Characterization of an algal extract by HPLC-ICP-MS and LC-electrospray MS for use in arsenosugar speciation studies

Madsen, Anne D.; Goessler, Walter; Pedersen, Søren N.; Francesconi, Kevin A.

doi:10.1039/b001418o

Cited by 193 publications

(145 citation statements)

References 19 publications

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…Concentrations of AB in L. rubellus and D. rubidus from uncontaminated soils of 0.3 and 0.5 mg kg -1 respectively have previously been reported, 2 falling within the range reported for contaminated sites. This reinforces the point that AB formation in earthworms is 10 independent of total As burdens and not involved in mitigating As toxicity. The resistance is more likely due to a second process (Fig.…”

Section: Discussionsupporting

confidence: 81%

“…The method precision, 10 expressed as the mean % difference (± 1 SD), between duplicate earthworm samples was 1.7 ± 0.9 % (n= 4 duplicates). The extraction procedure employed gave a mean recovery of 77 ± 1 % (n = 3) of the total arsenic value for CRM 627 with a precision, expressed as the mean percentage 15 difference (± 1 SD) between duplicate samples, of 2.8 ± 1.8 % (n = 4 duplicates).…”

Section: Earthwormsmentioning

confidence: 99%

“…23 In brief, 0.2 g of each prepared sample was accurately weighed into 30 ml round-bottom Nalgene® extraction vessels, 10 ml of a 1 M phosphoric acid / 0.5 M ascorbic acid mixture was then added and the lids securely fastened. The extraction vessels were 10 then attached to an orbital shaker and extracted for 4 hours at 200 rpm. Extractions were conducted in the dark to avoid speciation changes due to UV radiation.…”

Section: Sample Extraction Soils and Castsmentioning

confidence: 99%

See 2 more Smart Citations

Arsenic biotransformation in earthworms from contaminated soils

et al. 2009

View full text Add to dashboard Cite

Two species of arsenic (As) resistant earthworm, Lumbricus rubellus and Dendrodrillus rubidus, their host soils and soil excretions (casts) were collected from 23 locations at a former As mine in 5 Devon, UK. Total As concentrations, measured by ICP-MS, ranged from 255 to 13,080 mg kg -1 in soils, 11 to 877 mg kg -1 in earthworms and 284 to 4221 mg kg -1 in earthworm casts from a subsample of 10 of the 23 investigated sites. The samples were also measured for As speciation using HPLC-ICP-MS to investigate potential As biotransformation pathways. Inorganic arsenate (As V ) and arsenite (As III ) were the only species detected in the soil. As V and As III were also the dominant 10 species found in the earthworms and cast material together with lower proportions of the organic species methylarsonate (MA V ), dimethylarsinate (DMA V ), arsenobetaine (AB) and three arsenosugars. Whilst the inorganic As content of the earthworms increased with increasing As body burden, the concentration of organic species remained relatively constant. These results suggest that the biotransformation of inorganic arsenic to organic species does not contribute to As 15 resistance in the sampled earthworm populations. Quantification of As speciation in the soil, earthworms and cast material allows a more comprehensive pathway for the formation of AB in earthworms to be elucidated. Received (in XXX, IntroductionThe chemistry of As in environmental and biological systems is complex. Whilst inorganic As species are the most prevalent in abiotic environments, the uptake of inorganic As 25 by living organisms can lead to the synthesis of organic As species 1 through biotransformation. The ubiquitous, epegeic earthworm species L. rubellus and D. rubidus are known to inhabit soils highly contaminated with As at the former mine site of Devon Great Consols (DGC), UK. [2][3][4][5] These earthworms 30 are clearly resistant to As toxicity and several potential coping mechanisms have been proposed, yet the underlying mechanism behind this resistance is unknown. Behavioural adaptation, whereby the earthworm avoids contact with the contaminant, 4 is unlikely as earthworms from DGC are known 35 to have elevated As body burdens. 2,4, 5 The biotransformation of highly toxic inorganic As to the less toxic organic species arsenobetaine (AB) has been speculated as a mode of mitigating As toxicity in DGC earthworms. 2,4 An alternative mechanism involves the sequestration of arsenic in the 40 metallothionein-rich chloragogenous tissue which separates the intestine from the coelomic cavity. 3 With this mechanism it is proposed that inorganic As III binds to the sulphur-rich metallothionein thereby sequestering ingested As in a form that is not biologically reactive. The study of As speciation can provide important information on As biotransformation and toxicity and to date a multitude of organic As species have been identified. 7 The occurrence of organic As species and their biotransformation pathways are well documented in marine organisms such as crus...

