Characterization of Alkaline Phosphatase-Positive and -Negative Cells Isolated from Human Periodontal Ligament Cells

Tsurumi, Ayuko; Kobayashi, Makoto; Murayama, Ryo-ichiro; Usui, Michihiko; Koide, Yoko; Yamamoto, Masahide

doi:10.7881/dentalmedres.29.28

Cited by 1 publication

(2 citation statements)

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“…Periodontal ligament cells containing both high and low ALP activities (ALP(+)PDL and ALP(−)PDL, respectively) coexist in periodontal ligament tissue. Of these, the cell proliferation of ALP(+)PDL is slower than that of ALP(−)PDL, but ALP(+)PDL appears as an osteoblast-like cell population [ 36 ]. We demonstrated that the proliferation of ALP(+)-HUCPVCs was significantly slower, but the mRNA levels of COL I and OPN were elevated, although RUNX2 gene expression showed no significant change.…”

Section: Discussionmentioning

confidence: 99%

“…Components of the extracellular matrix, such as biglycan and decorin, are listed as candidates for this regulator; they may regulate osteoblast-like differentiation and mineralization in periodontal ligament [ 49 , 50 , 51 ]. In particular, it was shown that periodontal ligament cells containing high ALP activity (ALP(+)PDL) present in the periodontal ligament are promoted and regulated into osteoblast-like cells by biglycan [ 36 ]. We demonstrated that the culture media containing CM-PPU7 promoted the formation of mineralized nodules of PPU7, but that with CM-HUCPVC dramatically suppressed mineral induction.…”

Section: Discussionmentioning

confidence: 99%

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Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells

Nonoyama

Karakida

Chiba-Ohkuma

et al. 2021

Cells

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Human umbilical cord perivascular cells (HUCPVCs), harvested from human umbilical cord perivascular tissue, show potential for future use as an alternative to mesenchymal stromal cells. Here, we present the results for the characterization of the properties alkaline phosphatase-positive HUCPVCs (ALP(+)-HUCPVCs). These ALP(+)-HUCPVCs were created from HUCPVCs in this study by culturing in the presence of activated vitamin D3, an inhibitor of bone morphogenetic protein signaling and transforming growth factor-beta1 (TGF-β1). The morphological characteristics, cell proliferation, gene expression, and mineralization-inducing ability of ALP(+)-HUCPVCs were investigated at the morphological, biological, and genetic levels. ALP(+)-HUCPVCs possess high ALP gene expression and activity in cells and a slow rate of cell growth. The morphology of ALP(+)-HUCPVCs is fibroblast-like, with an increase in actin filaments containing alpha-smooth muscle actin. In addition to ALP expression, the gene expression levels of type I collagen, osteopontin, elastin, fibrillin-1, and cluster of differentiation 90 are increased in ALP(+)-HUCPVCs. ALP(+)-HUCPVCs do not have the ability to induce mineralization nodules, which may be due to the restriction of phosphate uptake into matrix vesicles. Moreover, ALP(+)-HUCPVCs may produce anti-mineralization substances. We conclude that ALP(+)-HUCPVCs induced from HUCPVCs by a TGF-β1 stimulation possess myofibroblast-like properties that have little mineralization-inducing ability.

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Section: Discussionmentioning

confidence: 99%