Characterization of an Oxidative Stress Inducible Nonspecific Lipid Transfer Protein Coding cDNA and its Promoter from Drought Tolerant Plant Prosopis juliflora

George, Suja; Parida, Ajay

doi:10.1007/s11105-009-0127-y

Cited by 18 publications

(7 citation statements)

References 54 publications

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“…They included enzymes involved in protein degradation, water channel proteins, protease inhibitors, LEA proteins, cytochrome P450, plasma membrane aquaporins, heat shock proteins, lipid transfer proteins, and plant defense-related genes. LEA proteins may function in protecting macromolecules, such as enzymes, protein complexes, and membranes (Wang et al 2003;Khurana et al 2008;George and Parida 2010). Water channel proteins are thought to function in the transport of water through plasma membranes and tonoplasts to adjust the osmotic pressure (Sakurai et al 2005;Zhang et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling of Dehydration Stress in the Chinese Cabbage (Brassica rapa L. ssp. pekinensis) by Tag Sequencing

Zhang

et al. 2011

Plant Mol Biol Rep

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Chinese cabbage (Brassica rapa L. ssp. pekinensis) is a vegetable crop with high water requirement and is adversely affected by drought during cultivation. A genome-wide transcription analysis in response to drought is essential to provide effective genetic engineering strategies to improve stress tolerance in crop plants. To gain a deeper understanding of the mechanisms of drought tolerance in B. rapa, we conducted the first genome-wide analysis of gene expression during a drought model using tag sequencing with a Solexa Illumina array. Leaf samples at four time points, 0 day, 1 day, 2 days, and 3 days after dehydration treatment, were taken from a B. rapa DH line, T12-19, and the corresponding RNA was used to construct the expression libraries. The total number of tags per library ranged from 5.6 to 7.6 million and, among these, 13,036, 12,472, 12,774, and 12,227 distinct clean tags for the four respective libraries were unambiguously mapped to a publicly available unigene database. The analysis of differentially expressed genes revealed that 1,092 genes were significantly altered in response to water deficit. In this set of genes, we found 37 transcription factors, 28 genes involved in signal transduction, and 61 water-and osmosensing-responsive genes. Overall, the tag sequencing analyses demonstrated a high degree of transcriptional complexity in response to dehydration stress and represents a considerable improvement over microarray data in the large-scale analysis of transcriptional changes.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling of Dehydration Stress in the Chinese Cabbage (Brassica rapa L. ssp. pekinensis) by Tag Sequencing

Zhang

et al. 2011

Plant Mol Biol Rep

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“…The expression pattern of nsLTPs from different plants has shown a large divergence covering various tissues and against environmental stimulates (George and Parida 2010;Kader 1996;Pitzschke et al 2013;Pyee et al 1994). Consistent with their complex expression profiles, the pleiotropic functions of nsLTPs have been previously suggested to be involved in cuticle synthesis (Lee et al 2009;Pyee et al 1994), catabolism in lipid storage (Tsuboi et al 1992), somatic embryogenesis (Kader 1996), stigmapollen interaction (Huang et al 2013;Mollet et al 2000;Tian et al 2013), defense signaling (Blein et al 2002;Maldonado et al 2002), nodule organogenesis (Lei et al 2014) development, and antimicrobial activities (Caaveiro et al 1997;Kristensen et al 2000).…”

Section: Introductionmentioning

confidence: 86%

Identification of wheat non-specific lipid transfer proteins involved in chilling tolerance

Hou

et al. 2014

Plant Cell Rep

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Three TaLTPs were found to enhance chilling tolerance of transgenic Arabidopsis, which were characterized by analyzes of promoter-GUS activity, subcellular localization, chromosomal location and transcriptional profile. Non-specific lipid transfer proteins (nsLTP) are abundantly expressed in plants, however, their functions are still unclear. In this study, we primarily characterized the functions of 3 type I TaLTP genes that were localized on chromosomes 3A, 3B, and 5D, respectively. The transcripts of TaLTPIb.1 and TaLTPIb.5 were induced under chilling, wound, and drought conditions, while TaLTPId.1 was only up-regulated by dark treatment. All the 3 TaLTP genes could be stimulated by the in vitro treatment of salicylic acid, while TaLTPId.1 was also positively regulated by methyljasmonic acid. Furthermore, the promoter-reporter assay of TaLTPIb.1 in the transgenic brachypodium showed a typical epidermis-specific expression pattern of this gene cluster. When fused with EGFP, all the 3 proteins were shown to localize on the plasma membrane in transgenic tobacco, although a signal in chloroplasts was also observed for TaLTPId.1. Heterogeneous overexpression of each of the TaLTP genes in Arabidopsis resulted in longer root length compared with wild type plants under chilling condition. These results suggest that type I TaLTPs may have a conserved functionality in chilling tolerance by lipid permeation in the plasma membrane of epidermal cells. On the other hand, the type I TaLTPs may exert functional divergence mainly through regulatory subfunctionalization.

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“…Lipid transfer proteins are involved in plant defense against environmental changes and phytopathogens (George and Parida 2010). Thioredoxin M participates in oxidative stress tolerance in plants (Broin and Rey 2003).…”

Section: Discussionmentioning

confidence: 99%

Reducing the allelopathic effect of Parthenium hysterophorus L. on wheat (Triticum aestivum L.) by Pseudomonas putida

Mishra

Nautiyal

2011

Plant Growth Regul

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The present study evaluates the role of Pseudomonas putida NBRIC19 in alleviating biotic stress of Parthenium hysterophorus (Parthenium) in Triticum aestivum. Due to presence of Parthenium there was 43.76, 53.08 and 78.65% inhibition in root length, shoot length and dry weight of wheat respectively. This inhibition was recovered when P. putida NBRIC19 treatment resulted in 52.29, 28.73 and 76.31% increase in root length, shoot length and dry weight respectively as compared to control. P. putida NBRIC19 was able to form more biofilm under toxic environment of allelochemicals and enhanced expression of stress responsive genes in wheat. Inoculated wheat plants showed lower activity of catalase and ascorbate peroxidase under biotic stress of Parthenium indicating that inoculated plants felt less stress as compared to uninoculated plants. Microbial community structure in bacterized and nonbacterized wheat rhizosphere in presence and absence of Parthenium, was investigated using Biolog. There was significant increase in microbial diversity in P. putida NBRIC19 bacterized wheat rhizosphere. Functional microbial diversity revealed that P. putida NBRIC19 had shifted the microflora in such a manner that utilization of phytotoxic allelochemicals increased to lessen its toxic effect and finally it resulted in better growth of wheat in presence of Parthenium. Principal component analysis showed that microbial community function in nonbacterized wheat rhizosphere in presence (WPC) and absence (WC) of Parthenium is totally different from each other but due to P. putida NBRIC19 treatment there was close clustering of WPT and WT indicating a total shift in microbial community structure.

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Characterization of an Oxidative Stress Inducible Nonspecific Lipid Transfer Protein Coding cDNA and its Promoter from Drought Tolerant Plant Prosopis juliflora

Cited by 18 publications

References 54 publications

Transcriptome Profiling of Dehydration Stress in the Chinese Cabbage (Brassica rapa L. ssp. pekinensis) by Tag Sequencing

Transcriptome Profiling of Dehydration Stress in the Chinese Cabbage (Brassica rapa L. ssp. pekinensis) by Tag Sequencing

Identification of wheat non-specific lipid transfer proteins involved in chilling tolerance

Reducing the allelopathic effect of Parthenium hysterophorus L. on wheat (Triticum aestivum L.) by Pseudomonas putida

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