Characterization of an Antibacterial Compound, 2-Hydroxyl Indole-3-Propanamide, Produced by Lactic Acid Bacteria Isolated from Fermented Batter

Jeevaratnam, Kadirvelu; Vidhyasagar, Venkatasubramanian; Agaliya, Perumal Jayaprabha; Saraniya, Appukuttan; Umaiyaparvathy, Muthukandan

doi:10.1007/s12010-015-1733-9

Cited by 17 publications

(7 citation statements)

References 28 publications

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“…The inhibitory spectrum of compounds in the fractions AI and AII produced by L. lactis ID1.5 is presented in 4). Recently, low molecular weight compound produced by Lactobacillus and Pediococcus has been described as antimicrobial against Gram-positive and Gram-negative bacteria (Jeevaratnam et al 2015). Interestingly, in our work the compound AI showed antimicrobial activity against all L. lactis strains tested (Table 4).…”

Section: Determination Of Inhibitory Spectrumsupporting

confidence: 55%

Purification and characterization of two new cell-bound bioactive compounds produced by wild Lactococcus lactis strain

Saraiva

Brede

Nes

et al. 2017

FEMS Microbiology Letters

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Novel compounds and innovative methods are required considering that antibiotic resistance has reached a crisis point. In the study, two cell-bound antimicrobial compounds produced by Lactococcus lactis ID1.5 were isolated and partially characterized. Following purification by cationic exchange and a solid-phase C18 column, antimicrobial activity was recovered after three runs of RPC using 60% (v/v) and 100% (v/v) of 2-propanol for elution, suggesting that more than one antimicrobial compound were produced by L. lactis ID1.5, which were in this study called compounds AI and AII. The mass spectrum of AI and AII showed major intensity ions at m/z 1070.05 and 955.9 Da, respectively. The compound AI showed a spectrum of antimicrobial activity mainly against L. lactis species, while the organisms most sensitive to compound AII were Bacillus subtilis, Listeria innocua, Streptococcus pneumoniae and Pseudomonas aeruginosa. The antimicrobial activity of both compounds was suppressed by treatment with Tween 80. Nevertheless, both compounds showed high stability to heat and proteases treatments. The isolated compounds, AI and AII, showed distinct properties from other antimicrobial substances already reported as produced by L. lactis, and have a significant inhibitory effect against two clinically important respiratory pathogens.

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Section: Determination Of Inhibitory Spectrumsupporting

confidence: 55%

Purification and characterization of two new cell-bound bioactive compounds produced by wild Lactococcus lactis strain

Saraiva

Brede

Nes

et al. 2017

FEMS Microbiology Letters

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“…isolated from dosa batter was beneficial in their selection for future food and feed applications [15]. The chemical compound (2-hydroxyl indole-3-propanamide) derived from LAB strains isolated from idly and uttapam batter has potential antibiotic properties that protect probiotic bacteria against the pathogenic strains [16]. With this background, the study objectives are to isolate LAB from dosa batter that has probiotic potential and study its inhibitory potential against the carbohydrate hydrolyzing enzymes (α-glucosidase and α-amylase).…”

Section: Introductionmentioning

confidence: 99%

Probiotic Potential Lacticaseibacillus casei and Limosilactobacillus fermentum Strains Isolated from Dosa Batter Inhibit α-Glucosidase and α-Amylase Enzymes

