Characterization of alpha-1-acid glycoprotein as a potential biomarker for breast cancer

Luo, Qiong; Yin, Jun

doi:10.1080/21655979.2022.2036303

Cited by 11 publications

(7 citation statements)

References 22 publications

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“…GO analysis results indicated that the 31 EMRGs were mainly enriched in glycoprotein metabolic process, regulation of lipid biosynthetic process, and glycosyltransferase activity, among others. Alpha-1-acid glycoprotein (AGP) is a key glycoprotein in the glycoprotein metabolic process, and researchers have found that AGP serves as a biomarker for BC and that AGP knockdown can inhibit the production of the inflammatory factors interleukin (IL)-1β, IL-8, and tumor necrosis factor-α in BC cells [ 34 ]. KEGG enrichment analysis further revealed that 31 EMRGs were also significantly associated with metabolic pathways, glycosaminoglycan biosynthesis, glycerophospholipid metabolism, Apelin signaling pathway, and several others.…”

Section: Discussionmentioning

confidence: 99%

Identification and validation of DHCR7 as a diagnostic biomarker involved in the proliferation and mitochondrial function of breast cancer

Wang,

Fan,

Liu

et al. 2024

Aging

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Background: Energy metabolism has a complex intersection with pathogenesis and development of breast cancer (BC). This allows for the possibility of identifying energy-metabolism-related genes (EMRGs) as novel prognostic biomarkers for BC. 7-dehydrocholesterol reductase (DHCR7) is a key enzyme of cholesterol biosynthesis involved in many cancers, and in this paper, we investigate the effects of DHCR7 on the proliferation and mitochondrial function of BC. Methods: EMRGs were identified from the Gene Expression Omnibus (GEO) and MSigDB databases using bioinformatics methods. Key EMRGs of BC were then identified and validated by functional enrichment analysis, interaction analysis, weighted gene co-expression network analysis (WGCNA), least absolute shrinkage and selection operator (LASSO) regression, Cox analysis, and immune infiltration. Western blot, qRT-PCR, immunohistochemistry (IHC), MTT assay, colony formation assay and flow cytometry assay were then used to analyze DHCR7 expression and its biological effects on BC cells. Results: We identified 31 EMRGs in BC. These 31 EMRGs and related transcription factors (TFs), miRNAs, and drugs were enriched in glycerophospholipid metabolism, glycoprotein metabolic process, breast cancer, and cell cycle. Crucially, DHCR7 was a key EMRG in BC identified and validated by WGCNA, LASSO regression and receiver operating characteristic (ROC) curve analysis. High DHCR7 expression was significantly associated with tumor immune infiltration level, pathological M, and poor prognosis in BC. In addition, DHCR7 knockdown inhibited cell proliferation, induced apoptosis and affected mitochondrial function in BC cells. Conclusions: DHCR7 was found to be a key EMRG up-regulated in BC cells. This study is the first to our knowledge to report that DHCR7 acts as an oncogene in BC, which might become a novel therapeutic target for BC patients.

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Section: Discussionmentioning

confidence: 99%

Identification and validation of DHCR7 as a diagnostic biomarker involved in the proliferation and mitochondrial function of breast cancer

Wang,

Fan,

Liu

et al. 2024

Aging

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“…Actually, ORM1, or alpha-1 acid glycoprotein (AGP), which acts an acute response protein with multiple modulating activities, constitutes 1% to 3% of plasma proteins in humans and is mainly synthesized in the liver, has become a famous biomarker in many tumors such as lymphoma, bladder cancer, breast cancer, gastric cancer, as well as epithelial ovarian cancer [ 37 , 38 , 39 , 40 , 41 ]. The role of ORM1 as a biomarker of cervical disease is reported for the first time in our study.…”

Section: Discussionmentioning

confidence: 99%

The Plasma DIA-Based Quantitative Proteomics Reveals the Pathogenic Pathways and New Biomarkers in Cervical Cancer and High Grade Squamous Intraepithelial Lesion

