Characterization of an Effective Frankia Isolate from Nodules of Discaria trinervis

Chaia, Eugenia; Valverde, Claudio; Vobis, Gernot; Wall, Luis Gabriel

doi:10.1007/978-94-011-5159-7_214

Cited by 21 publications

(31 citation statements)

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“…The authors reported, that 5 months old plants developed a restricted number of nodules, in the case of experimental plants up to 5 nodules, and 9 for field plants. It is to emphasize, when Frankia strain BCU110501 was originally used to inoculate plants growing in tubes as used in the present assays, it showed a high capacity to infect D. trinervis, which developed 13 nodules per plant on an average, after 3 months (Chaia 1998). Beside, in own previous experiments, the number of nodules reached 17 per plant, if inoculated with Frankia alone (Solans et al 2004).…”

Section: Discussionmentioning

confidence: 89%

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Discaria trinervis – Frankia symbiosis promotion by saprophytic actinomycetes

Solans

2007

J. Basic Microbiol.

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The influence of saprophytic actinomycetes strains on the Discaria trinervis - Frankia actinorhizal symbiosis was investigated. Three strains out of 122 isolated from the rhizosphere and rhizoplane of D. trinervis with multiple enzymatic activities, were selected for plant growth experiments: Streptomyces (BCRU-MM40), Actinoplanes (BCRU-ME3) and Micromonospora (BCRU-MM18). Inoculated seedlings of Discaria trinervis were grown in glass tubes with vermiculite-sand for 12 weeks. They were inoculated either with a single saprophytic strain or a combination of one or two of them together with the symbiotic N(2) fixing strain Frankia BCU110501. The saprophytic strains were applied in two experimental series, i.e. mycelium + supernatant simultaneously or mycelium and supernatant (growth medium free of cells) separately. Micromonospora strain MM18 showed a direct promotion effect on shoot growth, when plants were inoculated with mycelium and supernatant together. Streptomyces strain MM40 and Actinoplanes strain ME3 promoted the actinorhizal symbiosis with Frankia and consequently the development of plant shoots, when supernatant was involved as inoculum. It is supposed, that the strains MM18, MM40 and ME3 produce bioactive metabolites, which are released into the culture medium. The saprophytic strains studied could be considered as "promoting or helper rhizoactinomycetes" of the actinorhizal plant D. trinervis.

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Section: Discussionmentioning

confidence: 89%

“…Strain BCU110501 was isolated from root nodules of D. trinervis, demonstrating infectivity and effectivity to its own host plant (Chaia 1998). The symbiosis has been well characterized by using different types of growth systems.…”

Section: Discussionmentioning

confidence: 99%

Discaria trinervis – Frankia symbiosis promotion by saprophytic actinomycetes

Solans

2007

J. Basic Microbiol.

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“…and effective (Fix?) in its proper host plant species (Chaia 1998), was used as inoculum as symbiotic N 2-fixing bacteria. All strains are stored at the culture collection of the BCRU Herbarium, Department of Botany, Centro Regional Universitario Bariloche, Universidad Nacional del Comahue, S. C. de Bariloche, Argentina.…”

Section: Actinomycete Strainsmentioning

confidence: 99%

Production of phytohormones by root-associated saprophytic actinomycetes isolated from the actinorhizal plant Ochetophila trinervis

Solans

Vobis

Cassán

et al. 2011

World J Microbiol Biotechnol

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The aim of the present study was to evaluate phytohormone production by symbiotic and saprophytic actinomycetes isolated from the actinorhizal plant Ochetophila trinervis which had previously proved to stimulate nodulation by Frankia. Three saprophytic strains out of 122, isolated from the rhizosphere of this plant with multiple enzymatic activities were selected for plant growth experiments in pots: Streptomyces sp. (BCRU-MM40), Actinoplanes sp. (BCRU-ME3) and Micromonospora sp. (BCRU-MM18). For experiments, the symbiotic N 2 -fixing strain Frankia (BCU110501), isolated from nodules of the same actinorhizal plant was used. Phytohormone production was evaluated in supernatant of non-inoculated and inoculated culture media in exponential growth phase. Indole 3-acetic acid (IAA) and gibberellic acid (GA 3 ) were analyzed by gas chromatography-mass spectrometry (GC-MS), while zeatine (Z) production was determined by gas chromatography-flame ionization detector and high performance liquid chromatography (HPLC fluorescent and UV). The levels of the three phytohormones produced by the saprophytic rhizoactinomycetes were higher than that produced by the symbiotic Frankia strain. Zeatine biosynthesis was higher (lg ml -1 ) than IAA and GA 3 (ng ml -1 ), and Micromonospora strain produced the highest levels of these phytohormones. Although O. trinervis has been shown to be intercellularly infected by Frankia without mediation of root hair deformation, when plants were co-inoculated with actinomycetes' culture, some root hair deformation was observed. This is the first report on identification of IAA, GA 3 and Z in saprophytic actinomycetes and their potential role in plant-microbe interaction.

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“…Frankia strain BCU110501 is an isolate from D. trinervis nodules that is infective (Nod + ) and effective (Fix + ) in D. trinervis plants (Chaia, 1998). The inoculum was prepared from bacteria grown in BAP defined medium supplemented with 1% (w/v) of glucose as C source, without shaking, at 28°C for 4 weeks (Chaia, 1998).…”

Section: Frankia Growth and Plant Inoculationmentioning

confidence: 99%

Effects of calcium in the nitrogen-fixing symbiosis between actinorhizal Discaria trinervis (Rhamnaceae) and Frankia

2009

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We have analysed the growth and symbiotic performance of actinorhizal Discaria trinervis at various Ca supply regimes. We aimed at discriminating between specific, if any, effects on nodulation and general growth stimulation by Ca. The hypothesis that a high Ca supply would interfere with nodulation by Frankia was also tested. Results showed that plant growth increased with Ca supply. Nodulation was stimulated by moderate levels of Ca, but inhibited by Ca higher than 0.77 mM. Growth of nodules was less affected by Ca than shoot and root growth. Ca concentration of symbiotic plants increased with Ca supply, but nitrogen concentration was independent of Ca at concentrations which did not impair plant growth. All together, these results show that Ca has a positive effect on the establishment and functioning of the symbiosis between Discaria trinervis and Frankia. However, the positive influence of Ca was more likely due to a promotion of plant growth rather than a direct effect on nodule growth and nitrogen fixation itself. At high levels of Ca supply nodulation was impaired. Given the intercellular infection pathway in Discaria trinervis, we suggest that the increment of Ca availability would strengthen its root cell walls, thus decreasing Frankia penetration of the root.

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Characterization of an Effective Frankia Isolate from Nodules of Discaria trinervis

Cited by 21 publications

References 0 publications

Discaria trinervis – Frankia symbiosis promotion by saprophytic actinomycetes

Discaria trinervis – Frankia symbiosis promotion by saprophytic actinomycetes

Production of phytohormones by root-associated saprophytic actinomycetes isolated from the actinorhizal plant Ochetophila trinervis

Effects of calcium in the nitrogen-fixing symbiosis between actinorhizal Discaria trinervis (Rhamnaceae) and Frankia

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