Characterization of anthocyanins in the hybrid progenies derived from Iris dichotoma and I. domestica by HPLC-DAD-ESI/MS analysis

Wang, Xu; Luo, Gangjun; Yu, Fan; Jia, Q. S.; Zheng, Yanan; Bi, Xiaoying; Li, Jiajun

doi:10.1016/j.phytochem.2018.03.003

Cited by 38 publications

(30 citation statements)

References 62 publications

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“…Specifically, the mass spectrum of peak 8 with a retention time at 16 (trans-p-coumaroyl)-5-O-glucoside. The peak 15 with retention time at 32.68 min that had a molecular ion and fragment of 963.2803 and 317 [Pt]+ corresponded to a petunidin aglycone, which was tentatively identified as petunidin-3-(feruloyl)-rutinoside-5-glucoside [21][22][23]. Finally, the peak 17 with a retention time at 36.54 min was identified as malvidin-3-(feruloyl)-rutinoside…”

Section: Identification Of Anthocyanins In the Atpap1-overexpressed Bmentioning

confidence: 99%

Accumulation of Anthocyanins through Overexpression of AtPAP1 in Solanum nigrum Lin. (Black Nightshade)

et al. 2020

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Black nightshade (Solanum nigrum) belongs to the Solanaceae family and is used as a medicinal herb with health benefits. It has been reported that the black nightshade plant contains various phytochemicals that are associated with antitumor activities. Here we employed a genetic approach to study the effects of overexpression of Arabidopsis thaliana production of anthocyanin pigment 1 (AtPAP1) in black nightshade. Ectopic expression of AtPAP1 resulted in enhanced accumulation of anthocyanin pigments in vegetative and reproductive tissues of the transgenic plants. Analysis of anthocyanin revealed that delphinidin 3-O-rutinoside-5-O-glucoside, delphinidin 3,5-O-diglucoside, delphinidin 3-O-rutinoside, petunidin 3-O-rutinoside (cis-p-coumaroyl)-5-O-glucoside, petunidin 3-(feruloyl)-rutinoside-5-glucoside, and malvidin 3-(feruloyl)-rutinoside-5-glucoside are highly induced in the leaves of AtPAP1 overexpression lines. Furthermore, ectopic expression of AtPAP1 evoked expression of early and late biosynthetic genes of the general phenylpropanoid and flavonoid pathways that include phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate CoA ligase (4CL), chalcone isomerase (CHI), and quinate hydroxycinnamoyl transferase (HCT), which suggests these genes might be transcriptional targets of AtPAP1 in black nightshade. Concomitantly, the total content of anthocyanin in the transgenic black nightshade plants was higher compared to the control plants, which supports phenotypic changes in color. Our data demonstrate that a major anthocyanin biosynthetic regulator, AtPAP1, can induce accumulation of anthocyanins in the heterologous system of black nightshade through the conserved flavonoid biosynthesis pathway in plants.

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Section: Identification Of Anthocyanins In the Atpap1-overexpressed Bmentioning

confidence: 99%

Accumulation of Anthocyanins through Overexpression of AtPAP1 in Solanum nigrum Lin. (Black Nightshade)

et al. 2020

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“…On the other hand, cyanidin (2), which is the most common anthocyanidin in nature, pelargonidin (4), and peonidin (6) were found in some cultivars of Iris dichotoma and Iris domestica as major pigments. 144 Although 2 and 6 were also found in the flowers of 7 cultivars of I. ensata as 3-Orutinoside-5-Oglucosides, they rarely occur in I. ensata cultivars. 77 Anthocyanins are not reported in the leaves and rhizomes of Iris species.…”

Section: Anthocyaninsmentioning

confidence: 99%

“…The flower colors of the hybrids derived from these crosses were categorized into 8 groups, violet, brown, orange, red, pink, yellow, and white. 144 Twenty-nine anthocyanins, including glycosides of 1-6, were found in their hybrids. Their anthocyanins were surveyed by high-performance liquid chromatography-diode array detector-electrospray ionization/ mass spectrometry analysis, and it was shown that variation of flower colors of their hybrids is due to qualitative and quantitative differentiation of anthocyanins.…”

Section: Contribution To the Flower Colors Of Flavonoidsmentioning

confidence: 99%

Flavonoids and Xanthones From the Genus Iris: Phytochemistry, Relationships with Flower Colors and Taxonomy, and Activities and Function

