Characterization of an Krox-24/Egr-1-responsive element in the human tumor necrosis factor promoter

Introduction of DNA enzyme for Egr-1 into tubulointerstitial fibroblasts by electroporation reduced interstitial α-smooth muscle actin expression and fibrosis in unilateral ureteral obstruction (UUO) rats

“…Egr-1 has been implicated in the induced expression of growth factors such as TGF-␤, 16 PDGF-B, 20 and basic fibroblast growth factor. 17 Cytokines, such as TNF-␣, 31 and adhesion molecules, such as ICAM-1 21 can be affected. Recently, activation of Egr-1 was shown to function as a master switch activated by ischemia to trigger expression of pivotal regulators of inflammation, coagulation and vascular hyperpermeability.…”

Basic fibroblast growth factor transcriptional autoregulation requires EGR-1

“…Interestingly, Sp1 binds to a sequence that overlaps both of these Egr-1 sites. Overlapping Egr-1 and Sp1 binding sites have also been found in promoters of the Egr-1 (Cao et al, 1993), the tumor necrosis factor a (Kramer et al, 1994), the human IL-2 (Skerka et al, 1995), the human platelet-derived growth factors (PDGF) Achain (Khachigian et al, 1995), PDGF-B (Khachigian et al, 1996), the human NF-KB1 (Cogswell et al, 1997), the human tissue factor (Cui et al, 1996), the mouse adenosine deaminase (Ackermann et al, 1991), the mouse thrombospondin 1 (Shingu and Bornstein, 1994) and the rat phenylethanolamine N-methyltransferase genes (Ebert and Wong, 1995). It appears that binding of Sp1 and Egr-1 are mutually exclusive.…”

The Wilms' tumor gene product represses the transcription of thrombospondin 1 in response to overexpression of c-Jun

“…The expression vectors used were: pRSV-c-Jun (Bine truy et al, 1991), pCMV ± KROX24 for EGR1 (Kramer et al, 1994), pCMV(WT17KTS) and pCMV(WT1+KTS) for the WT1 isoforms. Mock transfections were performed using an empty expression vector or a CMV ± EGFP plasmid.…”