Characterization of amygdalin-degrading <i>Lactobacillus</i>
 species

Menon, Rani; Munjal, Neera; Sturino, Joseph M.

doi:10.1111/jam.12704

Cited by 11 publications

(5 citation statements)

References 47 publications

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“…The biological activity varies depending on the glycoconjugation of the phytochemical ( 17 ). Stimulation or lack of inhibition of growth of lactobacilli on a few available glycosylated phytochemicals, here referred to as plant glycosides (PGs), has been reported ( 18 , 19 ), but the role of lactobacilli in the bioconversion of PG has not been clear to date.…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus acidophilus Metabolizes Dietary Plant Glucosides and Externalizes Their Bioactive Phytochemicals

Theilmann

Goh

Nielsen

et al. 2017

mBio

100

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Therapeutically active glycosylated phytochemicals are ubiquitous in the human diet. The human gut microbiota (HGM) modulates the bioactivities of these compounds, which consequently affect host physiology and microbiota composition. Despite a significant impact on human health, the key players and the underpinning mechanisms of this interplay remain uncharacterized. Here, we demonstrate the growth of Lactobacillus acidophilus on mono- and diglucosyl dietary plant glycosides (PGs) possessing small aromatic aglycones. Transcriptional analysis revealed the upregulation of host interaction genes and identified two loci that encode phosphotransferase system (PTS) transporters and phospho-β-glucosidases, which mediate the uptake and deglucosylation of these compounds, respectively. Inactivating these transport and hydrolysis genes abolished or severely reduced growth on PG, establishing the specificity of the loci to distinct groups of PGs. Following intracellular deglucosylation, the aglycones of PGs are externalized, rendering them available for absorption by the host or for further modification by other microbiota taxa. The PG utilization loci are conserved in L. acidophilus and closely related lactobacilli, in correlation with versatile growth on these compounds. Growth on the tested PG appeared more common among human gut lactobacilli than among counterparts from other ecologic niches. The PGs that supported the growth of L. acidophilus were utilized poorly or not at all by other common HGM strains, underscoring the metabolic specialization of L. acidophilus. These findings highlight the role of human gut L. acidophilus and select lactobacilli in the bioconversion of glycoconjugated phytochemicals, which is likely to have an important impact on the HGM and human host.

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Section: Introductionmentioning

confidence: 99%

Lactobacillus acidophilus Metabolizes Dietary Plant Glucosides and Externalizes Their Bioactive Phytochemicals

Theilmann

Goh

Nielsen

et al. 2017

mBio

100

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“…Degradation of amygdalin by microorganisms has widely been documented in mammal micro ora 24 , Lactobacillus paraplantarum and Lactobacillus plantarum 25 . Likewise, the larva-originated bacteria cultured using minimum medium with amygdalin as a unique carbon source (MM) could e ciently degrade the cyanoglucoside, accompanied by an increase in prunasin.…”

Section: Bacterial Symbionts Produce Cyano-compounds In Their Host Be...mentioning

confidence: 99%

Proteus vulgaris strain Ld01-originated mandelonitrile defends Leptinotarsa decemlineata larvae and adults against predation

Li,

Kang,

Zhang

et al. 2023

Preprint

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Leptinotarsa decemlineata larvae and adults have evolved to apply aposematic signals to display their toxicity to natural enemies. Nevertheless, the chemical components of the volatile repellant remain to be identified. Here we show that the larvae and adults actively release hypertoxic hydrogen cyanide (HCN) from the degradation of nitriles, in contrast to the pupae. A bacterial symbiont Proteus vulgaris Ld01 is responsible for the production of a cyanoglucoside, amygdalin. The bacterium mainly lives in beetle guts and proliferates rapidly during ecdysis. Removal of larval bacteria significantly reduces HCN emission in the larvae and adults. Re-infection with the symbiont in or addition of mandelonitrile to the bacteria-deprived beetles restores the emission. Deprivation of bacteria decreases the defense of the larvae and adults against chick predation, which can be mitigated by re-colonization of P. vulgaris Ld01 or introduction of mandelonitrile. Together, these findings highlight the roles of intestinal P. vulgaris Ld01 in the production of an aromatic cyanoglucoside amygdalin in its host L. decemlineata. Degradation of amygdalin liberates an aposematic compound mandelonitrile to the hemolymph. Mandelonitrile and its metabolites are accumulated near the cuticle, where they are converted to HCN to defend the beetles against predation.

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“…Apple seeds can contain up to 4 mg/g of this glucoside, and that is why commercial apple juice usually contains 0.1 mg/mL of amygdalin [ 326 ]. LAB converting cyanogenic glycosides are relatively rare: among 25 strains representing 23 species of LAB screened by Menon et al [ 327 ], only Lp. plantarum and Lp.…”

Section: Lab In Detoxication Of Food From Natural Antinutrientsmentioning

confidence: 99%

The Complex Role of Lactic Acid Bacteria in Food Detoxification

et al. 2022

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Toxic ingredients in food can lead to serious food-related diseases. Such compounds are bacterial toxins (Shiga-toxin, listeriolysin, Botulinum toxin), mycotoxins (aflatoxin, ochratoxin, zearalenone, fumonisin), pesticides of different classes (organochlorine, organophosphate, synthetic pyrethroids), heavy metals, and natural antinutrients such as phytates, oxalates, and cyanide-generating glycosides. The generally regarded safe (GRAS) status and long history of lactic acid bacteria (LAB) as essential ingredients of fermented foods and probiotics make them a major biological tool against a great variety of food-related toxins. This state-of-the-art review aims to summarize and discuss the data revealing the involvement of LAB in the detoxification of foods from hazardous agents of microbial and chemical nature. It is focused on the specific properties that allow LAB to counteract toxins and destroy them, as well as on the mechanisms of microbial antagonism toward toxigenic producers. Toxins of microbial origin are either adsorbed or degraded, toxic chemicals are hydrolyzed and then used as a carbon source, while heavy metals are bound and accumulated. Based on these comprehensive data, the prospects for developing new combinations of probiotic starters for food detoxification are considered.

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Characterization of amygdalin-degrading Lactobacillus species

Abstract: Amygdalin-degrading lactobacilli with practical application in the biodetoxification of amygdalin were characterized. These strains show potential for use as starter cultures to improve the safety of foods and feeds.

Cited by 11 publications

References 47 publications

Lactobacillus acidophilus Metabolizes Dietary Plant Glucosides and Externalizes Their Bioactive Phytochemicals

Lactobacillus acidophilus Metabolizes Dietary Plant Glucosides and Externalizes Their Bioactive Phytochemicals

Proteus vulgaris strain Ld01-originated mandelonitrile defends Leptinotarsa decemlineata larvae and adults against predation

The Complex Role of Lactic Acid Bacteria in Food Detoxification

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