Iwashina

Mizuno

2020

Natural Product Communications

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The genus Iris consists of approximately 360 species and is distributed to Eurasia, North Africa, and North America. Belamcanda chinensis was incorporated into the genus Iris by APG III as Iris domestica. Iris species have also been cultivated as ornamentals since ancient times. Many flavonoids are isolated and identified from the flowers, leaves, and rhizomes. So far as we know, 6 anthocyanidins, 13 flavones, 20 flavonols, 20 C-glycosylflavones, 14 flavanones, 11 dihydroflavonols, 6 flavan and proanthocyanidins, and 85 isoflavonoids were reported as aglycones and glycosides. However, chalcones, dihydrochalcones, aurones, biflavonoids, and neoflavonoids are not found in the genus Iris. Fifteen xanthones that are related to flavonoids were also isolated from Iris species. In this review, we describe the flavonoids and xanthones that were isolated and characterized from Iris species. Moreover, their phytochemistry, contribution to the flower colors, relationships with taxonomy, and activities and functions are also discussed.

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“…Compounds 6, 7, 9, 11, and 18 (R ions at m/z 161, 111, 125, and 109, respectively, were identified as protocatechuic hexoside [14,15], citric acid [16], gallic acid [17], and protocatechuic acid [18], respectively. Compound [29], pelargonidin-3-Ohexoside [22], and pelargonidin-3-O-diacetyl hexoside [21], respectively, according to their mass spectra, with a distinctive fragment ion at m/z 271. Peaks 27 and 45 were identified as delphinidin-3-O-feruloyl-hexoside [29,32] and delphinidin-3-O-acetyl rhamnoside, with MS 1 at m/z 641 and 491, respectively, and a common MS 2 fragment at m/z 303, a loss of 338 amu from feruloyl hexoside moiety and of 188 amu from acetyl rhamnoside moiety, respectively [33].…”

Section: Phenolic and Nonphenolic Acidsmentioning

confidence: 99%

Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits

Al‐Yousef

Alqahtani

Hаssаn

et al. 2021

Journal of Chemistry

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Phytochemical and pharmacological investigations of Elettaria cardamomum L. were mostly focused on its essential oil and organic crude extracts with little attention on its aqueous extracts. Therefore, the current study aimed to investigate the phytochemical profile, in vitro antiobesity, and antidiabetic activities of the aqueous extract of Elettaria cardamomum L. family Zingiberaceae. UPLC-ESI-MS/MS analysis is used for the aqueous extract characterization in both ionization modes. The analysis revealed the tentative identification of forty-seven compounds based on their MS/MS fragmentation pattern and comparison with the reported data. The identified compounds include eight flavones, thirteen phenolic and nonphenolic acids, one coumarin, and nineteen anthocyanins. Moreover, the in vitro antiobesity and antidiabetic activities were also studied. The results showed that E. cardamomum L. aqueous extract inhibits pancreatic lipase and α-amylase enzyme in a concentration-dependent manner, as 1 mg/mL extract was able to inhibit pancreatic lipase and α-amylase by 62.25% ± 0.58 (IC50 = 288.75 ± 1.3 µg/mL) and 70.42% ± 1.5 (IC50 = 220.5 ± 1.3 µg/mL), respectively. Conclusively, the current study indicated that the investigated biological activities of Elettaria cardamomum aqueous extract were attributed to the existence of biologically active metabolites such as flavones, phenolic and nonphenolic acids, coumarins, and anthocyanins. Moreover, it proposed that the aqueous cardamom extract can be used as a natural potential source in different pharmaceutical preparations to protect against variable chronic disorders, especially obesity and diabetes. Deeper in vivo investigations, isolation, purification, and structural elucidation of the major active metabolites from cardamom are recommended.

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Characterization of anthocyanins in the hybrid progenies derived from Iris dichotoma and I. domestica by HPLC-DAD-ESI/MS analysis

Cited by 38 publications

References 62 publications

Accumulation of Anthocyanins through Overexpression of AtPAP1 in Solanum nigrum Lin. (Black Nightshade)

Accumulation of Anthocyanins through Overexpression of AtPAP1 in Solanum nigrum Lin. (Black Nightshade)

Flavonoids and Xanthones From the Genus Iris: Phytochemistry, Relationships with Flower Colors and Taxonomy, and Activities and Function

Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits

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