Characterization of an Unusual DS-1-Like G8P[8] Rotavirus Strain from Japan in 2017: Evolution of Emerging DS-1-Like G8P[8] Strains through Reassortment

Kamiya, Hajime; Tacharoenmuang, Ratana; Ide, Tomihiko; Negoro, Manami; Tanaka, Takaaki; Asada, Kazutoyo; Nakamura, Haruna; Sugiura, Katsumi; Umemoto, Masakazu; Kuroki, Haruo; Ito, Hiroaki; Tanaka, Shigeki; Ito, Mitsue; Fukuda, Seisuke; Hatazawa, Riona; Hara, Yuya; Guntapong, Ratigorn; Murata, Takayuki; Taniguchi, Kiyosu; Suga, Shigeru; Nakano, Takashi; Taniguchi, Koki; Komoto, Satoshi

doi:10.7883/yoken.jjid.2018.484

Cited by 15 publications

(14 citation statements)

References 15 publications

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“…Two G8P [8] strains (Tokyo16-4476 and Tokyo17-20) were reassorted with VP1 of DS-1-like G1P [8] strains (data not shown). These strains were similar to the SO1162 strain detected in Chiba, Japan, in 2017 (37). Three DS-1-like G3P [8] strains (Tokyo16-3861, Tokyo17-09, and Tokyo17-119) were reassorted with VP6 of G2P [4] strains (data not shown).…”

Section: Discussionsupporting

confidence: 74%

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Molecular Characteristics of Novel Mono-Reassortant G9P[8] Rotavirus A Strains Possessing the NSP4 Gene of the E2 Genotype Detected in Tokyo, Japan

et al. 2020

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INTRODUCTION Rotavirus A (RVA) is a major cause of gastroenteritis in infants and young children worldwide. In 2016, RVA caused 128,500 deaths in children under 5 years of age globally (1). Although most of these deaths occurred in developing countries, RVA imposes a huge burden even in developed countries, including Japan (2-4). Live attenuated RVA vaccines were introduced in Japan in 2011 (Rotarix ® , GlaxoSmithKline Biologicals, Rixensart, Belgium) and in 2012 (RotaTeq ® , Merck & Co., Inc., Kenilworth, NJ, USA), and while RVA vaccines have proven to be effective (5,6), the selective pressure of a vaccine may induce an epidemic strain shift (7-10). The RVA genome contains 11 gene segments of double-stranded RNA that encode six structural (VPs) and six nonstructural proteins (NSPs) (11). To classify RVAs, a specific genotype is assigned to each of the 11 genome segments, according to predefined nucleotide sequence identity cutoff values (12-14). The classification system denotes the VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 6 genes of an RVA strain as a descriptor Gx-P[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx (x indicating genotype number),

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Section: Discussionsupporting

confidence: 74%

“…Typical human G3P [8] with a Wa-like genotype constellation resulted in a small epidemic. The equine-like G3P [8] and G8P [8] strains have been detected in several regions in Japan recently (25,27,36,37), suggesting they are beginning to circulate across the country.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characteristics of Novel Mono-Reassortant G9P[8] Rotavirus A Strains Possessing the NSP4 Gene of the E2 Genotype Detected in Tokyo, Japan

et al. 2020

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“…Similarly, in the postvaccine era in Japan, the predominant RV genotype changed to DS-1-like G1P[8] in 2012 [21–23] and, since 2014, G8P[8] has emerged and become sporadically dominant [2, 28, 29]. Since 2013, eG3 strains have been sporadically detected in Japan [10, 13, 32], and this study reports the first outbreak of eG3 in Japan during 2016 to 2017.…”

Section: Discussionmentioning

confidence: 91%

Molecular and clinical characterization of the equine-like G3 rotavirus that caused the first outbreak in Japan, 2016

