Characterization of an antimicrobial dental resin adhesive containing zinc methacrylate

Henn, Sandrina; Nedel, Fernanda; Carvalho, Rodrigo Varella de; Lund, Rafael Guerra; Cenci, Maximiliano Sérgio; Pereira‐Cenci, Tatiana; Demarco, Flávio Fernando; Piva, Evandro

doi:10.1007/s10856-011-4364-x

Cited by 29 publications

(28 citation statements)

References 24 publications

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“…The viability of the HT-29 cells was determined by measuring the reduction of soluble MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] to water insoluble formazan (Ali et al, 2010;Henn et al, 2011). Briefly, cells were seeded at a density of 2 × 10 4 cells per well in a volume of 100 μL in 96-well plates and grown at 37°C in a humidified atmosphere of 5% CO 2 /95% air for 24 h before being used in the MTT assay.…”

Section: Determination Of Cytotoxicitymentioning

confidence: 99%

Substituted diaryl diselenides: Cytotoxic and apoptotic effect in human colon adenocarcinoma cells

et al. 2012

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Section: Determination Of Cytotoxicitymentioning

confidence: 99%

Substituted diaryl diselenides: Cytotoxic and apoptotic effect in human colon adenocarcinoma cells

et al. 2012

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“…Examples of antibacterial components added to contemporary adhesive systems include glutaraldehyde (Felton et al 1989;Schmidlin et al 2004), 12-methacryloyloxy dodecylpyridinium bromide (MDPB), and most recently, methacrylate dimethyl ammonium chloride (Imazato et al 1998;Xiao et al 2009;Henn et al 2011). Given the incidence of dental caries, increased resistance by bacteria to antibiotics, the adverse effects of some antibacterial agents currently used in dentistry and the financial considerations in developing countries, there is a need for alternative prevention and treatment options that are safe, effective, and economical.…”

Section: Introductionmentioning

confidence: 99%

Self-etching dental adhesive containing a natural essential oil: anti-biofouling performance and mechanical properties

Peralta¹,

Carvalho²,

Sande³

et al. 2013

Biofouling

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This study assessed the anti-biofouling performance of an experimental adhesive system containing a naturally occurring essential vegetable oil and examined the following physical and mechanical properties: water sorption (WS) and solubility (SL), microtensile bond strength to dentin (μTBS), and degree of conversion. The following six groups were tested: a self-etching experimental adhesive containing refined essential oil from the seeds of the Butia capitata tree (EA O ); an oil-free version of the experimental adhesive (EA NO ); one group without adhesive as the control (C); and the three following commercial self-etching adhesives: Clearfil Protect Bond (CPB), Clearfil SE Bond, and Adper SE Plus. The antibacterial effect was estimated by microbiological culture on selective/non-selective media, and the results expressed as colony-forming units per unit weight of dry biofilm (CFU mg À1). The data were submitted to ANOVA and Tukey's post hoc test (α = 0.05). After 24 h, pH changes were similar in the storage medium of all tested adhesive systems. EA O showed similar levels of antimicrobial activity in a model biofilm microcosm as the commercial self-etching adhesive CPB. Both were effective against total microorganisms, aciduric bacteria, lactobacilli, and Streptococcus mutans. WS and SL were not affected by the presence of the essential oil; the values of EA O were similar to or less than those of commercial equivalents. The incorporation of an essential oil into an experimental adhesive did not influence its monomer conversion result. Immediate μTBS values of EA O and EA NO were similar and were greater than those of commercial equivalents. After storage for 6 months, the μTBS of the EA O decreased significantly and became similar to the values of commercial equivalents, while the strength of the EA NO was not affected.

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“…Mouse fibroblasts were maintained as a stock culture in DMEM and incubated at 37°C in a humidified atmosphere of 5% CO 2 in air until subconfluency was reached, as described previously [26, 27]. AH alone and combined with nfTD and nfHY was sterilized by exposure to germicidal UV (ultraviolet) light for 40 min.…”

Section: Methodsmentioning

confidence: 99%

Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering

Santana

Nedel

Piva

et al. 2013

BioMed Research International

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We aimed to develop an alginate hydrogel (AH) modified with nano-/microfibers of titanium dioxide (nfTD) and hydroxyapatite (nfHY) and evaluated its biological and chemical properties. Nano-/microfibers of nfTD and nfHY were combined with AH, and its chemical properties were evaluated by FTIR spectroscopy, X-ray diffraction, energy dispersive X-Ray analysis, and the cytocompatibility by the WST-1 assay. The results demonstrate that the association of nfTD and nfHY nano-/microfibers to AH did not modified the chemical characteristics of the scaffold and that the association was not cytotoxic. In the first 3 h of culture with NIH/3T3 cells nfHY AH scaffolds showed a slight increase in cell viability when compared to AH alone or associated with nfTD. However, an increase in cell viability was observed in 24 h when nfTD was associated with AH scaffold. In conclusion our study demonstrates that the combination of nfHY and nfTD nano-/microfibers in AH scaffold maintains the chemical characteristics of alginate and that this association is cytocompatible. Additionally the combination of nfHY with AH favored cell viability in a short term, and the addition of nfTD increased cell viability in a long term.

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Characterization of an antimicrobial dental resin adhesive containing zinc methacrylate

Cited by 29 publications

References 24 publications

Substituted diaryl diselenides: Cytotoxic and apoptotic effect in human colon adenocarcinoma cells

Substituted diaryl diselenides: Cytotoxic and apoptotic effect in human colon adenocarcinoma cells

Self-etching dental adhesive containing a natural essential oil: anti-biofouling performance and mechanical properties

Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering

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