show abstract

Section: Discussionsupporting

confidence: 81%

Section: Earthwormsmentioning

confidence: 99%

Section: Sample Extraction Soils and Castsmentioning

confidence: 99%

See 1 more Smart Citation

Arsenic biotransformation in earthworms from contaminated soils

et al. 2009

View full text Add to dashboard Cite

show abstract

“…We did not obtain promising results by a brief survey of silica-gel-based ion-exchange columns-organic buffer combination, possibly because of unexpected interaction of phenyl groups with either hydrophobic linker or end-capping materials. Polymer-based anion-exchange columns based on styrene-divinylbenzene co-polymer, which have been used frequently for speciation of arsenic, 18 were not checked because of expected strong hydrophobic interaction between benzene rings. In our previously established aqueous ion-pair condition for the separation of organic arsenic compounds in marine life (LC-1 14 ), DPAA could not be eluted at all from a C18 column.…”

Section: Development Of Hplc-icp-ms Condition For Dpaa Analysismentioning

confidence: 99%

Analysis of diphenylarsinic acid in human and environmental samples by HPLC-ICP-MS

Shibata

Tsuzuku

Komori

et al. 2005

Appl. Organometal. Chem.

View full text Add to dashboard Cite

A simple, rapid and robust analytical method for determining diphenylarsinic acid in human and environmental samples was developed based on a combination of hydrophilic polymer-based gelpermeation high-performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS). Hair and nail samples were digested with alkali, and liberated diphenylarsinic acid (derivative) was extracted with diethyl ether, redissolved in water and injected for HPLC-ICP-MS analysis. Human urine, groundwater and water extracts from soils were injected for HPLC-ICP-MS directly after filtration. Using the method, diphenylarsinic acid in a solution was quantified in 7 min duration for an analysis with a detection limit of sub-nanograms per milliliter. The method has been applied to groundwater arsenic pollution recently uncovered in Japan.

show abstract

“…MA was used as an appropriate calibrant for these three arsenosugars, since it eluted within the same eluent concentration of 4 mM NH 3 NO 3 . Madsen et al (2000) also used MA as a calibrant for the quantification of arsenosugars. Calibration for arsenic speciation on the sediments was performed by standard addition of the arsenic species to the NRCC BCSS-1 Marine sediment.…”

Section: Arsenic Speciationmentioning

confidence: 99%

Arsenic speciation in polychaetes (Annelida) and sediments from the intertidal mudflat of Sundarban mangrove wetland, India

Watts

Barlow

Button

et al. 2012

Environ Geochem Health

View full text Add to dashboard Cite

Abstract:This paper documents the concentration of total arsenic and individual arsenic species in four soft-bottom benthic polychaetes (Perenereis cultifera, Ganganereis sootai, Lumbrinereis notocirrata and Dendronereis arborifera) along with host sediments from Sundarban mangrove wetland, India. An additional six sites were considered exclusively for surface sediments for this purpose. Polychaetes were collected along with the host sediments and measured for their total arsenic using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Arsenic concentrations in polychaete body tissues varied greatly, suggesting speciesspecific characteristics and inherent peculiarities in arsenic metabolism. Arsenic was generally present in polychaetes as arsenate (As V ranges from 0.16 -0.50 mg kg -1 ) or arsenite (As III ranges from 0.10 -0.41 mg kg -1 ) (30 to 53% as inorganic As) and dimethylarsinic acid (DMA V ; <1 to 25%). Arsenobetaine (AB; <16%), and PO 4 -arsenoriboside (8 to 48%) were also detected as minor constituents, whilst monomethylarsonic acid (MA V ) was not detected in any of the polychaetes. The highest total As (14.7 mg kg -1 dry wt) was observed in the polychaete D. arborifera collected from the vicinity of a sewage outfall in which the majority of As was present as an uncharacterized compound (10.3 mg kg -1 dry wt) eluted prior to AB. Host sediments ranged from 2.5 to 10.4 mg kg -1 total As. This work supports the importance of speciation analysis of As, because of the ubiquitous occurrence of this metalloid in the environment, and its variable toxicity depending on chemical form. It is also the first work to report the composition of As species in polychaetes from the Indian Sundarban wetlands.

show abstract

Characterization of an algal extract by HPLC-ICP-MS and LC-electrospray MS for use in arsenosugar speciation studies

Cited by 193 publications

References 19 publications

Arsenic biotransformation in earthworms from contaminated soils

Arsenic biotransformation in earthworms from contaminated soils

Analysis of diphenylarsinic acid in human and environmental samples by HPLC-ICP-MS

Arsenic speciation in polychaetes (Annelida) and sediments from the intertidal mudflat of Sundarban mangrove wetland, India

Contact Info

Product

Resources

About