Characterization of an L-Arabinose Isomerase from Bacillus velezensis and Its Application for L-Ribulose and L-Ribose Biosynthesis

Guo, Zongren; Long, Liangkun; Ding, Shaojun

doi:10.1007/s12010-020-03380-0

Cited by 4 publications

(1 citation statement)

References 54 publications

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“…This would include the conversion of maltose to maltitol. Production of L-ribulose would, however, suggest a microbial and enzymatic conversion [49]. Similarly the formation of inositol happens through enzymatic phosphorylation of glucose to glucose phosphate (see the residues in Figure 9) followed by isomerisation of glucose phosphate to inositol-phosphate and finally dephosphorylation to inositol [50].…”

Section: Sugarsmentioning

confidence: 99%

Behaviour of Extractives in Norway Spruce (Picea abies) Bark during Pile Storage

et al. 2022

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The current practices regarding the procurement chain of forest industry sidestreams, such as conifer bark, do not always lead to optimal conditions for preserving individual chemical compounds. This study investigates the standard way of storing bark in large piles in an open area. We mainly focus on the degradation of the most essential hydrophilic and hydrophobic extractives and carbohydrates. First, two large 450 m3 piles of bark from Norway spruce (Picea abies) were formed, one of which was covered with snow. The degradation of the bark extractives was monitored for 24 weeks. Samples were taken from the middle, side and top of the pile. Each sample was extracted at 120 °C with both n-hexane and water, and the extracts produced were then analysed chromatographically using gas chromatography with flame ionisation or mass selective detection and high-performance liquid chromatography. The carbohydrates were next analysed using acidic hydrolysis and acidic methanolysis, followed by chromatographic separation of the monosaccharides formed and their derivatives. The results showed that the most intensive degradation occurred during the first 4 weeks of storage. The levels of hydrophilic extractives were also found to decrease drastically (69% in normal pile and 73% in snow-covered pile) during storage, whereas the decrease in hydrophobic extractives was relatively stable (15% in normal pile and 8% in snow-covered pile). The top of the piles exhibited the most significant decrease in the total level of extractives (73% in normal and snow-covered pile), whereas the bark in the middle of the pile retained the highest amount of extractives (decreased by 51% in normal pile and 47% in snow-covered pile) after 24-week storage.

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Section: Sugarsmentioning

confidence: 99%

Behaviour of Extractives in Norway Spruce (Picea abies) Bark during Pile Storage

et al. 2022

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The Industrial Application Potential of Sugar Beet Pulp Derived Monosaccharides d‐Galacturonic Acid and l‐Arabinose.

Jansen,

Hendriks,

Bentlage

et al. 2024

ChemBioChem

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This review provides a perspective on the industrial application potential of sugar beet pulp (SBP) derived monosaccharides. The broad application of these monosaccharides could contribute to bio‐based alternatives and sustainable practices, essential for the transition towards a more circular economy. This review focuses on the utilization and application of two SBP monosaccharides, d‐galacturonic acid (d‐GalA) and l‐arabinose (l‐Ara), derived from pectin and hemicellulose. These polysaccharides are major components of sugar beet pulp, an important side stream of sucrose production. d‐GalA and l‐Ara are therefore abundant in biomass and offer unique molecular structures amenable to selective chemical or enzymatic modifications. We review their application in various industrial applications such as the development and production of bioactive compounds, home and personal care products, and other industries.

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Preparation of Bio‐Orthogonal Self‐Cross‐Linked Arabinose Isomerase for Enzymatic Synthesis of ʟ‐Ribulose

Luo,

Wu,

Luo

et al. 2024

ChemistrySelect

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Abstractʟ‐Ribulose is a bioactive molecule of significance that is present in various valuable chemical scaffolds. However, the synthesis of ʟ‐ribulose is limited by the high cost of enzyme biocatalyst. In this work, we leveraged the self‐coupling reactivity of SpyTag/SpyCatcher to aggregate the key arabinose isomerase (araA) for the enzymatic isomerization of ʟ‐arabinose to ʟ‐ribulose. The cross‐linking of SpyTag/SpyCatcher fused araA was simplified by performing in cell lysates without further purification. The resulting araA cross‐linked aggregates (araA‐CLEs) inherited their original activity well with 0.32 U·mg−1. Deep analysis of araA‐CLEs by Gaussian fitting indicated a little change in secondary structure, mainly focusing on α‐helix. The araA‐CLEs maintained 69.10% catalytic activity after six repeated uses in preparing ʟ‐ribulose. This one‐step cross‐linking and purification method contributed greatly to obtaining araA‐CLEs, thus, benefiting the full use of araA protein and saving costs for ʟ‐ribulose production.

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Characterization of an L-Arabinose Isomerase from Bacillus velezensis and Its Application for L-Ribulose and L-Ribose Biosynthesis

Cited by 4 publications

References 54 publications

Behaviour of Extractives in Norway Spruce (Picea abies) Bark during Pile Storage

Behaviour of Extractives in Norway Spruce (Picea abies) Bark during Pile Storage

The Industrial Application Potential of Sugar Beet Pulp Derived Monosaccharides d‐Galacturonic Acid and l‐Arabinose.

Preparation of Bio‐Orthogonal Self‐Cross‐Linked Arabinose Isomerase for Enzymatic Synthesis of ʟ‐Ribulose

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