Characterization of an Antiserum Against Human Myeloma Cells2

Staneck, L. D.; Krueger, Robert G.; Kyle, Robert A.

doi:10.1093/jnci/53.4.927

Cited by 3 publications

(7 citation statements)

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“…The RPMI 8226 cells have the following types of biological properties: HLA BI 3 antigen (Associated Biomedic Systems); produce A light chains (Staneck et al, 1974;Moore and Kitamura, 1968;Matsuoka et al, 1967); possess cytoplasmic markers for y-, x-, and I-chains (Staneck et a/., 1974); are negative for the presence of Epstein-Barr virus (EBV) particles, EBV capsid antigen, EBV nuclear antigen (EBNA) or EBV DNA sequences in the cells' genome (Minowada et al, 1974). The 8226 cell line was derived by culture of peripheral leukocytes from a patient with IgG-1 myeloma (Matsuoka et al, 1967).…”

Section: D L Culturementioning

confidence: 99%

“…Antiseruni to RPMI 4098 cells was generated according to the same protocol. The antisera were exhaustively absorbed with normal human bone-marrow cells as previously described until they did not give a significant immunofluorescence reaction on smears of normal human bonemarrow cells (Staneck et d., 1974). We demonstrated previously that this antiserum had no reactivity for human immunoglobulins, light or heavy chains (Staneck e t a / .…”

Section: Generation Of Antiseramentioning

confidence: 99%

“…The indirect immunofluorescence assay with viable and fixed cells was carried out as previously described (Staneck et al, 1974) using goat anti-rabbit IgC conjugated with fluorescein isothiocyanate.…”

Section: Serologic Assaysmentioning

confidence: 99%

“…Cells wcre cultured in RPMI 1640 medium supplemented with 20 heat-inactivated fetal calf serum and 0.4 % glutamine at 37" C in a 5 % C0,-95% air environment. The RPMl 4098 cell line (normal human lymphocytes) was obtained from Associated Biomedic Systems, Inc., Buffalo, New York and grown in Dulbecco's modified Eagle's medium supplemented with 10 % heat-inactivated fetal calf serum and 0.4% glutamine at 37" C in a 5 % COB-95 % air environment.The RPMI 8226 cells have the following types of biological properties: HLA BI 3 antigen (Associated Biomedic Systems); produce A light chains (Staneck et al, 1974;Moore and Kitamura, 1968; Matsuoka et al, 1967) …”

mentioning

confidence: 99%

“…The generation of a rabbit antiserum to a tissue culture line of human myeloma cells (RPMI 8226) and its reactivity with a number of different types of cells, including bone-marrow cells from normal individuals and patients with multiple myeloma, were described in previous reports (Staneck et al, 1974;Krueger et a/., 1976). The data suggested that the antibodies in the serum reacted with an antigen(s) only present o n human myeloma cells (i.e., malignant human plasma cells).…”

mentioning

confidence: 99%

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Partial characterization of an antigen from a human myeloma cell and its reactivity with an anti‐myeloma cell serum