Huligere

Shbeer

et al. 2022

Microorganisms

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Fermented food plays a major role in gastrointestinal health, as well as possesses other health benefits, such as beneficiary effects in the management of diabetes. Probiotics are thought to be viable sources for enhancing the microbiome of the human gut. In the present study, using biochemical, physiological, and molecular approaches, the isolated Lactobacillus spp. from dosa batter were identified. The cell-free supernatant (CS), cell-free extract (CE), and intact cells (IC) were evaluated for their inhibitory potential against the carbohydrate hydrolyzing enzymes α-glucosidase and α-amylase. Then, 16S rDNA amplification and sequencing were used to identify the species. A homology search in NCBI database was performed that suggests the isolates are >95% similar to Limosilactobacillus fermentum and Lacticaseibacillus casei. Different standard parameters were used to evaluate the probiotic potential of strains RAMULAB07, RAMULAB08, RAMULAB09, RAMULAB10, RAMULAB11, and RAMULAB12. The strains expressed a significant tolerance to the gastric and intestinal juices with a higher survival rate (>98%). A high adhesion capability was observed by the isolates exhibited through hydrophobicity (>65%), aggregation assays (>75%), and adherence assay on HT-29 cells (>82%) and buccal epithelial cells. In addition, the isolates expressed antibacterial and antibiotic properties. Safety assessments (DNase and hemolytic assay) revealed that the isolates could be classified as safe. α-glucosidase and α-amylase inhibition of the isolates for CS, CE, and IC ranged from 7.50% to 65.01% and 20.21% to 56.91%, respectively. The results suggest that these species have exceptional antidiabetic potential, which may be explained by their use as foods that can have health-enhancing effects beyond basic nutrition.

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“…Another novel LMW compound was obtained from Lactiplantibacillus plantarum KJB23 studied by our group which was found to be resistant to acid protease (Kavitha et al 2020). Previous studies reported other antimicrobial LMW compounds, such as phenyl lactic acids and 2-Hydroxyl Indole-3-Propanamide, produced by Lactiplantibacillus plantarum (Yang et al 1997;Schwenninger et al 2008;Jeevaratnam et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Targeting Staphylococcus aureus and its biofilms with novel antibacterial compounds produced by Lactiplantibacillus plantarum SJ33

et al. 2021

Self Cite

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The emergence of microbial resistance to conventional antibiotics, partially attributed to biofilm formation, has urged for new antimicrobial compounds. Here, we reported two novel low molecular weight (LMW) compounds from Lactiplantibacillus plantarum SJ33 and evaluated their biofilm inhibitory effects on Staphylococcus aureus. The compounds C1 and C2 were purified by RP-HPLC and structurally identified as 3-amino-5-hydroxy-6-(hydroxymethyl)-4-(1-hydroxyprop-2yn-1-yl)-3,3a,4,5,6,7a-hexahydro-7H-indazol-7-one and 1-(dimethylamino)-3-hydroxy-3-((2-hydroxypropan-2-yl)oxy)-1-(methylamino)-butan-2-one by spectroscopic techniques. High ESI-MS data confirmed the molecular weight of C1 and C2 as 254.1141 and 234.1658 Da, respectively. Time-kill assay demonstrated bactericidal action of compounds, whereas scanning electron microscopy revealed morphological changes in treated S. aureus MTCC96 and methicillin-resistant S. aureus (MRSA) cells. The antibacterial compounds reduced biofilm formation in S. aureus MTCC96 and MRSA by crystal violet assay. Further, fluorescence and scanning electron microscopic images exhibited biofilm formation by pathogens and biofilm inhibition by compounds treatment. The Quantitative RT PCR revealed the down-regulation of icaC and icaD genes involved in intercellular adhesion of biofilms. The results confirmed the anti-biofilm activity of novel LMW compounds by eliminating preformed biofilms formed by S. aureus MTCC96 and MRSA.

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Characterization of an Antibacterial Compound, 2-Hydroxyl Indole-3-Propanamide, Produced by Lactic Acid Bacteria Isolated from Fermented Batter

Cited by 17 publications

References 28 publications

Purification and characterization of two new cell-bound bioactive compounds produced by wild Lactococcus lactis strain

Purification and characterization of two new cell-bound bioactive compounds produced by wild Lactococcus lactis strain

Probiotic Potential Lacticaseibacillus casei and Limosilactobacillus fermentum Strains Isolated from Dosa Batter Inhibit α-Glucosidase and α-Amylase Enzymes

Targeting Staphylococcus aureus and its biofilms with novel antibacterial compounds produced by Lactiplantibacillus plantarum SJ33

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