Characterization of an Na+/K+/Cl− co-transport in primary cultures of rat astrocytes

Tas, Piet W.L.; Massa, Paul T.; Kress, Hans G.; Koschel, Klaus

doi:10.1016/0005-2736(87)90047-2

Cited by 78 publications

(44 citation statements)

References 37 publications

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“…Nevertheless, neither the amplitude nor the kinetics of the K+-induced a 1 increases were affected by furosemide. Similar results were obtained with bumetanide which seems to be a more potent inhibitor on Na+-K+{Cl-co-transport than furosemide in cultured astrocytes as well as in non-neuronal tissues (Tas, Massa, Kress & Koschel, 1987). In the bath, most of the Cl--sensitive microelectrodes did not respond to bumetanide at concentrations below 0 5 mm.…”

Section: Effects Of Cl--free Solutions On Em and Ac1supporting

confidence: 81%

“…Furosemide, an inhibitor of Na+-K+Cl-cotransport in a variety oftissues (Hoffman, 1986), established a passive Cl-distribution in these oligodendrocytes (Hoppe & Kettenmann, 1988). In addition, cultured mammalian astrocytes as well as LRM 55 glioma cells apparently have a low resting Cl-conductance as a prerequisite for active Cl-accumulation via Na+-K+ Cl-cotransport and Cl--HC03-exchange (Kimelberg, Biddlecome & Bourke, 1979;Tas et al 1987;Wolpaw & Martin, 1984). In these cells, such inwardly directed Cl-pumps establish an EC, about 20-40 mV more positive than Em (e.g.…”

Section: Chloride Distributionmentioning

confidence: 99%

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Intracellular chloride activity in glial cells of the leech central nervous system.

Ballanyi

Schlue

1990

The Journal of Physiology

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Section: Effects Of Cl--free Solutions On Em and Ac1supporting

confidence: 81%

Section: Chloride Distributionmentioning

confidence: 99%

Intracellular chloride activity in glial cells of the leech central nervous system.

Ballanyi

Schlue

1990

The Journal of Physiology

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“…As, Astrocytic process; Ax, unmyelinated axons. Scale bars, 0.5 m. Kimelberg and Frangakis, 1985;Walz and Hinks, 1985;Ballanyi et al, 1987;Tas et al, 1987;Walz, 1987;Dietzel et al, 1989), and active uptake by Na ϩ ,K ϩ ATPase (Walz and Hertz, 1982;Reichenbach et al, 1986Reichenbach et al, , 1992Ballanyi et al, 1987;Dietzel et al, 1989).…”

Section: Activity-dependent Ion Fluxes and Osmotic Gradients In The Rmentioning

confidence: 99%

Aquaporin-4 Water Channel Protein in the Rat Retina and Optic Nerve: Polarized Expression in Müller Cells and Fibrous Astrocytes

Nagelhus¹,

Veruki

Torp³

et al. 1998

J. Neurosci.

411

358

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The water permeability of cell membranes differs by orders of magnitude, and most of this variability reflects the differential expression of aquaporin water channels. We have recently found that the CNS contains a member of the aquaporin family, aquaporin-4 (AQP4). As a prerequisite for understanding the cellular handling of water during neuronal activity, we have investigated the cellular and subcellular expression of AQP4 in the retina and optic nerve where activity-dependent ion fluxes have been studied in detail. In situ hybridization with digoxigenin-labeled riboprobes and immunogold labeling by a sensitive postembedding procedure demonstrated that AQP4 and AQP4 mRNA were restricted to glial cells, including Mü ller cells in the retina and fibrous astrocytes in the optic nerve. A quantitative immunogold analysis of the Mü ller cells showed that these cells exhibited three distinct membrane compartments with regard to AQP4 expression. End feet membranes (facing the vitreous body or blood vessels) were 10-15 times more intensely labeled than non-end feet membranes, whereas microvilli were devoid of AQP4. These data suggest that Mü ller cells play a prominent role in the water handling in the retina and that they direct osmotically driven water flux to the vitreous body and vessels rather than to the subretinal space. Fibrous astrocytes in the optic nerve similarly displayed a differential compartmentation of AQP4. The highest expression of AQP4 occurred in end feet membranes, whereas the membrane domain facing the nodal axolemma was associated with a lower level of immunoreactivity than the rest of the membrane. This arrangement may allow transcellular water redistribution to occur without inducing inappropriate volume changes in the perinodal extracellular space.

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“…Concerning the NKCC dissociation constants, the values were estimated simulating experimental data. Tas et al (1987) measured, in rat brain astrocytes, the Rb + influx, promoted by these cotransporters in dependence on the extracellular concentrations of Na + , K + and Cl -. The measurements were carried out for cells incubated in Na + -free solution (A), in [K + ] = 1 mM (B) and in Cl --free solution (C).…”

Section: Mathematical Descriptionmentioning

confidence: 99%

Biophysical Aspects of the Nonsynaptic Epileptiform Activity

Almeida¹,

Rodrigues²,

Duarte³

et al. 2011

Underlying Mechanisms of Epilepsy

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Characterization of an Na+/K+/Cl− co-transport in primary cultures of rat astrocytes

Cited by 78 publications

References 37 publications

Intracellular chloride activity in glial cells of the leech central nervous system.

Intracellular chloride activity in glial cells of the leech central nervous system.

Aquaporin-4 Water Channel Protein in the Rat Retina and Optic Nerve: Polarized Expression in Müller Cells and Fibrous Astrocytes

Biophysical Aspects of the Nonsynaptic Epileptiform Activity

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