Characterization of an oleate 12-desaturase from Physaria fendleri and identification of 5′UTR introns in divergent FAD2 family genes

Lozinsky, Sharla; Yang, Hui; Forseille, Li; Cook, Gillian; Ramirez-Erosa, Irving; Smith, Mark A.

doi:10.1016/j.plaphy.2013.12.016

Cited by 11 publications

(10 citation statements)

References 34 publications

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“…The 59 UTR intron is a conserved feature of FAD2 genes (Kim et al, 2006;Lozinsky et al, 2014). Kim et al (2006) have shown that the 59 UTR introns of both sesame (Sesamum indicum) and Arabidopsis FAD2 mediate the enhancement of gene expression.…”

Section: Positionmentioning

confidence: 99%

Genome Wide Analysis of Fatty Acid Desaturation and Its Response to Temperature

et al. 2017

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Section: Positionmentioning

confidence: 99%

Genome Wide Analysis of Fatty Acid Desaturation and Its Response to Temperature

et al. 2017

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“…A double presence of the pentamer CGATT as part of IME signals, the very near location of the intron (11bp) to the translational starting site and the duplications within the sequence probably could contribute overall to enhance the gene expression level (Chung et al, 2006;Lozinsky et al, 2014). The SNP frequency detected in the 5′UTR intron was lower than those found in intron region of genes belonging to the same primary biosynthetic pathway such as acyl carrier protein (ACP) genes (Cultrera et al, 2019), even if this author found on average a higher SNP frequency than other species.…”

Section: Discussionmentioning

confidence: 99%

“…Free from the constraints of encoding proteins, UTRs can form considerable Watson-Crick and non-canonical base pairing that can potentially impact every step of translation (Leppek et al, 2018). Despite the evolutive conservation of the 5′ UTR intron, the high structural variability found among and within the species makes difficult to speculate about a specific regulation mechanism (Lozinsky et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

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Association Study of the 5′UTR Intron of the FAD2-2 Gene With Oleic and Linoleic Acid Content in Olea europaea L.

et al. 2020

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Cultivated olive (Olea europaea L. subsp. europaea var. europaea) is the most ancient and spread tree crop in the Mediterranean basin. An important quality trait for the extra virgin olive oil is the fatty acid composition. In particular, a high content of oleic acid and low of linoleic, linolenic, and palmitic acid is considered very relevant in the health properties of the olive oil. The oleate desaturase enzyme encoding-gene (FAD2-2) is the main responsible for the linoleic acid content in the olive fruit mesocarp and, therefore, in the olive oil revealing to be the most important candidate gene for the linoleic acid biosynthesis. In this study, an in silico and structural analysis of the 5′UTR intron of the FAD2-2 gene was conducted with the aim to explore the natural sequence variability and its role in the gene expression regulation. In order to identify functional allele variants, the 5′ UTR intron was isolated and partially sequenced in 97 olive cultivars. The sequence analysis allowed to find a 117-bp insertion including two long duplications never found before in FAD2-2 genes in olive and the existence of many intron-mediated enhancement (IME) elements. The sequence polymorphism analysis led to detect 39 SNPs. The candidate gene association study conducted for oleic and linoleic acids content revealed seven SNPs and one indel significantly associated able to explain a phenotypic variation ranging from 7% to 16% among the years. Our study highlighted new structural variants within the FAD2-2 gene in olive, putatively involved in the regulation mechanisms of gene expression associated with the variation of the content of oleic and linoleic acid.

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“…Heterologous expression is usually performed to characterize new functional isoforms in many species, such as soybean (Li et al 2007), Physaria fendleri (Lozinsky et al 2014) and Elaeis guineenses (Sun et al 2016). This method was used to evaluate the effect of an aspartate for asparagine exchange at position 150 of one FAD2 desaturase in Arachis hypogaea (Bruner et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a new GmFAD3A allele in Brazilian CS303TNKCA soybean cultivar

et al. 2018

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We molecularly characterized a new mutation in the GmFAD3A gene associated with low linolenic content in the Brazilian soybean cultivar CS303TNKCA and developed a molecular marker to select this mutation. Soybean is one of the most important crops cultivated worldwide. Soybean oil has 13% palmitic acid, 4% stearic acid, 20% oleic acid, 55% linoleic acid and 8% linolenic acid. Breeding programs are developing varieties with high oleic and low polyunsaturated fatty acids (linoleic and linolenic) to improve the oil oxidative stability and make the varieties more attractive for the soy industry. The main goal of this study was to characterize the low linoleic acid trait in CS303TNKCA cultivar. We sequenced CS303TNKCA GmFAD3A, GmFAD3B and GmFAD3C genes and identified an adenine point deletion in the GmFAD3A exon 5 (delA). This alteration creates a premature stop codon, leading to a truncated protein with just 207 residues that result in a non-functional enzyme. Analysis of enzymatic activity by heterologous expression in yeast support delA as the cause of low linolenic acid content in CS303TNKCA. Thus, we developed a TaqMan genotyping assay to associate delA with low linolenic acid content in segregating populations. Lines homozygous for delA had a linolenic acid content of 3.3 to 4.4%, and the variation at this locus accounted for 50.83 to 73.70% of the phenotypic variation. This molecular marker is a new tool to introgress the low linolenic acid trait into elite soybean cultivars and can be used to combine with high oleic trait markers to produce soybean with enhanced economic value. The advantage of using CS303TNKCA compared to other lines available in the literature is that this cultivar has good agronomic characteristics and is adapted to Brazilian conditions.

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Characterization of an oleate 12-desaturase from Physaria fendleri and identification of 5′UTR introns in divergent FAD2 family genes

Cited by 11 publications

References 34 publications

Genome Wide Analysis of Fatty Acid Desaturation and Its Response to Temperature

Genome Wide Analysis of Fatty Acid Desaturation and Its Response to Temperature

Association Study of the 5′UTR Intron of the FAD2-2 Gene With Oleic and Linoleic Acid Content in Olea europaea L.

Characterization of a new GmFAD3A allele in Brazilian CS303TNKCA soybean cultivar

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