Characterization of an immobilized hexose oxidase reactor for mono- and oligosaccharide determination by liquid chromatography

Pyranose Oxidase Modified Carbon Paste Electrodes for Monosaccharide Determination

“…A number of these have been used in conjunction mainly with analytical flow systems for the detection of some mono-, di-, and oligosaccharides. Examples of such enzymes are oligosaccharide dehydrogenase [6][7][8], aldose dehydrogenase [9], cellobiose dehydrogenase (formerly denoted cellobiose oxidase) [7,10], and hexose oxidase [11]. In this article, yet another enzyme with a broad enzyme specificity is used, viz.…”

Amperometric Biosensors for Detection of Sugars Based on the Electrical Wiring of Different Pyranose Oxidases and Pyranose Dehydrogenases with Osmium Redox Polymer on Graphite Electrodes

“…There are a number of other sugar oxidizing enzymes with bound cofactors. Some of them have been previously used for biosensor constructions, e.g., hexose oxidase [7], oligosaccharide dehydrogenase [8 -10], aldose dehydrogenase [11], cellobiose dehydrogenase [12,13], carbohydrate oxidase [14] and pyranose oxidase [15,16]. In the present work, electrical wiring of various forms of two different sugar oxidizing enzymes, i.e., pyranose oxidase (P2O), both wild type and a mutant, as well as one commercial P2O, and additionally pyranose dehydrogenase (PDH) from two different origins, with an osmium redox polymer, (poly(1-vinylimidazole) 12 -[Os(4,4'-dimethyl-2,2'-dipyridyl) 2 Cl 2 ] 2þ/þ ), is reported.…”

Oligosaccharide Dehydrogenase-Modified Graphite Electrodes for the Amperometric Determination of Sugars in a Flow Injection System

“…Systems for postcolumn detection of the substrates of some of these enzymes have been described in the literature. 3,6,14,15,19,21 However, these systems are either too selective or not long-term stable.Oligosaccharide dehydrogenase (ODH) is active for a considerable number of mono-, di-, and oligosaccharides. The first ODHbased amperometric biosensors consisted of p-benzoquinone mixed in carbon paste electrodes with ODH adsorbed on the surface and covered with a polymer membrane.…”

“…Systems for postcolumn detection of the substrates of some of these enzymes have been described in the literature. 3,6,14,15,19,21 However, these systems are either too selective or not long-term stable.…”

“…The great interest in the selective detection of sugars in complex matrixes, such as biotechnical products, fermentation broths, starch, and wood hydrolysates, has put focused research on the use of group-selective sugar oxidizing enzymes in enzyme-based detection systems. To this category of enzymes with broad substrate selectivity belong galactose oxidase, , glucose dehydrogenase, cellobiose dehydrogenase (formerly denoted cellobiose oxidase), − aldose dehydrogenase, − hexose oxidase, pyranose oxidase, and oligosaccharide dehydrogenase. − The potential use of these enzymes for the selective detection of sugars in complex matrixes is realized if enzymatic detection methods are coupled to chromatographic methods. Systems for postcolumn detection of the substrates of some of these enzymes have been described in the literature. ,,,,, However, these systems are either too selective or not long-term stable.…”