Characterization of an ST5-SCCmec II-t311 methicillin-resistant Staphylococcus aureus strain with a widespread cfr-positive plasmid

Wu, Dang; Wang, Haiping; Zhu, Feiteng; Jiang, Shengnan; Sun, Lu; Zhao, Feng; Yu, Yunsong; Chen, Yan

doi:10.1016/j.jiac.2020.02.018

Cited by 8 publications

(8 citation statements)

References 27 publications

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“…Another plasmid, pSX01 (KP890694), was detected in a Staphylococcus xylosus strain recovered from a pig in Henan, China in 2015. The other two plasmids, pSR01 30 and pXWZ_1 31 (KP890694), were detected in MRSA and S. capitis strains, respectively, as reported by our group. As shown in Supplementary Fig.…”

Section: Discussionsupporting

confidence: 83%

Development of a novel core genome MLST scheme for tracing multidrug resistant Staphylococcus capitis

Wang

Sun

et al. 2022

Nat Commun

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Staphylococcus capitis, which causes bloodstream infections in neonatal intensive care units, is a common cause of healthcare-associated infections. Thus, a standardized high-resolution typing method to document the transmission and dissemination of multidrug-resistant S. capitis isolates is required. We aimed to establish a core genome multilocus sequence typing (cgMLST) scheme to surveil S. capitis. The cgMLST scheme was defined based on primary and validation genome sets and tested with outbreaks of linezolid-resistant isolates and a validation set. Phylogenetic analysis was performed to investigate the population structure and compare it with the result of cgMLST analysis. The S. capitis population consists of 1 dominant, NRCS-A, and 4 less common clones. In this work, a multidrug-resistant clone (L clone) with linezolid resistance is identified. With the features of type III SCCmec and multiple copies of mutations of G2576T and C2104T in the 23S rRNA, the L clone has been spreading silently across China.

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Section: Discussionsupporting

confidence: 83%

Development of a novel core genome MLST scheme for tracing multidrug resistant Staphylococcus capitis

Wang

Sun

et al. 2022

Nat Commun

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“…The highest BLASTN hit for pSauR165-1 are plasmids pSR02 from the MRSA strain SR153 (accession no. CP048645) that was isolated in Hangzhou, China, in 2013 [ 119 ], and pT8G from the Staphylococcus lugdunensis strain Tlug8G-4 (accession no. KU882684.1) that was isolated from Hong Kong at about the same time period [ 120 ].…”

Section: Resultsmentioning

confidence: 99%

“… Comparative linear maps of the multidrug-resistant pSauR165-1 plasmid with its close relatives pSR02 plasmid from MRSA strain SR153 (accession no. CP048645; [ 119 ]) and pT8G from Staphylococcus lugdunensis strain Tlug8G-4 (accession no. KU882684.1; [ 120 ]).…”

Section: Figurementioning

confidence: 99%

The Plasmidomic Landscape of Clinical Methicillin-Resistant Staphylococcus aureus Isolates from Malaysia

et al. 2023

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Methicillin-resistant Staphylococcus aureus (MRSA) is a priority nosocomial pathogen with plasmids playing a crucial role in its genetic adaptability, particularly in the acquisition and spread of antimicrobial resistance. In this study, the genome sequences of 79 MSRA clinical isolates from Terengganu, Malaysia, (obtained between 2016 and 2020) along with an additional 15 Malaysian MRSA genomes from GenBank were analyzed for their plasmid content. The majority (90%, 85/94) of the Malaysian MRSA isolates harbored 1–4 plasmids each. In total, 189 plasmid sequences were identified ranging in size from 2.3 kb to ca. 58 kb, spanning all seven distinctive plasmid replication initiator (replicase) types. Resistance genes (either to antimicrobials, heavy metals, and/or biocides) were found in 74% (140/189) of these plasmids. Small plasmids (<5 kb) were predominant (63.5%, 120/189) with a RepL replicase plasmid harboring the ermC gene that confers resistance to macrolides, lincosamides, and streptogramin B (MLSB) identified in 63 MRSA isolates. A low carriage of conjugative plasmids was observed (n = 2), but the majority (64.5%, 122/189) of the non-conjugative plasmids have mobilizable potential. The results obtained enabled us to gain a rare view of the plasmidomic landscape of Malaysian MRSA isolates and reinforces their importance in the evolution of this pathogen.

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“…Another interesting observation was the strains with the presence of the cfr gene, shown in this review (). The Cfr resistance mechanism causes post-transcriptional methylation of the 23S rRNA affecting drugs belonging to several antimicrobial lasses, and it has already been shown that it can be carried by plasmids [16, 87]. As highlighted here, the biofilm plays an auxiliary role in the transfer of mobile genetic elements, which may contribute to the spread of, for example, plasmids containing the cfr gene.…”

Section: Future Perspectivementioning

confidence: 98%

“…The cfr gene was detected in isolates from humans, cattle, meat products and the environment and was identified in a variety of plasmids including the pSCFS3 plasmid of S. aureus isolated from swine species [87,88]. In another study by Osman et al, the authors emphasize the limitation of focusing the studies only on animal-origin MRSA and the lack of data for other resistance genes and their investigation involving the formation of biofilms [16,89].…”

Section: Studies On S Aureus Biofilm In the Meat Chainmentioning

confidence: 99%

Staphylococcus aureus biofilm: the role in disseminating antimicrobial resistance over the meat chain

et al. 2022

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Staphylococcus aureus is responsible for severe skin and respiratory infections and food poisoning, resulting in hospitalizations and high morbidity worldwide. Staphylococci have extensive virulence mechanisms and antimicrobial resistance that pose a global challenge to contain the spread of infectious outbreaks. Antimicrobials are used as growth promoters, and for prevention and treatment of infections in animals that provide us with food. The improvement of animal health is undeniable, but the selection of multidrug-resistant strains that can spread resistance genes among microorganisms is undesirable. The administration of sublethal doses of antimicrobials in farm animals causes stress to Staphylococci inducing the formation of a complex extracellular polymeric structure called biofilm. Such a structure may favor the persistence of infection by disseminating antimicrobial-resistant strains that can be consumed in contaminated food of animal origin. In ruminant mastitis and hospitals, the potential of the biofilm structure in the persistence of infections, especially those caused by S. aureus , has already been demonstrated, as well as its role as a source of resistant genes. In the meat production chain, the potential for persistent contamination by biofilm structure is evidently a worrying health risk . This review brings together studies demonstrating that biofilm production facilitates the exchange of mobile genetic elements and random mutations in S. aureus strains within the structure. This contributes to the emergence of more resistant clonal complexes and, with biofilm support, persists in the meat production chain.

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Characterization of an ST5-SCCmec II-t311 methicillin-resistant Staphylococcus aureus strain with a widespread cfr-positive plasmid

Cited by 8 publications

References 27 publications

Development of a novel core genome MLST scheme for tracing multidrug resistant Staphylococcus capitis

Development of a novel core genome MLST scheme for tracing multidrug resistant Staphylococcus capitis

The Plasmidomic Landscape of Clinical Methicillin-Resistant Staphylococcus aureus Isolates from Malaysia

Staphylococcus aureus biofilm: the role in disseminating antimicrobial resistance over the meat chain

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