Akane

Tsugawa

Fujii

et al. 2021

Journal of General Virology

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Since 2013, equine-like G3 rotavirus (eG3) strains have been detected throughout the world, including in Japan, and the strains were found to be dominant in some countries. In 2016, the first eG3 outbreak in Japan occurred in Tomakomai, Hokkaido prefecture, and the strains became dominant in other Hokkaido areas the following year. There were no significant differences in the clinical characteristics of eG3 and non-eG3 rotavirus infections. The eG3 strains detected in Hokkaido across 2 years from 2016 to 2017 had DS-1-like constellations (i.e. G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2), and the genes were highly conserved (97.5–100 %). One strain, designated as To16-12 was selected as the representative strain for these strains, and all 11 genes of this strain (To16-12) exhibited the closest identity to one foreign eG3 strain (STM050) seen in Indonesia in 2015 and two eG3 strains (IS1090 and MI1125) in another Japanese prefecture in 2016, suggesting that this strain might be introduced into Japan from Indonesia. Sequence analyses of VP7 genes from animal and human G3 strains found worldwide did not identify any with close identity (>92 %) to eG3 strains, including equine RV Erv105. Analysis of another ten genes indicated that the eG3 strain had low similarity to G2P[4] strains, which are considered traditional DS-1-like strains, but high similarity to DS-1-like G1P[8] strains, which first appeared in Asia in 2012. These data suggest that eG3 strains were recently generated in Asia as mono-reassortant strain between DS-1-like G1P[8] strains and unspecified animal G3 strains. Our results indicate that rotavirus surveillance in the postvaccine era requires whole-genome analyses.

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“…Of note is that three (VP7, NSP1, and NSP2) and four (VP7, VP1, VP3, and NSP4) genes of strains DBM2017-016 and DBM2018-291, respectively, were assumed to have originated from locally circulating Wa-like G9P [8] and/or DS-1-like G2P [4] strains. Thus, the genomic diversity among Thai DS-1-like G9P [8] intergenogroup reassortant strains detected in the present study appeared to have been generated through additional reassortment with locally circulating RVAs [6,10,13,16,19,22,31,44]. It should also be noted that the VP7 gene of strain DBM2017-203 was closely related to that of contemporary French Wa-like G9P [8] strain Dijon-R10484, however, epidemiological links to Thailand were missing.…”

Section: Discussionmentioning

confidence: 56%

“…On phylogenetic analysis, strain DBM2017-016 formed a cluster with these Spanish equine-like G3P [8] strains (Fig 2D). In contrast, the VP1 gene of strain DBM2017-203 exhibited the maximum nucleotide sequence identity (99.6%) with Japanese bovine-like G8P [8] strain SO1162 [44], Thai DS-1-like G1P [8] strains PCB-180, SKT-109, and SSKT-41 [21], and Vietnamese DS-1-like G1P [8] strain 16020_72 [45] (Table 1 and Fig 1). Phylogenetically, strain DBM2017-203 was found to be very closely related with…”

Section: Plos Onementioning

confidence: 99%

Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand

et al. 2020

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The emergence and rapid spread of unusual DS-1-like intergenogroup reassortant rotaviruses having G1/3/8 genotypes have been recently reported from major parts of the world (Africa, Asia, Australia, Europe, and the Americas). During rotavirus surveillance in Thailand, three novel intergenogroup reassortant strains possessing the G9P[8] genotype (DBM2017-016, DBM2017-203, and DBM2018-291) were identified in three stool specimens from diarrheic children. In the present study, we determined and analyzed the full genomes of these three strains. On full-genomic analysis, all three strains were found to share a unique genotype constellation comprising both genogroup 1 and 2 genes: G9-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis demonstrated that each of the 11 genes of the three strains was closely related to that of emerging DS-1-like intergenogroup reassortant, human, and/or locally circulating human strains. Thus, the three strains were suggested to be multiple reassortants that had acquired the G9-VP7 genes from co-circulating Wa-like G9P[8] rotaviruses in the genetic background of DS-1-like intergenogroup reassortant (likely equine-like G3P[8]) strains. To our knowledge, this is the first description of emerging DS-1-like intergenogroup reassortant strains having the G9P[8] genotype. Our observations will add to the growing insights into the dynamic evolution of emerging DS-1like intergenogroup reassortant rotaviruses through reassortment.

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Characterization of an Unusual DS-1-Like G8P[8] Rotavirus Strain from Japan in 2017: Evolution of Emerging DS-1-Like G8P[8] Strains through Reassortment

Cited by 15 publications

References 15 publications

Molecular Characteristics of Novel Mono-Reassortant G9P[8] Rotavirus A Strains Possessing the NSP4 Gene of the E2 Genotype Detected in Tokyo, Japan

Molecular Characteristics of Novel Mono-Reassortant G9P[8] Rotavirus A Strains Possessing the NSP4 Gene of the E2 Genotype Detected in Tokyo, Japan

Molecular and clinical characterization of the equine-like G3 rotavirus that caused the first outbreak in Japan, 2016

Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand

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