Characterization of an adulticidal and larvicidal interfering RNA pesticide that targets a conserved sequence in mosquito G protein-coupled dopamine 1 receptor genes

Hapairai, Limb K.; Mysore, Keshava; Sun, Lili; Li, Ping; Wang, Chien Wei; Scheel, Nicholas D.; Lesnik, Alexandra; Scheel, Max P.; Igiede, Jessica; Wei, Na; Severson, David W.; Duman-Scheel, Molly

doi:10.1016/j.ibmb.2020.103359

Cited by 25 publications

(41 citation statements)

References 62 publications

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“…During EPA deliberations, it was noted that organisms routinely consume interfering RNA molecules, which are endogenously produced in many plant and animal species, suggesting that consumption of interfering RNA that lacks specific target sites in non-target species would pose little if any risk to these organisms [ 31 ]. Our own initial toxicity assays of several yeast interfering RNA larvicides in non-target organisms [ 19 – 21 ] support this concept. In the EPA deliberations [ 31 ], it was also noted that in humans, orally consumed interfering RNA is not expected to last in the gastrointestinal tract.…”

Section: Discussionmentioning

confidence: 92%

“…It is therefore hypothesized that interfering RNA could be delivered in dried yeast prepared in bulk and distributed in a ready-to-use tablet formulations, and that this technology would be broadly accepted by consumers [ 18 ]. In support of this, recent laboratory and semi-field investigations have confirmed the efficacy of dried yeast larvicidal tablets [ 19 – 21 ], and the potential for scaled production of commercial-ready formulations is presently being investigated [ 18 ]. This investigation evaluated potential stakeholder acceptance of this new intervention in Trinidad.…”

Section: Introductionmentioning

confidence: 99%

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Community acceptance of yeast interfering RNA larvicide technology for control of Aedes mosquitoes in Trinidad

et al. 2020

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RNA interference (RNAi), a technique used to investigate gene function in insects and other organisms, is attracting attention as a potential new technology for mosquito control. Saccharomyces cerevisiae (baker's yeast) was recently engineered to produce interfering RNA molecules that silence genes required for mosquito survival, but which do not correspond to genes in humans or other non-target organisms. The resulting yeast pesticides, which facilitate cost-effective production and delivery of interfering RNA to mosquito larvae that eat the yeast, effectively kill mosquitoes in laboratory and semi-field trials. In preparation for field evaluation of larvicides in Trinidad, a Caribbean island with endemic diseases resulting from pathogens transmitted by Aedes mosquitoes, adult residents living in the prospective trial site communities of Curepe, St. Augustine, and Tamana were engaged. Open community forums and paper surveys were used to assess the potential acceptability, societal desirability, and sustainability of yeast interfering RNA larvicides. These assessments revealed that Trinidadians have good working knowledge of mosquitoes and mosquito-borne illnesses. A majority of the respondents practiced some method of larval mosquito control and agreed that they would use a new larvicide if it were proven to be safe and effective. During the community engagement forums, participants were educated about mosquito biology, mosquitoborne diseases, and the new yeast larvicides. When invited to provide feedback, engagement forum attendees were strongly supportive of the new technology, raised few concerns, and provided helpful advice regarding optimal larvicide formulations, insecticide application, operational approaches for using the larvicides, and pricing. The results of these studies suggest that the participants are supportive of the potential use of yeast interfering RNA larvicides in Trinidad and that the communities assessed in this investigation represent viable field sites.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Community acceptance of yeast interfering RNA larvicide technology for control of Aedes mosquitoes in Trinidad

et al. 2020

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“…quinquefasciatus [ 141 ], while an RNAi technique that targets the dopamine 1 receptor also led to the death of both larvae and adult in Ae. aegypti [ 142 ]. Many more chemicals could be investigated to target various GPCR receptors and improve insect management ( Table 3 ).…”