Han

Zhang

Sun

et al. 2022

JCM

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Objective: The process of normal cervix changing into high grade squamous intraepithelial lesion (HSIL) and invasive cervical cancer is long and the mechanisms are still not completely clear. This study aimed to reveal the protein profiles related to HSIL and cervical cancer and find the diagnostic and prognostic molecular changes. Methods: Data-independent acquisition (DIA) analysis was performed to identify 20 healthy female volunteers, 20 HSIL and 20 cervical patients in a cohort to screen differentially expressed proteins (DEPs) for the HSIL and cervical cancer. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used for functional annotation of DEPs; the protein–protein interaction (PPI) and weighted gene co-expression network analysis (WGCNA) were performed for detection of key molecular modules and hub proteins. They were validated using the Enzyme-Linked Immunosorbent Assay (ELISA). Results: A total of 243 DEPs were identified in the study groups. GO and KEGG analysis showed that DEPs were mainly enriched in the complement and coagulation pathway, cholesterol metabolism pathway, the IL-17 signaling pathway as well as the viral protein interaction with cytokine and cytokine receptor pathway. Subsequently, the WGCNA analysis showed that the green module was highly correlated with the cervical cancer stage. Additionally, six interesting core DEPs were verified by ELISA, APOF and ORM1, showing nearly the same expression pattern with DIA. The area under the curve (AUC) of 0.978 was obtained by using ORM1 combined with APOF to predict CK and HSIL+CC, and in the diagnosis of HSIL and CC, the AUC can reach to 0.982. The high expression of ORM1 is related to lymph node metastasis and the clinical stage of cervical cancer patients as well as the poor prognosis. Conclusion: DIA-ELSIA combined analysis screened and validated two previously unexplored but potentially useful biomarkers for early diagnosis of HSIL and cervical cancer, as well as possible new pathogenic pathways and therapeutic targets.

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“…Li F et al reported urinary ORM1 is significantly elevated in urine of bladder cancer patients, compared with controls and benign cases, it may be associated with the inflammatory activation in bladder cancer patients or associated with vascular endothelial cells, especially angiogenically activated blood vessels 31 . Luo Q et al reported serum ORM1 has been shown to be upregulated in the serum of breast cancer patients, ORM1 expression is associated with proinflammatory factors such as interleukin (IL)-1β, IL-8, and tumor necrosis factor-α 32 , 33 . ORM1 is predominantly synthesized in the liver, hepatic diseases may have more influence on its expression, ORM1 can be induced in liver injury and activate the liver cell cycle to achieve liver regeneration 34 .…”

Section: Discussionmentioning

confidence: 99%

Salivary orosomucoid 1 as a biomarker of hepatitis B associated hepatocellular carcinoma

Zheng

Liu

et al. 2022

Sci Rep

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Saliva is rich in proteins, DNA, RNA and microorganisms, and can be regarded as a biomarker library. In order to explore a noninvasive and simple means of early screening for liver cancer, proteomics was used to screen salivary markers of hepatitis B associated liver cancer. We used mass spectrometry coupled isobaric tags for relative and absolute quantitation (iTRAQ)-technology to identify differentially expressed proteins (DEPs). Western blot, immunohistochemistry and enzyme linked immunosorbent assay were used to detect marker expression of in tissues and saliva. Statistical analysis was used to analyze the diagnostic efficacy of the markers was analyzed through statistical analyses. By comparing the hepatocellular carcinoma (HCC) group with non-HCC groups, we screened out 152 salivary DEPs. We found orosomucoid 1(ORM1) had significantly higher expression in saliva of HCC patients compared with non-HCC groups (p < 0.001) and the expression of ORM1 in liver cancer tissues was significantly higher than that in adjacent normal tissues (p < 0.001). The combination of salivary ORM1 and alpha-fetoprotein (AFP) showed reasonable specificities and sensitivities for detecting HCC. In a word, salivary ORM1 as a new biomarker of hepatitis B associated hepatocellular carcinoma, combination of salivary ORM1 and AFP as an improved diagnostic tool for hepatocellular carcinoma.

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Characterization of alpha-1-acid glycoprotein as a potential biomarker for breast cancer

Cited by 11 publications

References 22 publications

Identification and validation of DHCR7 as a diagnostic biomarker involved in the proliferation and mitochondrial function of breast cancer

Identification and validation of DHCR7 as a diagnostic biomarker involved in the proliferation and mitochondrial function of breast cancer

The Plasma DIA-Based Quantitative Proteomics Reveals the Pathogenic Pathways and New Biomarkers in Cervical Cancer and High Grade Squamous Intraepithelial Lesion

Salivary orosomucoid 1 as a biomarker of hepatitis B associated hepatocellular carcinoma

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