Krueger

Hilton

1979

Intl Journal of Cancer

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This report describes studies carried out in an attempt t o define the antibody specificity of a rabbit antiserum generated t o the RPMl8226 line of human myeloma cells and then isolate and characterize the antigen. The antiserum reacted t o a significantly higher t i t e r in a quantitative semi-micro comp- The generation of a rabbit antiserum to a tissue culture line of human myeloma cells (RPMI 8226) and its reactivity with a number of different types of cells, including bone-marrow cells from normal individuals and patients with multiple myeloma, were described in previous reports (Staneck et al., 1974; Krueger et a/., 1976). The data suggested that the antibodies in the serum reacted with an antigen(s) only present o n human myeloma cells (i.e., malignant human plasma cells). O n the basis of this reactivity it was suggested that these cells possess a tumorspecific antigen. Recently, MacKenzie and Paglierone (1977) published data that also suggested that human myeloma plasma cells possessed a tumorspecific antigen(s). These authors showed that bonemarrow plasma cells from myeloma patients stimulated autologous and allogeneic peripheral blood lymphocytes (PBL) from myeloma patients in mixed leukocyte culture. Further, an isolated antigen preparation from myeloma plasma cells elicited proliferative and cytotoxic responses with lymphocytes from myeloma patients but not from nonmyeloma patients.The report describes studies carried out to define the antibody specificity of a rabbit antiserum generated to human myeloma cells as well as the results of our initial studies on the solubilization and isolation of the antigens of a line of human myeloma cells. MATERIAL A N D MtTHODS C d l cultureThe RPMI 8226 cell line was obtained from Dr. George Moore, Denver General Hospital, Denver, Colorado. Cells wcre cultured in RPMI 1640 medium supplemented with 20 heat-inactivated fetal calf serum and 0.4 % glutamine at 37" C in a 5 % C0,-95% air environment. The RPMl 4098 cell line (normal human lymphocytes) was obtained from Associated Biomedic Systems, Inc., Buffalo, New York and grown in Dulbecco's modified Eagle's medium supplemented with 10 % heat-inactivated fetal calf serum and 0.4% glutamine at 37" C in a 5 % COB-95 % air environment.The RPMI 8226 cells have the following types of biological properties: HLA BI 3 antigen (Associated Biomedic Systems); produce A light chains (Staneck et al., 1974;Moore and Kitamura, 1968; Matsuoka et al., 1967)

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Section: D L Culturementioning

confidence: 99%

Section: Generation Of Antiseramentioning

confidence: 99%

Section: Serologic Assaysmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Partial characterization of an antigen from a human myeloma cell and its reactivity with an anti‐myeloma cell serum

Krueger

Hilton

1979

Intl Journal of Cancer

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Monoclonal IgM, IgA and IgG in the serum of a single individual: Immunofluorescence identification of cells producing the immunoglobulins

Krueger¹,

Fair

Kyle

1979

Eur J Immunol

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Monoclonal IgM(ϰ) and IgA(ϰ) in the serum of patient CM were previously shown to share identical, individually specific (Ind) or idiotypic antigenic determinants. A component in the IgG fraction of CM's serum was shown by one of the assay systems to also share Ind determinants with the IgA and IgM. Recent experiments have indicated that the CM IgG monoclonal protein also shares identical Ind determinants with the other two CM immunoglobulins (Ig). These results suggested that the variable regions of the heavy and light chains of the three different classes of Ig were very similar. A direct immunofluorescence assay was used in this study on bone marrow smears of patient CM obtained at two different dates to identify the cells producing these Ig. The reaction of antisera specific for Ind determinants demonstrated that most plasma cells, whether containing IgM, IgA, or IgG, stained with the anti‐Ind antisera; this occurred irrespective of whether the anti‐Ind antisera were generated to the patient's IgM or IgA. The reaction with isotypic antisera revealed cell populations containing either IgM, IgA, or IgG and a significant cell population containing both IgM and IgA. The common occurrence of IgM and IgA‐containing cells which synthesize monoclonal Ig with shared Ind determinants provides evidence consistent with the IgM‐IgA pathway of differentiation of antibody‐producing cells. Further, the tendency toward decreasing numbers of IgM‐staining cells with concomitant increasing numbers of IgA and IgG‐containing cells suggests that the clones of cells producing these Ig were derived from a single precursor cell.

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Circulating Lymphocytes and the Spread of Myeloma

Warner

Krueger

1978

The Lancet

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Characterization of an Antiserum Against Human Myeloma Cells2

Cited by 3 publications

References 0 publications

Partial characterization of an antigen from a human myeloma cell and its reactivity with an anti‐myeloma cell serum

Partial characterization of an antigen from a human myeloma cell and its reactivity with an anti‐myeloma cell serum

Monoclonal IgM, IgA and IgG in the serum of a single individual: Immunofluorescence identification of cells producing the immunoglobulins

Circulating Lymphocytes and the Spread of Myeloma

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