimetric method as described by [10]. The substrate used was 25% olive oil emulsion in 7% Arabic gum solution.…”

Section: Lipase Activity Assaymentioning

confidence: 99%

“…Effect of surfactants (SDS and Tween 80) and commercial detergents (Rinso, Attack, and SoKlin) on lipase activity were determined according to [10]. 250 μL of enzyme solution was preincubated in 250 μL of surfactants or commercial detergents (solution of 0.1 and 1.0% w/v) for 30 minutes at 30 °C.…”

Section: Lipase Activity Assaymentioning

confidence: 99%

Partial purification and biochemical characterization of extracellular lipase from Azospirillum sp. JG3 bacteria

Lestari¹,

Raharjo²,

Matsjeh³

et al. 2016

AIP Conference Proceedings

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Abstract. An extracellular lipase from Azospirillum sp.JG3 bacteria was partially purified by ammonium sulfate fractionation. Production of crude extract lipase in Nutrient Broth medium fortified with 1 % olive oil as inducer, fractionation of lipase using ammonium sulfate, and characterization of the highest specific activity of lipase fraction have been achieved in this study. The ammonium sulfate lipase fraction which has highest specific activity is F60 with its value is 12,283.33 U/mg protein. The optimum lipase activity of F60 was achieved at 40° C and pH 7.0. The addition of 1.0 mM EDTA strongly decreased its activity. It considered that this lipase was a metalloenzyme. The addition of 1.0 mM Ca 2+ solution showed that this metal ion increased lipase activity, it indicated that Ca 2+ ion is an activator of this lipase. Whereas addition of Mg 2+ , Zn 2+ , Co 2+ , and Cu 2+ metal ions decreased its activity. Effect of surfactants and commercial detergents addition towards lipase activity showed that all treatments decreased the activity.

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Characterization of alkaline lipase from Fusarium oxysporum and the effect of different surfactants and detergents on the enzyme activity

Cited by 96 publications

References 16 publications

Optimization of lipase synthesis by Mucor racemosus - Production in a triple impeller bioreactor

Optimization of lipase synthesis by Mucor racemosus - Production in a triple impeller bioreactor

A Useful Methodology to Select Lipase-Catalyzed Transesterification Aiming Biodiesel Application

Partial purification and biochemical characterization of extracellular lipase from Azospirillum sp. JG3 bacteria

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