Characterization of angiotensin-converting enzyme expression during epidermis morphogenesis in humans: a potential marker for epidermal stem cells

Hw, Liu; Jf, Li; Huang, Wu; Li, KY; Sun, Tao; Xb, Fu

doi:10.1111/j.1365-2133.2008.08970.x

Cited by 11 publications

(3 citation statements)

References 31 publications

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“…In the early stages of epidermal development, mitotic activity occurs in all the epidermal layers; however, when differentiation begins, mitotic activity becomes restricted to the cells of the basal layer [ 17 ]. Angiotensin converting enzyme (ACE), keratin 19, β1-integrin, and p63 were used as stem cell markers in examining the various developmental stages of fetal skin by double IF [ 19 ]. The expression of ACE, β1-integrin, keratin 19, and p63 was seen in all epidermal layers of the developing fetal skin at 11–20 weeks of gestation.…”

Section: Discussionmentioning

confidence: 99%

CD99 Is Strongly Expressed in Basal Cells of the Normal Adult Epidermis and Some Subpopulations of Appendages: Comparison with Developing Fetal Skin

Choi

Roh

Park

2016

J Pathol Transl Med

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BackgroundCD99 is a cell surface transmembrane glycoprotein expressed in various tissues. CD99 is differentially expressed between subpopulations of each tissue and is highly expressed in certain hematopoietic and precursor cells. However, there has been no comprehensive study of CD99 expression in normal skin. We evaluated CD99 expression in normal human skin and developing fetal skin. MethodsSeventy-five adult skin samples containing normal skin and eight fetal skin samples of different gestational ages were collected. CD99 immunohistochemical staining was performed to evaluate expression pattern in adult and fetal skin samples. CD99 and CD34 expression were compared by double immunofluorescence. ResultsIn normal adult skin, CD99 was strongly expressed in the membrane of epidermal basal keratinocytes, hair follicle bulges and outer root sheaths, and inner secretory cells of eccrine sweat glands. In fetal skin, CD99 was not expressed on the periderm at 16 weeks of gestation but was expressed in basal cells of fetal skin at around 19 weeks of gestation. CD99 expression became comparable to that of the adult skin after 20 weeks of gestation. CD99 and CD34 were co-expressed in hair follicle outer root sheaths, as seen by double immunofluorescence study. ConclusionsThis is the first study examining CD99 expression pattern in normal adult and fetal skin. CD99 tends to be expressed in the basal/precursor cells of epidermis and in hair follicles. These results provide a basis for future investigation on functions of CD99 in the skin and provide a novel potential target for the treatment of dermatologic lesions.

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Section: Discussionmentioning

confidence: 99%

CD99 Is Strongly Expressed in Basal Cells of the Normal Adult Epidermis and Some Subpopulations of Appendages: Comparison with Developing Fetal Skin

Choi

Roh

Park

2016

J Pathol Transl Med

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“…The digested cells were then spun to remove the supernatant, and the pellets were suspended and seeded into a six-well culture plate in DMEM/ F12 medium containing 4 ng/ml bFGF (Thermo Fisher Scientific, Waltham, MA). To identify ESCs, isolated cells were detected using a flow cytometer (Thermo Fisher Scientific, Waltham, MA) with following antibodies: integrin b1 (ab179471, Abcam, Cambridge, UK), keratin 19 (ab191208, Abcam, Cambridge, UK) and keratin 10 (ab76318, Abcam, Cambridge, UK) using a method as previously reported [12,13]. The percentages of integrin b1and KRT19-positive cells were greater than 85%, and the percentage of KRT10positive cells was lesser than 10%.…”

Section: Isolation and Culture Of Escsmentioning

confidence: 99%

Protective mechanism of GPR30 agonist G1 against ultraviolet B-induced injury in epidermal stem cells

Zhang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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The regeneration of the skin is vital to our wound healing and skin repair abilities. Adult epidermal stem cells (ESCs) have been shown to have the potential to renew old and dead skin cells, and ESCs have been implemented in stem cell-based therapies. GPR30 is a G protein-coupled membrane receptor for oestrogen, which has been shown to regulate cell proliferation and programmed cell death. Here, we examined the biological function of GPR30 in isolated adult murine ESCs. We show that GPR30 is fairly expressed in ESCs and is repressed upon ultraviolet B (UV-B) treatment in a dosedependent manner. The activation of GPR30 by its agonist G1 ameliorates UV-B induced cellular oxidative stress and induction of IL-6 and IL-8. Furthermore, G1 protects against UV-B-induced cell death and improves the viability of ESCs. G1 also suppresses UV-B-induced HMGB-1 expression and protects the capacity of ESCs from the harm by UV-B radiation. Mechanistically, we show that co-treatment with G1 rescues UV-B-induced reduced Wnt1, cyclin D1 and b-catenin production, indicating the involvement of conical Wnt/b-catenin. Collectively, our data indicate that the activation of GPR30 has a protective role in ESCs, and GPR30 agonist G1-mediated ESC protection has potential implications in stem cell-based therapies for skin diseases.

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“…Cytokeratin-19 (CK19) is commonly used as a marker of most neuroendocrine and gastrointestinal tumors [20]. CK19 is also known to be a stem cell marker in the liver [21, 22], pancreas [23], and epidermis [24]. In contrast, cytokeratin-14 (CK14), although not as well known as CK19, is used as a marker of the oral epithelium [25-27] and is considered to be a marker of maturation of the epithelial lining [26-28].…”

Section: Introductionmentioning

confidence: 99%

Spatial relationship between expression of cytokeratin-19 and that of connexin-43 in human fetal kidney

et al. 2013

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Connexin-43, a major gap junction protein, and cytokeratin-19, one of the intermediate filament keratins, are known to be markers of well-differentiated epithelium. In this study, we investigated the expression of these markers in the head region, lungs, and abdominal organs of 10 human mid-term fetuses. The expression of connexin-43 was found to be restricted to the dura mater, kidney, and adrenal cortex. In the kidney, we found a clear site-dependent difference in the expression pattern of these markers: connexin-43 expression was observed in the tubules of the renal cortex whereas cytokeratin-19 was strongly expressed in the collecting ducts and renal pelvis. This difference remained unchanged throughout the fetal stages examined. Immunoreactivity was not observed for either of the markers in the intrarenal vessels, including the glomeruli, and mesangial cells. Connexin-43 expression seemed to be restricted to the metanephric vesicle-derived structures that differentiate in the urogenital ridge of the splanchnic mesoderm. The adrenal cortex also originates from the same para-aortic mesoderm. In contrast, in the urogenital organs, cytokeratin-19 seemed to be expressed in ducts derived from the urogenital sinus.

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Characterization of angiotensin-converting enzyme expression during epidermis morphogenesis in humans: a potential marker for epidermal stem cells

Abstract: Our results suggest that ACE may play a role in human epidermis morphogenesis during fetal life and serve as an unrecognized marker for keratinocyte progenitor cells.

Cited by 11 publications

References 31 publications

CD99 Is Strongly Expressed in Basal Cells of the Normal Adult Epidermis and Some Subpopulations of Appendages: Comparison with Developing Fetal Skin

CD99 Is Strongly Expressed in Basal Cells of the Normal Adult Epidermis and Some Subpopulations of Appendages: Comparison with Developing Fetal Skin

Protective mechanism of GPR30 agonist G1 against ultraviolet B-induced injury in epidermal stem cells

Spatial relationship between expression of cytokeratin-19 and that of connexin-43 in human fetal kidney

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