The transfer of 226Ra from irrigation water to basil crops was studied in field conditions. A dedicated basil plot was established and divided into test and control subplots irrigated with water having high (2.1 Bq L−1) and low (0.05 Bq L−1) activity concentrations of 226Ra, respectively. The experiment was performed over a period of 18 months during the autumn, winter and spring seasons, altogether eight cycles of growth and harvest. The activity concentration of 226Ra in basil grown in the test subplots was found to increase from a value of 0.6 Bq kg−1 up to 5.1 Bq kg−1 with successive cycles, compared to a mean value of 0.2 Bq kg−1 for basil grown in the control subplots. The increase in activity concentration of 226Ra in basil grown in the test subplots is mainly attributed to its build-up in the soil in which the level of 226Ra was found to increase by ~ 40%. The effective uptake of 226Ra from the irrigation water (via soil) by the basil plants was found to be approximately 0.4%. The maximal radiation dose following consumption of basil crops grown in the test subplots is negligible (~3 μSv/y).