Characterization of an Oct1 orthologue in the channel catfish, Ictalurus punctatus: A negative regulator of immunoglobulin gene transcription?

Lennard, Mara L.; Hikima, Jun-ichi; Ross, David A.; Kruiswijk, Corine P.; Wilson, Melanie; Miller, Norman W.; Warr, Gregory W.

doi:10.1186/1471-2199-8-8

Cited by 12 publications

(5 citation statements)

References 51 publications

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“…These results reinforce that gene stability could be influenced by different factors, such as age, tissue and conditions [35,49]. Furthermore, the HPRT1 gene was classified as the least stable gene by the three software, although this gene has already been considered a good reference gene for different swine tissues [54] and mice [55], and is widely used in rats as a reference gene in qPCR studies.…”

Section: Plos Onementioning

confidence: 57%

“…However, Nakayama et al [ 51 ] and Nascimento et al [ 35 ] found similar results from our study, where the RPLP1 was suitable for normalization of gene expression in nasal tissue in humans and muscle tissue in broilers, respectively. Furthermore, some studies that evaluated the stability of the HMBS gene found this gene to be one of the most stable when considering several tissues [ 34 , 35 , 49 , 52 – 54 ]. Zhang et al [ 52 ], for example, evaluating the stability of eight reference genes in 10 types of Boer goat tissues found that the HMBS gene was the third most stable and therefore recommended this gene for calibrating gene expression analyses of goat tissues from this breed by real-time qPCR.…”

Section: Discussionmentioning

confidence: 99%

“…Zhang et al [53] showed that HMBS was the most suitable gene for chickens gut, while Paludo et al [34] used HMBS as an endogenous gene to study bones from broilers with 45 days of age affected with femoral head necrosis. This gene has been used as endogenous in many species, in different tissues and ages [34,49,54]. Here, the HMBS gene had a good classification in the Bestkeeper tool, but not in NormFinder and GeNorm (Table 2).…”

Section: Plos Onementioning

confidence: 97%

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Reference genes for proximal femoral epiphysiolysis expression studies in broilers cartilage

et al. 2020

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The use of reference genes is required for relative quantification in gene expression analysis and the stability of these genes can be variable depending on the experimental design. Therefore, it is indispensable to test the reliability of endogenous genes previously to their use. This study evaluated nine candidate reference genes to select the most stable genes to be used as reference in gene expression studies with the femoral cartilage of normal and epiphysiolysis-affected broilers. The femur articular cartilage of 29 male broilers with 35 days of age was collected, frozen and further submitted to RNA extraction and quantitative PCR (qPCR) analysis. The candidate reference genes evaluated were GAPDH, HMBS, HPRT1, MRPS27, MRPS30, RPL30, RPL4, RPL5, and RPLP1. For the gene stability evaluation, three software were used: GeNorm, BestKeeper and NormFinder, and a global ranking was generated using the function RankAggreg. In this study, the RPLP1 and RPL5 were the most reliable endogenous genes being recommended for expression studies with femur cartilage in broilers with epiphysiolysis and possible other femur anomalies.

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Section: Plos Onementioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 97%

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Reference genes for proximal femoral epiphysiolysis expression studies in broilers cartilage

et al. 2020

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“…RAD50 is a crucial protein enrolled in DNA repair [ 42 ]. RTEL is a DNA helicase which functions in the stability, protection and elongation of telomeres [ 43 ]. TERC is a critical component of telomerase complex, which provides RNA template for the telomere elongation [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of promoter methylation and expression of telomere related genes in breast cancer

et al. 2017

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Telomeres at the ends of eukaryotic chromosomes play a critical role in tumorgenesis. Using microfluidic PCR and next-generation bisulfite sequencing technology, we investigated the promoter methylation of 29 telomere related genes in paired tumor and normal tissues from 184 breast cancer patients. The expression of significantly differentially methylated genes was quantified using qPCR method.We observed that the average methylation level of the 29 telomere related genes was significant higher in tumor than that in normal tissues (P = 4.30E-21). A total of 4 genes (RAD50, RTEL, TERC and TRF1) showed significant hyper-methylation in breast tumor tissues. RAD51D showed significant methylation difference among the four breast cancer subtypes. The methylation of TERC showed significant association with ER status of breast cancer. The expression profiles of the 4 hyper-methylated genes showed significantly reduced expression in tumor tissues. The integration analysis of methylation and expression of these 4 genes showed a good performance in breast cancer prediction (AUC = 0.947).Our results revealed the methylation pattern of telomere related genes in breast cancer and suggested a novel 4-gene panel might be a valuable biomarker for breast cancer diagnosis.

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“…Octamer transcription factor 1 (Oct1; gene symbol pou2f1 ), a homeologous transcription factor of Oct4 [ 4 ], is comprised in the Pit-Oct-Unc (Pit1, Oct1/2, Unc86) family, which possesses similar in vitro DNA binding specificity [ 5 ]. Through binding to the canonical DNA motif (ATGCAAAT), OCT1 affects the transcription of a number of genes, like the immunoglobulin genes in B lymphocytes [ 6 , 7 ], some interleukins [ 8 – 10 ], and Pit-1 [ 11 ]. As a cis -factor, OCT1 is also required in embryogenesis and maintaining stem cell function in vivo [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Elevated OCT1 participates in colon tumorigenesis and independently predicts poor prognoses of colorectal cancer patients

et al. 2015

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Octamer transcription factor 1 (OCT1) was found to influence the genesis and progression of numerous cancers except for colorectal cancer (CRC). This study tried to explore the role of OCT1 in CRC and clarify the association between its expression and patients’ clinical outcome. Transcriptional and post-transcriptional expression of OCT1 was detected in CRC cancerous tissues and paired normal mucosae by real-time PCR as well as immunohistochemistry. Moreover, the effect of OCT1 knockdown on CRC cell proliferation was investigated both in vitro and in vivo using Cell Counting Kit-8 assay, colony-forming assay, and mouse tumorigenicity assay. Expression of OCT1 was found to be elevated in CRC. Suppression of OCT1 significantly inhibited CRC cell proliferation both in vitro and in vivo. Furthermore, upregulated level of OCT1 was significantly associated with N stage, M stage, and American Joint Committee on Cancer (AJCC) stage (P = 0.027, 0.014, and 0.002, respectively) as well as differential degree (P = 0.022). By using multivariate Cox hazard model, OCT1 was also shown to be a factor independently predicting overall survival (OS; P = 0.013, hazard ratio = 2.747, 95 % confidence interval 1.125 to 3.715) and disease-free survival (DFS; P = 0.004, hazard ratio = 2.756, 95 % confidence interval 1.191 to 4.589) for CRC patients. Our data indicate that OCT1 carries weight in colorectal carcinogenesis and functions as a novel prognostic indicator and a promising target of anti-cancer therapy for CRC.

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Characterization of an Oct1 orthologue in the channel catfish, Ictalurus punctatus: A negative regulator of immunoglobulin gene transcription?

Cited by 12 publications

References 51 publications

Reference genes for proximal femoral epiphysiolysis expression studies in broilers cartilage

Reference genes for proximal femoral epiphysiolysis expression studies in broilers cartilage

Integrated analysis of promoter methylation and expression of telomere related genes in breast cancer

Elevated OCT1 participates in colon tumorigenesis and independently predicts poor prognoses of colorectal cancer patients

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