Characterization of an Antimicrobial Extract from Elaeagnus angustifolia

Abstract

Background: According to ethnobotanical data, Elaeagnus angustifolia fruit has wound healing activity, anti-inflammatory effect and antifebrile prosperities. Objectives: This study was performed as to the best of our knowledge; there has been no scientific report on the characterization of antimicrobial effect of E. angustifolia extract. Materials and Methods: An aqueous extract of Elaeagnus angustifolia was prepared and antimicrobial activity tests were performed on various target cultures. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the extract was done using the broth dilution technique. To characterize the extract, shelf life, thermal and pH stability, effects of detergents such as Tween 80, Tween 20, Triton X100, toluene and enzymes on the antimicrobial activity of Elaeagnus angustifolia extract, were examined. Results:The MIC values ranged from 7.5 to 0.1 mg/mL, showing maximum activity (1.62 mg/mL) against E. coli. Similarly, the MBC of the extract against E. coli was 1.62 mg/mL. Antimicrobial activity of the extract was relatively stable when kept in the refrigerator for 60 days. The antimicrobial activity of Elaeagnus angustifolia extract was absolutely stable at temperatures up to 700° C. After exposure of the Elaeagnus angustifolia extract to different pH solutions in the range of 4-10, almost 100% residual activity was found against E. coli at pH 4, 5, 6, and 7. Treatment of the extract with detergents, lipase and lysozyme eliminated its antimicrobial activity. Conclusions: Our study gives an indication of the presence of promising antimicrobial compounds and provides basic information about the nature of the Elaeagnus angustifolia extract. Future studies should elucidate the components responsible for antimicrobial activity of these extracts against target cultures.

Chemistry, Pharmacology and Medicinal Property of Russian olive (Elaeagnus angustifolia L.)

“…The study of Elaeagnus angustifolia showed that the MCI (minimum inhibitory concentration) value of the fruit extract which indicates the antimicrobial activity against the targeted culture ranged from 7.5 mg/mL to 0.1 mg/mL. Above all, strong antimicrobial activity with MIC of 1.62 mg/ mL against E. coli was shown in this study [34]. The report signifies the presence of promising antimicrobial agents in the E. angustifolia fruit extracts.…”

“…During the recent years, due to the increased rate of illnesses caused by antibiotic-resistant microorganisms, the importance of using better alternatives such as medicinal plants with their natural antimicrobial activity has grown significantly [34].…”

“…The report signifies the presence of promising antimicrobial agents in the E. angustifolia fruit extracts. Further studies are needed for finding the compounds responsible for the antimicrobial activity of the extract in targeted cultures [34].…”

Phytocomplex Characterization and Biological Evaluation of Powdered Fruits and Leaves from Elaeagnus angustifolia

“…Moreover, Elaeagnus fruit and leaf extracts were both tested as antimicrobial agents on antibiotic-resistant microorganisms, showing good activity towards Escherichia coli and Salmonella typhimurium [17,18]. Finally, Elaeagnus polysaccharides were investigated for the best extraction conditions and their physicochemical and functional properties, and have been shown to exert anti-diabetic, antitumor, and immunological effects [19].…”