Section: Whole Genome Sequencing and Transcriptome Analysis—sequence Comparison And Gpcr Characterization In Insectsmentioning

confidence: 99%

G-Protein Coupled Receptors (GPCRs) in Insects—A Potential Target for New Insecticide Development

Liu

Wang

et al. 2021

Molecules

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G-protein coupled receptors (GPCRs) play important roles in cell biology and insects’ physiological processes, toxicological response and the development of insecticide resistance. New information on genome sequences, proteomic and transcriptome analysis and expression patterns of GPCRs in organs such as the central nervous system in different organisms has shown the importance of these signaling regulatory GPCRs and their impact on vital cell functions. Our growing understanding of the role played by GPCRs at the cellular, genome, transcriptome and tissue levels is now being utilized to develop new targets that will sidestep many of the problems currently hindering human disease control and insect pest management. This article reviews recent work on the expression and function of GPCRs in insects, focusing on the molecular complexes governing the insect physiology and development of insecticide resistance and examining the genome information for GPCRs in two medically important insects, mosquitoes and house flies, and their orthologs in the model insect species Drosophila melanogaster. The tissue specific distribution and expression of the insect GPCRs is discussed, along with fresh insights into practical aspects of insect physiology and toxicology that could be fundamental for efforts to develop new, more effective, strategies for pest control and resistance management.

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“…The main function of GPCRs is to transduce extracellular signals and regulate intracellular second messengers through coupling to heterotrimeric G-proteins and their downstream effectors. As a result of the critical functions of GPCRs in cell physiology and biochemistry, they are important targets for the development of clinical medicines for a wide range of human disease therapies [ 1 , 2 , 3 , 4 ] and new and potentially more effective chemicals for insect pest management [ 5 , 6 , 7 ]. GPCRs are involved in recognizing extracellular messengers, transducing signals to the cytosol, and mediating the cellular responses necessary for the normal physiological functions of organisms [ 8 , 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

G-Protein Coupled Receptors (GPCRs): Signaling Pathways, Characterization, and Functions in Insect Physiology and Toxicology

Liu

Wang

et al. 2021

IJMS

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G-protein-coupled receptors (GPCRs) are known to play central roles in the physiology of many organisms. Members of this seven α-helical transmembrane protein family transduce the extracellular signals and regulate intracellular second messengers through coupling to heterotrimeric G-proteins, adenylate cyclase, cAMPs, and protein kinases. As a result of the critical function of GPCRs in cell physiology and biochemistry, they not only play important roles in cell biology and the medicines used to treat a wide range of human diseases but also in insects’ physiological functions. Recent studies have revealed the expression and function of GPCRs in insecticide resistance, improving our understanding of the molecular complexes governing the development of insecticide resistance. This article focuses on the review of G-protein coupled receptor (GPCR) signaling pathways in insect physiology, including insects’ reproduction, growth and development, stress responses, feeding, behaviors, and other physiological processes. Hormones and polypeptides that are involved in insect GPCR regulatory pathways are reviewed. The review also gives a brief introduction of GPCR pathways in organisms in general. At the end of the review, it provides the recent studies on the function of GPCRs in the development of insecticide resistance, focusing in particular on our current knowledge of the expression and function of GPCRs and their downstream regulation pathways and their roles in insecticide resistance and the regulation of resistance P450 gene expression. The latest insights into the exciting technological advances and new techniques for gene expression and functional characterization of the GPCRs in insects are provided.

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Characterization of an adulticidal and larvicidal interfering RNA pesticide that targets a conserved sequence in mosquito G protein-coupled dopamine 1 receptor genes

Cited by 25 publications

References 62 publications

Community acceptance of yeast interfering RNA larvicide technology for control of Aedes mosquitoes in Trinidad

Community acceptance of yeast interfering RNA larvicide technology for control of Aedes mosquitoes in Trinidad

G-Protein Coupled Receptors (GPCRs) in Insects—A Potential Target for New Insecticide Development

G-Protein Coupled Receptors (GPCRs): Signaling Pathways, Characterization, and Functions in Insect Physiology and Toxicology

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